CN-121495800-B - Non-decarboxylated Leuconostoc JXJA and application thereof

Abstract

The invention discloses a non-decarboxylated Leuconostoc JXJA and application thereof, belonging to the technical field of microbiology. Experiments prove that the fermentation filtrate of the non-decarboxylated luxuriant bacteria JXJA has a corrected mortality rate of 88.37 +/-1.16% for the two-instar larvae of the meloidogyne incognita, has a corrected mortality rate of 86.09+/-2.50% for the two-instar larvae of the meloidogyne incognita, shows that the non-decarboxylated luxuriant bacteria JXJA has a good poisoning effect on both the meloidogyne incognita and the two-instar nematodes, can remarkably inhibit the hatching rate of the oocysts of the meloidogyne incognita (the hatching rate is only 14.19+/-1.70% after being treated by the fermentation filtrate), and the comprehensive result shows that the non-decarboxylated luxuriant bacteria JXJA has an important application value for preventing and treating the nematodes in agriculture, and can be widely applied to the preparation of biological agents for preventing and treating the nematode diseases in agriculture.

Inventors

• ZHANG FAN
• YAO YINGJUAN
• XU XUELIANG
• YAO JIAN
• FAN LINJUAN
• WU CAIYUN
• LIU ZIRONG

Assignees

• 江西省农业科学院农业应用微生物研究所（江西省农村能源研究中心）

Dates

Publication Date

20260512

Application Date

20251225

Claims (8)

1. 1. A strain of non-decarboxylated luxury bacteria (LECKERCIA ADECARBOXYLATA) JXJA, wherein the non-decarboxylated luxury bacteria JXJA is deposited at the microbiological strain collection center of the university of guangdong at 8/8 of 2024, the deposit number is GDMCC No:64997, the deposit address is floor 5 of the university of 100 in the city martyr of guangdong, and the institute of microbiology of the academy of sciences of guangdong.
2. 2. The fermentation filtrate of non-decarboxylating luxuriant bacteria JXJA according to claim 1, wherein the fermentation filtrate is obtained by culturing non-decarboxylating luxuriant bacteria JXJA to obtain a bacterial suspension, and centrifuging and filtering the bacterial suspension.
3. 3. A microbial agent comprising the non-decarboxylated luxuriant bacteria JXJA according to claim 1 or the fermentation filtrate according to claim 2.
4. 4. Use of the non-decarboxylating luxuriant bacteria JXJA according to claim 1 or the fermentation filtrate according to claim 2 or the microbial inoculum according to claim 3 for controlling plant parasitic nematodes, characterized in that said plant parasitic nematodes are meloidogyne incognita and caenorhabditis elegans.
5. 5. Use of the non-decarboxylating luxuriant bacteria JXJA according to claim 1 or the fermentation filtrate according to claim 2 or the microbial inoculum according to claim 3 for the preparation of a formulation for controlling plant parasitic nematodes, characterized in that the plant parasitic nematodes are meloidogyne incognita and caenorhabditis elegans.
6. 6. The use according to claim 4 or 5, wherein said non-decarboxylating luxuriant bacteria JXJA are capable of inhibiting hatching of said meloidogyne incognita eggs.
7. 7. A formulation for controlling plant parasitic nematode disease, wherein the active ingredient of the formulation comprises the non-decarboxylated luxuriant bacteria JXJA of claim 1 or the fermentation filtrate of claim 2 or the microbial agent of claim 3; The plant parasitic nematodes are meloidogyne incognita and caenorhabditis elegans.
8. 8. A method of controlling plant parasitic nematodes comprising the step of treating said plant parasitic nematodes with a fermentation filtrate of non-decarboxylating luxurians JXJA according to claim 1; The plant parasitic nematodes are meloidogyne incognita and caenorhabditis elegans.

