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CN-121488981-B - Microbial agent for preventing and treating soil-borne diseases of grain crops as well as preparation method and application thereof

CN121488981BCN 121488981 BCN121488981 BCN 121488981BCN-121488981-B

Abstract

The invention discloses a microbial agent for preventing and treating soil-borne diseases of grain crops, and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent for preventing and controlling soil-borne diseases of grain crops comprises, by weight, 40-50 parts of a functional microbial agent, 20-30 parts of a carrier, 5-8 parts of a protective agent, 5-8 parts of a nutritional auxiliary agent and 1-3 parts of a dispersing agent, wherein the functional microbial agent comprises Achromobacter xylosoxidans, streptomyces caucasiaticus and trichoderma hook. The three strains in the microbial agent are matched with synergistic effect, so that the microbial agent has remarkable disease resistance effect, can effectively prevent and treat wheat take-all, wheat sheath blight, rice bakanae disease and false smut, has the best inhibiting effect on the false smut, can effectively solve the problems of high incidence of soil-borne diseases of grain crops, serious continuous cropping obstacle, chemical pesticide pollution and the like, finally realizes the comprehensive effects of disease prevention, yield maintenance, quality improvement, efficiency improvement and environmental protection, and has important significance for guaranteeing the safe production of grains and sustainable development of agriculture.

Inventors

  • ZHOU YIXIN
  • CHEN WANYANG
  • DUAN CHONG
  • FAN DENG
  • LIU ZIRONG
  • ZHANG XIAOYANG
  • LIU YUAN
  • LIU XIAODAN
  • LIU SHENGWU

Assignees

  • 湖北茂盛生物有限公司

Dates

Publication Date
20260505
Application Date
20260112

Claims (7)

  1. 1. The microbial agent for preventing and treating the soil-borne diseases of the grain crops is characterized by comprising the following raw materials, by weight, 40-50 parts of a functional microbial agent, 20-30 parts of a carrier, 5-8 parts of a protective agent, 5-8 parts of a nutritional auxiliary agent and 1-3 parts of a dispersing agent; the preparation method of the functional microbial agent comprises the following steps: (1) Firstly preparing a bacterial suspension from freeze-dried powder of Achromobacter xylosoxidans, dripping 100 mu L of the bacterial suspension onto a nutrient gravy agar medium for culturing for 48 hours, selecting single bacterial colony for inoculating into the nutrient gravy liquid medium, culturing at 30 ℃ and 160rpm until OD 600 = 0.6 to obtain a seed solution, inoculating the seed solution into a small-sized seed tank according to the inoculation amount of 1%, and culturing at 160rpm until the bacterial concentration reaches 1X 10 9 CFU/mL to obtain a fermentation liquor of Achromobacter xylosoxidans; (2) Preparing a bacterial suspension from freeze-dried powder of streptomyces caucasianus, dripping 100 mu L of the bacterial suspension onto a first Gaoshan agar culture medium for 5-7d, selecting single bacterial colony to be inoculated into a first Gaoshan liquid culture medium, carrying out shake culture at 28 ℃ and 200rpm for 60 hours to obtain a seed liquid, inoculating the seed liquid into a small seed tank according to 10% of inoculation amount, and culturing at 200rpm until the bacterial concentration is 1X 10 8 CFU/mL to obtain a streptomyces caucasianus fermentation liquor; (3) Preparing a suspension from freeze-dried powder of trichoderma hook, dripping 100 mu L of the suspension onto a PDA agar plate for culturing until mycelia grow on a culture dish, taking out a fungus cake, inoculating the fungus cake to a potato glucose water culture medium, culturing at 25 ℃ and 180rpm for 6-7d, and regulating the number of spores to 1X 10 8 /mL by using sterile water to obtain trichoderma hook spore suspension; (4) Mixing the Achromobacter xylosoxidans fermentation liquor prepared in the step (1), the streptomyces caucasiaticus fermentation liquor prepared in the step (2) and the trichoderma hook spore suspension prepared in the step (3) according to the volume ratio of 2:1:1 to obtain a functional microbial agent; The preservation number of the Achromobacter xylosoxidans is CGMCC 1.2998, the preservation number of the Streptomyces caucasiaticus is CGMCC 4.1603, and the preservation number of the Trichoderma hook is CGMCC 3.13029.
  2. 2. The microbial agent for preventing and controlling soil-borne diseases of grain crops according to claim 1, wherein the carrier is bran powder and humic acid, and the mass ratio of the bran powder to the humic acid is 1:1.
  3. 3. The microbial agent for preventing and treating soil-borne diseases of grain crops according to claim 1, wherein the protective agent is trehalose and glycerol in a mass ratio of 2:1.
  4. 4. The microbial inoculant for preventing and treating soil-borne diseases of grain crops according to claim 1, wherein the nutritional aid is corn starch.
  5. 5. The microbial inoculant for preventing and treating soil-borne diseases of grain crops according to claim 1, wherein the dispersing agent is sodium lignin sulfonate.
  6. 6. A method for preparing the microbial agent for preventing and treating soil-borne diseases of grain crops according to any one of claims 1 to 5, which is characterized by comprising the following steps: Mixing the protective agent with the prepared functional microbial agent, then sequentially adding the carrier, the nutrition auxiliary agent and the dispersing agent into a mixer, fully stirring uniformly to enable bacterial liquid to be fully adsorbed, drying at 40 ℃ until the water content is lower than 10%, and crushing into powder by a crusher to obtain the final product microbial agent.
  7. 7. The use of a microbial agent for controlling soil-borne diseases of grain crops according to any one of claims 1 to 5, wherein the control of soil-borne diseases of grain crops is specifically control of wheat take-all, wheat sheath blight, bakanae disease of rice and false smut of rice.

