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CN-121490134-B - Dental implant with high-bioactivity surface coating and preparation method thereof

CN121490134BCN 121490134 BCN121490134 BCN 121490134BCN-121490134-B

Abstract

The invention relates to the technical field of implants, in particular to a dental implant with a high-bioactivity surface coating and a preparation method thereof; in order to improve the bioactivity of the dental implant and improve the healing condition of the dental implant after implantation, the invention firstly carries out sand blasting roughening and acid liquor and alkali liquor soaking treatment on the surface of the dental implant, improves the hydroxyl content of the surface of the dental implant, improves the hydrophilicity of the dental implant, and simultaneously increases the active group content of the surface of the dental implant, and on the basis, the invention also introduces a polydopamine coating containing exosome microcapsules on the surface of the dental implant so as to improve the antibacterial performance of the dental implant and induce the proliferation of cells, thereby accelerating the implantation of the dental implant and greatly improving the biocompatibility of the dental implant.

Inventors

  • GAO XIAOBO
  • ZHANG YING
  • LEI BOCHENG
  • ZHAO XIAOMIN
  • Dong Tiange

Assignees

  • 赤峰市医院

Dates

Publication Date
20260508
Application Date
20260114

Claims (9)

  1. 1. The dental implant with the high-bioactivity surface coating is characterized by comprising a titanium alloy dental implant matrix and an active polydopamine coating loaded on the surface of the titanium alloy dental implant matrix; Wherein, the polydopamine coating is loaded with exosome microcapsules; the preparation method of the exosome microcapsule comprises the following steps: a. Dissolving sodium alginate in deionized water, stirring until the sodium alginate is completely dissolved, dropwise adding sodium hydroxide into the solution to adjust the pH value to 8.5-9.5, adding EDC, HCl and N-hydroxysuccinimide into the solution under a nitrogen atmosphere, stirring and mixing the solution for 30-45 min at room temperature, slowly dropwise adding dimethylaminopropylamine into the solution, stirring and reacting the solution for 18-24 h at room temperature after the dropwise adding is finished, controlling the pH value of a reaction system to be 6.5-7 in the reaction process, dialyzing the reaction solution after the reaction is finished, and freeze-drying the solution after the dialysis is finished to obtain tertiary amine modified sodium alginate; b. Dispersing tertiary amine modified sodium alginate into deionized water, regulating the pH value to 7.5-8, adding 3-chloro-2-hydroxypropyl trimethyl ammonium chloride into the deionized water, stirring and reacting for 18-24 hours, controlling the pH value to 7.5-8 in the reaction process, precipitating the product by using acetone after the reaction is finished, collecting the precipitate, and freeze-drying to obtain quaternized sodium alginate; c. Dispersing the exosomes into PBS buffer solution, uniformly mixing, adding quaternized sodium alginate and sodium alginate into the mixture, uniformly stirring and mixing, and cooling the mixture to 2-4 ℃ to obtain exosome solution; Adding tween-80 accounting for 0.3-0.5% of the volume of the liquid paraffin into the liquid paraffin, uniformly stirring and mixing, dropwise adding an exosome solution into the liquid paraffin, continuously stirring to form emulsion in the dropwise adding process, slowly dropwise adding glutaraldehyde into the emulsion, stirring and reacting for 2-4 hours at room temperature after the dropwise adding is finished, centrifugally separating and precipitating, washing the precipitate for 2-3 times by using petroleum ether, and freeze-drying to obtain the exosome microcapsule.
  2. 2. The dental implant with the high-bioactivity surface coating according to claim 1, wherein in the step a, the addition amount of each component is respectively 10 parts of sodium alginate, 5-8 parts of EDC, HCl, 3-5 parts of N-hydroxysuccinimide and 0.5-5 parts of dimethylaminopropylamine in parts by weight.
  3. 3. The dental implant with a high bioactive surface coating according to claim 1, wherein in the step a, the dialysis bag is 5-10 kDa.
  4. 4. The dental implant with the high-bioactivity surface coating according to claim 1, wherein in the step b, the addition amount of each component is respectively 10 parts of tertiary amine modified sodium alginate and 1-5 parts of 3-chloro-2-hydroxypropyl trimethyl ammonium chloride in parts by weight.
  5. 5. The dental implant with the high-bioactivity surface coating according to claim 1, wherein in the step c, the addition amount of each component is respectively 0.5-1 part of exosome, 2-10 parts of quaternized sodium alginate, 15-30 parts of sodium alginate and 1.5-5 parts of glutaraldehyde in parts by weight.
  6. 6. A method for preparing a dental implant with a high bioactive surface coating as claimed in any one of claims 1 to 5, comprising the steps of: S1, preparing a titanium alloy dental implant matrix; Cutting the surface of the titanium alloy bar into a required shape, sequentially cleaning and drying the surface by using deionized water and absolute ethyl alcohol, then carrying out sand blasting roughening treatment on the surface, then immersing the surface in acid liquor and alkali liquor, carrying out activation treatment on the surface, cleaning again by using deionized water and absolute ethyl alcohol, and drying to obtain the titanium alloy dental implant matrix; S2, preparing an active polydopamine coating; Dissolving dopamine hydrochloride in deionized water, stirring to dissolve completely, adding exosome microcapsules, continuously stirring to disperse uniformly, adding ammonia water dropwise to adjust the pH value of the mixed solution to 8.5-9, immersing the titanium alloy dental implant matrix in the mixed solution completely, heating the mixed system to 30-37 ℃, stirring to react for 6-12 h, taking out the titanium alloy dental implant matrix, washing the surface of the titanium alloy dental implant matrix with ultrapure water for 2-3 times, and freeze-drying to form an active polydopamine coating on the surface of the titanium alloy dental implant matrix to obtain the dental implant with the high-bioactivity surface coating.
  7. 7. The method for preparing a dental implant with a high bioactive surface coating according to claim 6, wherein in step S1, the acid solution is a mixed solution of sulfuric acid solution and hydrochloric acid solution in a volume ratio of 1:1; wherein the concentration of the sulfuric acid solution is 45-55wt%, and the concentration of the hydrochloric acid solution is 5-20wt%.
  8. 8. The method for preparing a dental implant with a high bioactive surface coating according to claim 6, wherein in step S1, the alkali solution is 10-20wt% sodium hydroxide solution.
  9. 9. The method for preparing a dental implant with a high-bioactivity surface coating according to claim 6, wherein in the step S2, the mass ratio of dopamine hydrochloride to exosome microcapsules is 100 (1-5).

