CN-121471319-B - Antibacterial peptide GT-2 and application thereof

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to an antibacterial peptide GT-2 and application thereof. The amino acid sequence of the antibacterial peptide GT-2 is shown as SEQ ID NO. 2. The antibacterial peptide GT-2 provided by the invention is obtained by modifying natural antibacterial peptide GP-1 extracted from gastrodia elata, has the effects of broad-spectrum bacteriostasis, high antibacterial efficiency, low toxicity and high safety, and provides an effective way for providing the antibacterial biological peptide with practicability, high efficiency and safety.

Inventors

• HU JIANGMIAO
• GUO JIATAO
• SUN TIANZHU
• YANG LIU

Assignees

• 中国科学院昆明植物研究所

Dates

Publication Date

20260512

Application Date

20260108

Claims (6)

1. 1. The antibacterial peptide GT-2 is characterized in that the amino acid sequence of the antibacterial peptide GT-2 is shown as SEQ ID NO. 2.
2. 2. Use of an antimicrobial peptide GT-2 according to claim 1 for the preparation of an antimicrobial product, wherein the antimicrobial product is an antibacterial product and an antifungal product; the bacteria are one or more of staphylococcus aureus, methicillin-resistant staphylococcus aureus, escherichia coli and pseudomonas aeruginosa; the fungus is candida albicans.
3. 3. The use of claim 2, wherein the product comprises an agent, and wherein the agent comprises a pharmaceutical product.
4. 4. An antimicrobial product, characterized in that the active ingredient of the antimicrobial product comprises the antimicrobial peptide GT-2 according to claim 1.
5. 5. The antibacterial product according to claim 4, wherein the effective concentration of the antibacterial peptide GT-2 in the antibacterial product is not less than 4 mug/ml.
6. 6. An antimicrobial product according to claim 5, wherein the effective concentration of the antimicrobial peptide GT-2 in the antimicrobial product is 4-32 μg/ml.

Description

Antibacterial peptide GT-2 and application thereof Technical Field The invention belongs to the technical field of biological medicines, and particularly relates to an antibacterial peptide GT-2 and application thereof. Background Natural antimicrobial peptides (Antimicrobial peptides, AMPs) are an important component of the immune system, widely found in animals, plants and microorganisms, and have the ability to kill bacteria, fungi and some viruses. Compared with the traditional antibiotics, the antibacterial peptide plays a role mainly by destroying the cell membrane structure of pathogenic microorganisms or regulating the immune response of hosts, is not easy to induce drug resistance, and has quick effect and multiple targets. Therefore, the antibacterial peptide is considered as one of the most promising antibiotic substitutes. However, natural antimicrobial peptides (AMPs) have broad-spectrum antimicrobial potential, but are still subject to a number of limitations during practical use. Firstly, the antibacterial spectrum of the natural antibacterial peptide is relatively limited, the inhibition capability to drug-resistant pathogens is insufficient, and the requirement of clinical broad-spectrum antibacterial drugs is difficult to meet. Second, the partial natural antibacterial peptide has poor balance between hydrophilicity and hydrophobicity, resulting in insufficient penetration ability when acting with the cell membrane of bacteria, thereby affecting antibacterial efficiency. In addition, natural antibacterial peptide is easily degraded by protease in vivo, has short half-life period, and limits the continuous antibacterial effect. Some natural antimicrobial peptides also exhibit some host cytotoxicity, such as damage to erythrocytes or hepatocytes, thereby reducing their safety. Finally, the extraction and synthesis processes of natural antimicrobial peptides are generally complex and have high cost, which is not beneficial to the large-scale application. Together, these factors lead to major bottlenecks in drug development and clinical conversion of the natural antimicrobial peptides. At present, the optimization of antibacterial peptide drugs mainly focuses on the aspects of improving stability, reducing toxicity, expanding antibacterial spectrum and enhancing targeting, and particularly, the performance of the existing antibacterial peptide is improved through means such as chemical modification, nano-carrier delivery, genetic engineering technology and the like. However, the discovery and optimization of new antimicrobial peptides has been relatively few, and more work has focused on improving and popularizing existing antimicrobial peptides. Therefore, how to provide an antibacterial peptide which is natural in source, has the effects of broad-spectrum bacteriostasis, high antibacterial efficiency, low toxicity and high safety is a problem to be solved in the field. Disclosure of Invention In order to solve the problem that the prior art lacks an antibacterial peptide with less application limit and good antibacterial effect, the invention provides an antibacterial peptide GT-2 and application thereof, and specifically comprises the following technical scheme: the invention provides an antibacterial peptide GT-2, wherein the amino acid sequence of the antibacterial peptide GT-2 is shown as SEQ ID NO. 2. The invention also provides application of the antibacterial peptide GT-2 in preparation of antibacterial products. Preferably, the antimicrobial product includes an anti-bacterial infection product and an anti-fungal infection product. Preferably, the bacteria comprise gram negative bacteria and/or gram positive bacteria. Preferably, the bacteria include one or more of staphylococcus aureus, methicillin-resistant staphylococcus aureus, escherichia coli and pseudomonas aeruginosa. Preferably, the fungus comprises candida albicans. Preferably, the product comprises an agent, which comprises a pharmaceutical product. The invention also provides an antibacterial product, the active ingredients of which comprise the antibacterial peptide GT-2. Preferably, the effective concentration of the antibacterial peptide GT-2 in the antibacterial product is more than or equal to 4 mug/ml. Preferably, the effective concentration of the antibacterial peptide GT-2 in the antibacterial product is 4-32 mug/ml. The invention has the beneficial effects that: The invention provides an antibacterial peptide GT-2, wherein the amino acid sequence of the antibacterial peptide GT-2 is shown as SEQ ID NO. 2. The antibacterial peptide GT-2 is extracted from the traditional medicinal plant gastrodia elata and is modified. The invention extracts peptide fragment information GP-1 with antibacterial activity from gastrodia elata genome, and obtains antibacterial peptide GT-2 with low toxicity and high activity by optimizing the sequence of GP-1. The invention breaks through the development thought of the traditional antib