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CN-121467129-B - Preparation method and application of bionic self-perfusion microfluidic chip based on combined driving

CN121467129BCN 121467129 BCN121467129 BCN 121467129BCN-121467129-B

Abstract

The invention discloses a preparation method and application of a bionic self-perfusion microfluidic chip based on joint driving, and belongs to the field of microfluidic chips; the method comprises the steps of forming a substrate layer, embedding a hydrophilic porous material into the reserved groove of the runner layer to form a capillary flow starting area, providing a substrate layer, arranging a hydrophobic porous evaporation film on the surface of the substrate layer at the position of the preset evaporation area, and aligning and bonding the runner layer with the hydrophilic porous material and the substrate layer with the hydrophobic porous evaporation film so that the position of a runner area for evaporation on the runner layer corresponds to the position of the hydrophobic porous evaporation film, thereby obtaining the microfluidic chip. The preparation method of the invention is simple and has low cost.

Inventors

  • DU QIJUN
  • ZHANG FAN
  • WANG SHUQI
  • HU WENQI
  • WU DI
  • LU QINRUI

Assignees

  • 四川迪亚生物科技集团有限公司

Dates

Publication Date
20260505
Application Date
20260105

Claims (8)

  1. 1. The preparation method of the bionic self-perfusion microfluidic chip based on the combined driving is characterized by comprising the following steps of: preparing a runner layer with a reserved groove for accommodating the hydrophilic porous material; Embedding a hydrophilic porous material into the reserved grooves of the runner layer to form a capillary flow starting region; providing a substrate layer, arranging a hydrophobic porous evaporation film on the surface of the substrate layer at the position of a preset evaporation area, and Aligning and bonding the runner layer with the hydrophilic porous material and the substrate layer with the hydrophobic porous evaporation film, so that the runner area for evaporation on the runner layer corresponds to the position of the hydrophobic porous evaporation film, thereby obtaining the microfluidic chip; The hydrophilic porous material is chromatographic filter paper or anisotropic bacterial cellulose/polyvinyl alcohol aerogel, and/or, The hydrophobic porous evaporation membrane is a polytetrafluoroethylene membrane; when the hydrophilic porous material is anisotropic bacterial cellulose/polyvinyl alcohol aerogel, the preparation steps thereof comprise: The preparation of composite slurry, which comprises the steps of mixing bacterial cellulose nanofiber suspension with polyvinyl alcohol solution, adding surfactant, and centrifuging to remove bubbles to obtain uniform slurry; Directional freezing casting, namely placing the slurry into a mould, and controlling a temperature gradient to enable ice crystals growing directionally to be generated in the slurry; Post-treatment, namely freeze-drying the frozen sample to remove ice crystals to form directional pore channels, and performing chemical crosslinking to enhance structural stability; Controlling the vertical upward growth of ice crystals by using liquid nitrogen as a cold source, and fumigating and crosslinking by using glutaraldehyde steam; When the hydrophilic porous material is embedded into the reserved groove, the direction of a micro-channel inside the hydrophilic porous material is parallel to the fluid flowing direction of the runner layer.
  2. 2. The method for preparing a bionic self-perfusion microfluidic chip based on combined driving according to claim 1, wherein the runner layer is made of polydimethylsiloxane.
  3. 3. The method for preparing the bionic self-perfusion microfluidic chip based on the combined driving according to claim 2, wherein the polydimethylsiloxane is prepared by mixing a polydimethylsiloxane prepolymer and a curing agent according to a weight ratio of 10:1 and then thermally curing for 2 hours under the condition of 80 ℃.
  4. 4. The method for preparing the bionic self-perfusion microfluidic chip based on the combined driving according to claim 1, wherein the average pore diameter of the polytetrafluoroethylene membrane is 0.45 μm.
  5. 5. The method for preparing a bionic self-priming microfluidic chip based on combined driving according to claim 1, wherein the alignment bonding comprises plasma treatment of the surface of the flow channel layer and the surface of the basal layer to be bonded to activate the surfaces.
  6. 6. The method for preparing the bionic self-perfusion micro-fluidic chip based on the combined driving according to claim 5, wherein the plasma treatment condition is that the plasma is treated for 45 seconds under the conditions of 50W of power and 100 mTorr of oxygen pressure.
  7. 7. The method for preparing the bionic self-perfusion microfluidic chip based on the combined driving according to claim 1, wherein the preparing method further comprises the following steps: And heating the bonded integral chip at 80 ℃ for 30 minutes to enhance bonding strength.
  8. 8. An application of a bionic self-perfusion microfluidic chip based on joint driving, which is characterized in that the microfluidic chip prepared by the preparation method according to any one of claims 1-7 comprises the following steps: (1) The application in the culture of higher cells and tissues; (2) Application in drug development and toxicology screening.