Description

Dental implant with high-bioactivity surface coating and preparation method thereof Technical Field The invention relates to the technical field of implants, in particular to a dental implant with a high-bioactivity surface coating and a preparation method thereof. Background In the field of stomatology, dental implantation technology has increasingly become a conventional means of dental caries restoration, and the treatment effect and the life quality of patients are remarkably improved. Currently, titanium alloys are the main stream material for making implants, but they have essential differences in tissue structure from natural teeth. Particularly in the actual implantation process, the titanium alloy has low surface bioactivity, and is difficult to guide connective tissue adhesion and osteoblast proliferation, so that the postoperative healing period is often longer. In addition, the oral flora is complex, and the implant is easy to be subjected to bacterial colonization in early implantation to form a biological film, so that the difficulty of obtaining long-term stability of the implant is further increased. Disclosure of Invention The invention aims to provide a dental implant with a high-bioactivity surface coating and a preparation method thereof, so as to solve the problems in the prior art. In order to achieve the aim, the invention provides a dental implant with a high bioactivity surface coating, which is characterized by comprising a titanium alloy dental implant matrix and an active polydopamine coating loaded on the surface of the titanium alloy dental implant matrix; wherein, the polydopamine coating is loaded with exosome microcapsules. Further, the preparation method of the exosome microcapsule comprises the following steps: a. Dissolving sodium alginate in deionized water, stirring until the sodium alginate is completely dissolved, dropwise adding sodium hydroxide into the solution to adjust the pH value to 8.5-9.5, adding EDC, HCl and N-hydroxysuccinimide into the solution under a nitrogen atmosphere, stirring and mixing the solution for 30-45 min at room temperature, slowly dropwise adding dimethylaminopropylamine into the solution, stirring and reacting the solution for 18-24 h at room temperature after the dropwise adding is finished, controlling the pH value of a reaction system to be 6.5-7 in the reaction process, dialyzing the reaction solution after the reaction is finished, and freeze-drying the solution after the dialysis is finished to obtain tertiary amine modified sodium alginate; b. Dispersing tertiary amine modified sodium alginate into deionized water, regulating the pH value to 7.5-8, adding 3-chloro-2-hydroxypropyl trimethyl ammonium chloride into the deionized water, stirring and reacting for 18-24 hours, controlling the pH value to 7.5-8 in the reaction process, precipitating the product by using acetone after the reaction is finished, collecting the precipitate, and freeze-drying to obtain quaternized sodium alginate; c. Dispersing the exosomes into PBS buffer solution, uniformly mixing, adding quaternized sodium alginate and sodium alginate into the mixture, uniformly stirring and mixing, and cooling the mixture to 2-4 ℃ to obtain exosome solution; Adding tween-80 accounting for 0.3-0.5% of the volume of the liquid paraffin into the liquid paraffin, uniformly stirring and mixing, dropwise adding an exosome solution into the liquid paraffin, continuously stirring to form emulsion in the dropwise adding process, slowly dropwise adding glutaraldehyde into the emulsion, stirring and reacting for 2-4 hours at room temperature after the dropwise adding is finished, centrifugally separating and precipitating, washing the precipitate for 2-3 times by using petroleum ether, and freeze-drying to obtain the exosome microcapsule. In the step a, the addition amount of each component is respectively 10 parts of sodium alginate, 5-8 parts of EDC (electronic control unit) seed HCl, 3-5 parts of N-hydroxysuccinimide and 0.5-5 parts of dimethylaminopropylamine according to parts by weight. Further, in step a, the dialysis bag is used for dialysis at 5-10 kDa. In the step b, the addition amounts of the components are respectively 10 parts of tertiary amine modified sodium alginate and 1-5 parts of 3-chloro-2-hydroxypropyl trimethyl ammonium chloride according to parts by weight. In the step c, the addition amount of each component is respectively 0.5-1 part of exosome, 2-10 parts of quaternized sodium alginate, 15-30 parts of sodium alginate and 1.5-5 parts of glutaraldehyde according to parts by weight. Further, a method for preparing a dental implant with a high bioactive surface coating comprises the following steps: S1, preparing a titanium alloy dental implant matrix; Cutting the surface of the titanium alloy bar into a required shape, sequentially cleaning and drying the surface by using deionized water and absolute ethyl alcohol, then carrying out sand blasting roughening tre