Description

Microbial agent for preventing and treating soil-borne diseases of grain crops as well as preparation method and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a microbial agent for preventing and treating soil-borne diseases of grain crops, and a preparation method and application thereof. Background With the popularization of global agriculture intensive planting modes, soil-borne diseases have become key biotic stress restricting the improvement of yield and quality of grain crops. The disease has the characteristics of strong concealment, frequent re-infection, difficult complete eradication by conventional pesticides and the like, and seriously threatens the safe production of main grain crops such as wheat, rice and the like. Soil-borne diseases of grain crops are serious biological disasters which restrict sustainable development of agricultural production, pathogenic bacteria of the crop soil-borne diseases survive in soil for a long time, and can infect root systems and stem bases of the crops, so that various diseases occur in successive years, and serious yield loss and quality reduction are caused. At present, the agricultural production still highly depends on chemical bactericides for soil treatment and disease control, but long-term excessive use not only leads to excessive pesticide residues and pollution to soil and water environments, but also easily causes the enhancement of pathogen resistance, thus forming malignant circulation of 'drug effect reduction-dosage increase', and threatening grain safety and ecological health. In this context, biological control techniques with beneficial microorganisms as the core are an important direction for green control. The microbial agent inhibits pathogenic bacteria by competing multiple mechanisms such as nutrition and space, secretion of antibacterial substances, induction of crop resistance and the like, and has the remarkable advantages of environmental friendliness, difficulty in drug resistance generation and the like. However, the existing microbial agent generally has the problems of single functional strain, narrow disease prevention spectrum, insufficient field stability and the like, and restricts the large-scale popularization and application of the microbial agent. Aiming at the pain spots, the invention creatively screens and compounds functional strains, and aims to realize broad-spectrum inhibition and comprehensive treatment of soil-borne pathogenic bacteria of various grain crops by constructing a multi-bacteria synergistic biocontrol system. Disclosure of Invention The invention aims to provide a microbial agent for preventing and treating soil-borne diseases of grain crops, which is used for effectively preventing and treating wheat take-all, banded sclerotial blight, bakanae disease of rice and false smut of rice. In order to achieve the technical purpose, the invention adopts the following technical scheme: The microbial agent for preventing and treating soil-borne diseases of grain crops comprises the following raw materials, by weight, 40-50 parts of a functional microbial agent, 20-30 parts of a carrier, 5-8 parts of a protective agent, 5-8 parts of a nutritional auxiliary agent and 1-3 parts of a dispersing agent. Further, the functional microbial agent comprises Achromobacter xylosoxidans, streptomyces caucasians and Trichoderma hook. Furthermore, the Achromobacter xylosoxidans is purchased from the China general microbiological culture Collection center with the preservation number of CGMCC 1.2998 and the original preservation date of 24 days of 4 months in 2002, the Streptomyces caucasiaticus is purchased from the China general microbiological culture collection center with the preservation number of CGMCC 4.1603 and the original preservation date of 22 days in 2001, and the Trichoderma hook is purchased from the China general microbiological culture collection center with the preservation number of CGMCC 3.13029 and the original preservation date of 26 days in 2009. Further, the preparation method of the functional microbial agent comprises the following steps: (1) Firstly preparing a bacterial suspension from freeze-dried powder of Achromobacter xylosoxidans, dripping 100 mu L of the bacterial suspension onto a nutrient gravy agar medium for culturing for 48 hours, selecting single bacterial colony for inoculating into the nutrient gravy liquid medium, culturing at 30 ℃ and 160rpm until OD 600 = 0.6 to obtain a seed solution, inoculating the seed solution into a small-sized seed tank according to the inoculation amount of 1%, and culturing at 160rpm until the bacterial concentration reaches 1X 10 9 CFU/mL to obtain a fermentation liquor of Achromobacter xylosoxidans; (2) Preparing a bacterial suspension from freeze-dried powder of streptomyces caucasianus, dripping 100 mu L of the bacterial suspension onto a first Gaoshan agar culture medi