CN-121495366-B - Probiotics-bioactive component composite membrane and preparation method and application thereof

Abstract

The invention belongs to the technical field of packaging materials, and particularly discloses a probiotic-bioactive component composite film, a preparation method and application thereof, wherein the composite film takes gelatin and carboxymethyl chitosan as a composite matrix, dandelion polyphenol and lactobacillus plantarum are loaded, and the composite film also comprises a plasticizer, wherein the plasticizer is a mixture of glycerol and polyethylene glycol. The probiotics-bioactive component composite membrane and the preparation method and application thereof are adopted, the probiotics and the bioactive component are loaded in the gelatin-carboxymethyl chitosan composite matrix together, and a stable probiotic-bioactive component-membrane material system is formed through a hydrogen bond crosslinking network of the matrix, so that the survival of lactobacillus plantarum can be effectively ensured, and the survival rate of lactobacillus plantarum is improved.

Inventors

• CHAI YANGYANG
• WEI LINGLI
• MA XINGZHU
• ZHANG MANLIN
• ZHAO MENGYUAN
• WANG XIAOYAN
• BAO YIHONG
• LI FANGFEI
• LUO JIAYUAN

Assignees

• 东北林业大学

Dates

Publication Date

20260512

Application Date

20260112

Claims (4)

1. 1. The probiotics-bioactive component composite membrane is characterized by taking gelatin and carboxymethyl chitosan as composite matrixes, loading dandelion polyphenol and lactobacillus plantarum, and further comprising a plasticizer, wherein the plasticizer is a mixture of glycerol and polyethylene glycol; the preparation method of the probiotics-bioactive component composite film comprises the following steps: Step 1, adding gelatin and carboxymethyl chitosan into deionized water according to a mass ratio, and stirring in a water bath at 60 ℃ until the gelatin and carboxymethyl chitosan are completely dissolved to obtain a mixed solution; Step 2, adding transglutaminase into the mixed solution cooled to room temperature, reacting and crosslinking for 45min at 50 ℃, then placing the solution into a water bath at 80 ℃ for 10 min, cooling to room temperature, adding dandelion polyphenol and plasticizer, and fully and uniformly stirring to prepare a film solution; And 3, inoculating the activated lactobacillus plantarum into a membrane solution, uniformly mixing, pouring the solution into a disposable plastic culture dish, and drying in a controlled incubator with the temperature of 30 ℃ and the relative humidity of 50% for 24 hours to prepare the composite membrane.
2. 2. The probiotic-bioactive ingredient composite membrane of claim 1, wherein the mass ratio of gelatin to carboxymethyl chitosan is 1:1, and the addition amount of dandelion polyphenol is 2.5% w/v.
3. 3. The probiotic-bioactive ingredient composite membrane of claim 1, wherein the mass ratio of glycerin to polyethylene glycol is 7:3, and the addition amount is 40% of the total mass of gelatin and carboxymethyl chitosan.
4. 4. The application of the probiotics-bioactive ingredient composite film as claimed in any one of claims 1-3, which is characterized by being applied to preparing meat fresh-keeping packaging films.

Description

Probiotics-bioactive component composite membrane and preparation method and application thereof Technical Field The invention relates to the technical field of packaging materials, in particular to a probiotic-bioactive component composite film, a preparation method and application thereof. Background Plastic product waste generated in the food packaging industry becomes a serious ecological threat, and meanwhile, plastic packaging materials can pollute food in the package, so that potential food safety hazards are caused. Therefore, the development of green, safe and degradable food packaging materials is a hotspot in industry research. In recent years, active food packaging films prepared by taking biomacromolecules such as polysaccharide, protein, lipid and the like as base materials are widely concerned, but single biomacromolecule base materials have the defects of insufficient mechanical properties, poor barrier property and the like, and practical application is limited. Two or more film forming base materials are blended to prepare the composite film, so that complementary advantages can be realized, and the comprehensive performance of the composite film is improved. The dandelion has the advantages of abundant resources, easy acquisition, high safety, rich active ingredients such as phenolic compounds, and the like, has various biological characteristics such as bacteriostasis, antioxidation, and the like, and the probiotics have positive effects on human health, and the generated bioactive substances can improve the stability of foods and reduce the risk of deterioration. Research shows that the co-embedding of probiotics and bioactive compounds in a film-forming substrate can improve the activity of the probiotics. The development of the composite food packaging film which is based on natural biomacromolecule matrix, cooperatively loads bioactive substances and probiotics, has excellent comprehensive performance and is simple and convenient to prepare, and has important significance for promoting the innovation development and practical application of green food packaging materials. Disclosure of Invention The invention aims to provide a probiotics-bioactive component composite film, a preparation method and application thereof, and the prepared composite film has excellent comprehensive performance, is simple to prepare, is environment-friendly, and solves the problems of poor environment friendliness, limited fresh-keeping effect and the like of the existing food packaging film. In order to achieve the aim, the invention provides a probiotics-bioactive component composite membrane, which takes gelatin and carboxymethyl chitosan as a composite matrix, loads dandelion polyphenol and lactobacillus plantarum, and also comprises a plasticizer, wherein the plasticizer is a mixture of glycerol and polyethylene glycol. Preferably, the mass ratio of the gelatin to the carboxymethyl chitosan is 1:1, and the addition amount of the dandelion polyphenol is 2.5% (w/v). Preferably, the mass ratio of the glycerol to the polyethylene glycol is 7:3, and the addition amount is 40% of the total mass of the gelatin and the carboxymethyl chitosan. The invention also provides a preparation method of the probiotics-bioactive component composite film, which comprises the following steps: Step 1, adding gelatin and carboxymethyl chitosan into deionized water according to a mass ratio, and stirring in a water bath at 60 ℃ until the gelatin and carboxymethyl chitosan are completely dissolved to obtain a mixed solution; Step 2, adding transglutaminase into the mixed solution cooled to room temperature, reacting and crosslinking for 45min at 50 ℃, then placing the solution into a water bath at 80 ℃ for 10 min, cooling to room temperature, adding dandelion polyphenol and plasticizer, and fully and uniformly stirring to prepare a film solution; And 3, inoculating the activated lactobacillus plantarum into a membrane solution, uniformly mixing, pouring the solution into a disposable plastic culture dish, and drying in a controlled incubator with the temperature of 30 ℃ and the relative humidity of 50% for 24 hours to prepare the composite membrane. The invention also provides application of the probiotics-bioactive component composite film in preparing meat fresh-keeping packaging films. The probiotics-bioactive component composite membrane and the preparation method and application thereof have the advantages and beneficial effects that: 1. The invention selects gelatin and carboxymethyl chitosan as base materials for film formation, both belong to natural macromolecules, have the characteristics of no toxicity, excellent degradation performance, good biocompatibility and the like, and are ideal green food packaging materials. However, the single gelatin film and carboxymethyl chitosan have the defects of insufficient mechanical properties and the like, and the mixed use can synergistically enhance the mechanical properties