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CN-121536911-B - Yanling Dan carbon dots and preparation method and application thereof

CN121536911BCN 121536911 BCN121536911 BCN 121536911BCN-121536911-B

Abstract

The invention discloses a captan carbon dot and a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method of the captan carbon dots comprises dispersing the fine powder in distilled water, stirring and pyrolyzing on a heating table, diluting the pyrolyzed product with distilled water, centrifuging to obtain supernatant, filtering with a filter membrane, dialyzing, and freeze-drying to obtain carbon dots. The average particle diameter of the obtained carbon dots is 4.51+/-1.04 nm. The captan carbon dots prepared by the invention have strong inhibitory activity on various gram-positive bacteria (such as listeria, methicillin-resistant staphylococcus aureus MRSA) and fungi (such as candida albicans), are particularly effective on clinical troublesome drug-resistant strains such as vancomycin-resistant enterococci (VRE) and MRSA, can be applied to preparation of antibacterial drugs, antibacterial dressings, disinfectants or preservatives, and have the advantages of wide raw material sources, simple preparation method, low cost, environmental friendliness and the like.

Inventors

  • ZHANG RONGPING
  • LI XINGXI
  • XIE WEIYOU
  • WANG ZHAOJIE
  • WANG QIN
  • ZHANG HENGGANG
  • CHEN XINGLONG
  • HOU BO
  • Gao Fufang
  • SU LING

Assignees

  • 云南中医药大学
  • 云南师范大学

Dates

Publication Date
20260508
Application Date
20260120

Claims (7)

  1. 1. The application of the captan carbon dots in preparing antibacterial drugs or antibacterial dressings is characterized in that the average particle size of the captan carbon dots is 4.51+/-1.04 nm, an absorption peak exists at 545nm, and green fluorescence is emitted when the excitation wavelength is 470nm, and the preparation method comprises the following steps: (1) Dispersing the pterodontoseisis fine powder in distilled water, stirring and pyrolyzing at 350+/-10 ℃ for 6-7 min to obtain a pyrolyzed product; (2) Diluting the pyrolysis product with distilled water, centrifuging to obtain supernatant, filtering with a filter membrane, dialyzing, and freeze-drying to obtain the captan carbon dot; The captan carbon dots inhibit listeria, methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci and candida albicans activity.
  2. 2. The application of the medicine according to claim 1, wherein the mass-volume ratio of the pterodontoseisis fine powder to the distilled water in the step (1) is 1 (2-3), and the unit is g/ml.
  3. 3. The application of claim 1, wherein the particle size of the pterodontoseisis fine powder in the step (1) is 80-120 meshes.
  4. 4. The method of claim 1, wherein the mass to volume ratio of the pyrolysis product to distilled water in the step (2) is 1 (2-3), and the unit is g/ml.
  5. 5. The method of claim 1, wherein the centrifugation speed in step (2) is 10000 to 12000 rpm and the centrifugation time is 28 to 32 minutes.
  6. 6. The method of claim 1, wherein the dialysis time in step (2) is 40-50 hours and the lyophilization time is 72-96 hours.
  7. 7. The method according to claim 1, wherein the filter membrane in step (2) has a pore size of 0.22. Mu.m, and the dialysis bag has a molecular weight cut-off of 3500Da.

Description

Yanling Dan carbon dots and preparation method and application thereof Technical Field The invention relates to the technical field of medicines, in particular to a captan carbon dot and a preparation method and application thereof. Background Traditional Chinese medicine resources are valuable reservoirs for finding new active molecules. The pterodontoseisis (Laggera pterodonta (DC) level) is a traditional Chinese medicine for clearing heat and detoxicating, and is widely used for treating infectious diseases such as cold, sore throat, carbuncle, swelling and sore toxin, etc. in folks. Modern pharmacological researches show that the pterodonte is rich in active ingredients such as flavonoids, sesquiterpenes, phenolic acids and the like, and has good anti-inflammatory, antiviral and antibacterial activities. However, the pterodontoseisis extract prepared by the traditional water extraction or alcohol extraction method has the limitations of complex components, low solubility of active ingredients, low bioavailability, undefined action mechanism and the like, and limits the modern development and clinical application of the pterodontoseisis extract. In recent years, the rise of nanotechnology provides a new direction for innovative research of traditional Chinese medicines. Carbon Dots (CDs) are receiving attention as an emerging Carbon-based nanomaterial due to their small size (typically less than 10 nm), good biocompatibility, low toxicity, high water solubility, ease of functionalization, and unique optical properties. Studies have shown that certain carbon dots, either by themselves or in combination, exhibit excellent antimicrobial properties, the antimicrobial mechanisms of which may include various aspects of physically destroying bacterial cell membranes, inducing Reactive Oxygen Species (ROS), and affecting bacterial metabolism. At present, the synthetic carbon sources of carbon dots are various, but single traditional Chinese medicinal materials with definite biological activity are used as precursors, and the carbon dots with both the pharmacological activity of the parent medicine and the unique properties of the nano material are prepared by a green and simple method and are used for the research of antibacterial medicines at the starting stage. At present, no report on the carbon point of the captan is found. Disclosure of Invention Aiming at the problems in the prior art, the invention develops the captan carbon dot with simple preparation process, environment protection and low cost by taking the Chinese medicine captan as a carbon source. The carbon dot has remarkable antibacterial activity on drug-resistant bacteria such as listeria, methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, candida albicans and the like, is hopeful to become a nano antibacterial agent, and provides a new strategy and means for solving the problem of bacterial drug resistance. In order to achieve the purpose of the invention, the invention provides the following technical scheme: Firstly, the invention provides an application of a captan carbon dot in preparing antibacterial drugs, antibacterial dressings, disinfectants or preservatives, which is characterized in that the captan carbon dot has an average particle size of 4.51+/-1.04 nm, has an absorption peak at 545nm, emits green fluorescence when excited with a wavelength of 470nm, and the preparation method comprises the following steps: (1) Dispersing the pterodontoseisis fine powder in distilled water, stirring and pyrolyzing at 350+/-10 ℃ to obtain a pyrolysis product; (2) Diluting the pyrolysis product with distilled water, centrifuging to obtain supernatant, filtering with a filter membrane, dialyzing, and lyophilizing to obtain the final product. Preferably, in the step (1), the mass-volume ratio of the pterodonte fine powder to the distilled water is 1:2-3, the unit is g/ml, and the pyrolysis time is 6-7 min. The CHOULINGDAN (academic name: laggera pterodonta), also known as herba Leonuri and folium Pyrolae, is plant of genus Hexagon of Compositae. The whole herb is bitter and pungent in taste and cold in nature, and has various effects of clearing heat and detoxicating, diminishing inflammation and relieving pain, eliminating phlegm and relieving cough and the like. Preferably, the particle size of the fine powder of the captan in the step (1) is 80-120 meshes. Preferably, the mass volume ratio of the pyrolysis product to distilled water in the step (2) is 1:2-3, and the unit is g/ml. Preferably, the centrifugal speed in the step (2) is 10000-12000 rpm, and the centrifugal time is 28-32 minutes. Preferably, the dialysis time in the step (2) is 40-50 hours, the drying is freeze drying, and the freeze drying time is 72-96 hours. Preferably, the filter membrane in step (2) has a pore size of 0.22 μm and the dialysis bag has a molecular weight cut-off of 3500Da. Preferably, the captan carbon spot inhibits gram positive or fungal