Description

Preparation method and application of bionic self-perfusion microfluidic chip based on combined driving Technical Field The invention relates to the field of microfluidic chips, in particular to a preparation method and application of a bionic self-perfusion microfluidic chip based on combined driving. Background Microfluidic chip technology, also known as lab-on-a-chip, has great potential in the fields of biology, chemistry, medicine, etc. However, the conventional microfluidic system severely relies on expensive and heavy external pumps to drive the fluid, resulting in problems of low system integration, high power consumption, high cost, difficulty in realizing high-throughput parallel experiments, portability, and the like. In the prior art, some attempts have been made to achieve "self-driven" fluids, such as capillary driving, by utilizing the hydrophilicity of the flow channel surface to create capillary action. However, this method has a limited driving force, is difficult to maintain, and is easily interrupted by evaporation of the liquid. However, this scheme is complicated to construct and the maintenance of the solution concentration is difficult. Accordingly, there is a strong need in the art for a self-priming drive technique that is capable of producing a stable, long-term, force-free self-priming drive. Disclosure of Invention The invention aims to provide a preparation method of a bionic self-perfusion microfluidic chip based on combined driving, which aims to solve the problem that the concentration of a solution is difficult to maintain in the prior art. The invention is realized by the following technical scheme, and the preparation method of the bionic self-perfusion microfluidic chip based on combined driving comprises the following steps: The method comprises the steps of preparing a runner layer with a reserved groove for accommodating a hydrophilic porous material, embedding the hydrophilic porous material into the reserved groove of the runner layer to form a capillary flow starting area, providing a substrate layer, arranging a hydrophobic porous evaporation film on the position of the preset evaporation area on the surface of the substrate layer, and aligning and bonding the runner layer with the hydrophilic porous material and the substrate layer with the hydrophobic porous evaporation film, so that a runner area for evaporation on the runner layer corresponds to the position of the hydrophobic porous evaporation film, thereby obtaining the microfluidic chip. Further, the runner layer is made of polydimethylsiloxane. Further, the polydimethylsiloxane was prepared by mixing a polydimethylsiloxane prepolymer and a curing agent in a weight ratio of 10:1 and thermally curing at 80℃for 2 hours. Further, the hydrophilic porous material is chromatographic filter paper, and/or the hydrophobic porous evaporation membrane is polytetrafluoroethylene membrane. Further, the average pore diameter of the polytetrafluoroethylene film was 0.45. Mu.m. Further, the alignment bonding includes plasma treating the runner layer surface and the substrate layer surface to be bonded to activate the surfaces. Further, the plasma treatment was carried out under the conditions of 50W power and 100 mTorr oxygen pressure for 45 seconds. Further, the preparation method further comprises the step of heating the bonded whole chip at 80 ℃ for 30 minutes to enhance bonding strength. When the hydrophilic porous material is anisotropic bacterial cellulose/polyvinyl alcohol aerogel, the preparation method comprises the steps of preparing composite slurry, namely mixing bacterial cellulose nanofiber suspension with polyvinyl alcohol solution, adding surfactant, centrifuging to remove bubbles to obtain uniform slurry, directionally freezing and casting, namely placing the slurry into a mould, enabling ice crystals to grow directionally in the slurry through controlling a temperature gradient, and performing post-treatment, namely freeze-drying a frozen sample to remove the ice crystals to form directional pore channels, and performing chemical crosslinking to enhance structural stability. Further, liquid nitrogen is used as a cold source to control the vertical upward growth of ice crystals, and glutaraldehyde steam is used for fumigation crosslinking. Further, when the hydrophilic porous material is embedded into the reserved groove, the direction of the micro-channel inside the hydrophilic porous material is parallel to the fluid flow direction of the runner layer. The invention further provides an application of the bionic self-perfusion microfluidic chip based on combined driving, and the bionic self-perfusion microfluidic chip prepared according to the steps has the following application (1) in high-grade cell and tissue culture and (2) in drug research and development and toxicology screening. Compared with the prior art, the invention has the following advantages and beneficial effects: 1. The invention constru