Description

Non-decarboxylated Leuconostoc JXJA and application thereof Technical Field The invention relates to the technical field of microbiology, in particular to non-decarboxylated luxuriant bacteria JXJA and application thereof. Background Plant-parasitic nematodes (PPNs) are an important class of economic pests that cause Plant diseases. It is estimated that economic losses in all agricultural productions due to their hazards are up to $1570 billion annually. Because the nematode is small, the nematode is difficult to directly see by naked eyes, and the yellowing symptoms caused by the nematode are similar to the water and fertilizer deficiency symptoms and are easy to ignore, so that the optimal prevention and control period is missed. For a long time, the application of chemical nematicides has been the main measure for preventing and controlling plant nematode diseases, however, the application of a large amount of chemical agents not only can cause the nematodes to generate drug resistance, but also can cause serious environmental pollution problems and harm human health, so that most of high-toxicity nematicides are forbidden or restricted. The biological control has the advantages of strong environmental compatibility, abundant available resources, contribution to the delay of the occurrence and development of the drug resistance of plant diseases and insect pests and the like. Therefore, the screening of biocontrol bacteria resources with nematode control function has important significance for developing novel biopesticide products, reducing or gradually replacing chemical pesticides to perform green control of crop nematode diseases. Disclosure of Invention The invention aims to provide a non-decarboxylated luxuriant bacteria JXJA and application thereof, which are used for solving the problems in the prior art, and the non-decarboxylated luxuriant bacteria JXJA has good poisoning effect on the meloidogyne incognita and the caenorhabditis biennis, and can obviously inhibit the hatching rate of the oocyst of the meloidogyne incognita. In order to achieve the above object, the present invention provides the following solutions: The invention provides a non-decarboxylated luxury bacteria (LECKERCIA ADECARBOXYLATA) JXJA, wherein the non-decarboxylated luxury bacteria JXJA is deposited at the microbiological strain collection center of Guangdong province at 8 months 8 of 2024, the deposition number is GDMCC No:64997, the deposition address is the building 5 No. 59 of the university of road 100 in Guangzhou City martyr, and the microbiological institute of the university of Guangdong province. The invention also provides a fermentation filtrate of the non-decarboxylated luxuriant bacteria JXJA, bacterial suspension is obtained by culturing the non-decarboxylated luxuriant bacteria JXJA, and the bacterial suspension is centrifuged and filtered to obtain the fermentation filtrate. The invention also provides a microbial agent containing the non-decarboxylated luxuriant bacteria JXJA or the fermentation filtrate. The invention also provides an application of the non-decarboxylated luxuriant bacteria JXJA or the fermentation filtrate or the microbial agent in preventing and controlling plant parasitic nematodes. The invention also provides an application of the non-decarboxylated luxuriant bacteria JXJA or the fermentation filtrate or the microbial agent in preparing a preparation for preventing and controlling plant parasitic nematodes. Optionally, the plant parasitic nematodes include meloidogyne incognita and caenorhabditis elegans. Optionally, the non-decarboxylating luxuriant bacteria JXJA is capable of inhibiting the hatching of the meloidogyne incognita eggs. The invention also provides a preparation for preventing and treating plant parasitic nematode diseases, and the active ingredients of the preparation comprise the non-decarboxylated luxuriant bacteria JXJA or the fermentation filtrate or the microbial agent. The invention also provides a method for preventing and controlling plant parasitic nematodes, which comprises the step of treating the plant parasitic nematodes by using the fermentation filtrate of the non-decarboxylated luxuriant bacteria JXJA. Optionally, the plant parasitic nematodes include meloidogyne incognita and caenorhabditis elegans. The invention discloses the following technical effects: The invention screens and identifies a strain of non-decarboxylated Lecanis (LECKERCIA ADECARBOXYLATA) JXJA with the preservation number of GDMCC No:64997. According to the test, the strain JXJA provided by the invention has the characteristics of phosphorus dissolution, potassium dissolution, nitrogen fixation and the like, IAA can be produced, the content of endocrine IAA of 7 d can reach 45.18 mg/L, plant growth can be promoted, and the test shows that the corrected mortality rate of fermentation filtrate of the non-decarboxylated luxurian bacteria JXJA on the second-instar larvae of the meloidog