CN-121569760-B - Method for out-of-season reproduction of golden pomfret

Abstract

The invention discloses an out-of-season golden pomfret breeding method, and relates to the technical field of aquaculture. The method comprises the steps of selecting parents for out-of-season breeding of golden pomfret, performing ovary strengthening on the parents by using a strengthening agent, performing low-temperature heat accumulation on the parents, performing fin shearing treatment on the parents, regulating and controlling the gonad development state of the parents by using hormone, performing fertilization, obtaining fertilized eggs, incubating the fertilized eggs and the like. By strengthening, low-temperature accumulating, fin shearing and other treatments on the parent fish, the gonad development state of the parent fish is accurately regulated, the out-of-season breeding of the golden pomfret is realized, the breeding efficiency and the offspring quality of the golden pomfret are improved, the problems that the breeding of the existing golden pomfret is limited by seasons and the like are solved, and the method has good economic benefit and application prospect.

Inventors

• YANG YANG
• CAI JIANSHUN
• CHEN MENG
• CHEN ZHENNIAN
• CHEN QIONGYU
• LUO FULI
• LI XIANHUI
• CAI CHUNYOU
• CAI YOUSEN

Assignees

• 海南晨海水产有限公司

Dates

Publication Date

20260508

Application Date

20260127

Claims (5)

1. 1. A method for out-of-season reproduction of golden pomfret, comprising the steps of: (1) Selecting a parent for out-of-season reproduction of golden pomfret; (2) Jin Chang the ovaries of the female parent are in the 3-stage or more mature stage, and after the eggs are produced in 5 months, the fish body is continuously fortified, wherein the strengthening is to strengthen the ovaries of the female parent by feeding a fortified frozen fresh aquatic product, and the fortified frozen fresh aquatic product contains a fortifier, wherein the fortifier contains 5g/L vitamin E, 1g/L vitamin A, 20g/L lecithin, 10g/L bile acid, 20g/L cholesterol, 10g/L schizochytrium powder, 200g/L glycerol, 1g/L zinc chloride, 5g/L vitamin C, 2g/L arginine, 2g/L lysine, 20g/L monoglyceride and water; (3) Injecting human chorionic gonadotrophin into female fish and male fish in 5 months, wherein the injection amount of the female fish is 800-1000 units/kg, the injection amount of the male fish is 500-600 units/kg, gonad degradation is prevented, after 3-6 days of injection, the transportation is prepared, the feeding amount of the fed reinforced frozen fresh aquatic products is reduced to 0.5-1% of the weight of the fish 2 days before the transportation, and 100mg of L-carnitine is mixed in each kilogram of frozen fresh aquatic products; (4) Performing fin shearing treatment on the parent subjected to low-temperature heat accumulation; (5) The gonad development state of the parent is regulated and controlled by hormone, so that gonad maturation and ovulation are promoted; (6) Fertilize, obtain fertilized egg, fertilize egg hatch, obtain fish fry; In the step (4), the fin shearing treatment method comprises the following steps of shearing 1/3-1/2 of tail fins of the upper part or the lower part of golden pomfret, or shearing 2/3 of dorsal fins, smearing vaseline ointment containing erythromycin after fin shearing, and returning fish steaks for normal cultivation after wound healing; In the step (5), after transferring to the area with the latitude higher than 21 degrees, continuously feeding the reinforced frozen fresh aquatic product, wherein the feeding amount is 4% of the weight, the female fish feeds the reinforced frozen fresh aquatic product containing 3000-6000 units of follicle stimulating hormone per kilogram every 5 days, the male fish feeds the reinforced frozen fresh aquatic product containing 2000-2500 units of human chorionic gonadotrophin per kilogram every 5 days to promote the secondary development of gonad, the out-of-season propagation in 8-10 months is carried out in the area with the latitude higher than 21 degrees, the out-of-season propagation in 10-12 months is carried out in the area with the low latitude, the induced spawning method is as follows, the female fish and the male fish are mixed and raised, 2 mug of lutein-stimulating hormone is injected into each kilogram of body weight, 3 mug of lutein-stimulating hormone is injected into each kilogram of body after 24 hours, spawning can be carried out after 24 hours, and natural fertilization is carried out.
2. 2. The method of off-season golden pomfret propagation according to claim 1, wherein in step (1), the parent comprises a female parent selected from the group consisting of trachinotus ovatus or a hybrid thereof, the hybrid comprising a hybrid of trachinotus brucei and trachinotus ovatus, and a male parent selected from the group consisting of trachinotus ovatus.
3. 3. The method of off-season golden pomfret propagation according to claim 1 wherein in step (1) the parent is selected to be more than 4 years old and the gonad develops to a healthy fish after stage 3.
4. 4. The method for out-of-season propagation of golden pomfret according to claim 1, wherein in the step (2), the preparation method of the reinforced fresh aquatic product is that every 100L of the reinforcing agent is mixed with 100g of fish meal, every 200g of the mixture is mixed with 10 kg of fresh aquatic product, the fresh aquatic product comprises squid and bonito, the mass ratio of the squid to the bonito is 1:3, and the feeding amount of the reinforced fresh aquatic product is 2% -5% of the weight of the fish.
5. 5. The method for out-of-season reproduction of golden pomfret according to claim 1, wherein in the step (6), fertilization rate is improved by adopting a method that a hatching barrel of 1 ton is used for treating fertilized eggs of 2-5 kg, natural seawater is added into the hatching barrel, 100g of active carbon with an iodine value of 500-1000 is added into the hatching barrel according to each ton of water, sodium hypochlorite is added into the hatching barrel according to a final concentration of 0.1ppm, hatching temperature is 24-30 ℃, salinity is 33-36 per mill, and dead eggs are discharged after one hour after the blastula period, gastrula period, sarcomere formation period and heart beating period are finished respectively.

Description

Method for out-of-season reproduction of golden pomfret Technical Field The invention belongs to the technical field of aquaculture, and particularly relates to an out-of-season reproduction method for golden pomfret. Background Jin Chang belonging to the genus Trachinotus (Trachinotus) belonging to the family Trachinotus (Perciformes) belonging to the family Trachinotus (CARANGIDAE), which is a warm water type middle-upper-layer migratory fish. The fish fin has flat shape, oval shape, silvery white scales coated on the surface, and golden color of the fish fin and tail. Jin Chang adult fish body can reach 50 cm, and the maximum individual weight exceeds 23 kg. Geographically distributed, the species mainly inhabits tropical and subtropical warm water sea areas such as the western Pacific ocean and the Indian ocean, and adapts to the ecological environment of the warm sea areas. Golden pomfret is also an important seawater economic fish in China, the annual yield exceeds 30 ten thousand tons, and the golden pomfret is the largest seawater cultured fish species in China. Golden pomfret is mainly cultivated on large scale in coastal areas of China south China (Guangdong, guangxi, hainan), southeast Asia and the like, and has the advantages of short cultivation period, remarkable economic benefit and stable market demand. Jin Chang has tender and compact meat quality, small flavor change after freezing, less intramuscular thorns, high protein content, low fat content, and rich unsaturated fatty acid and minerals such as calcium, phosphorus, and the like, and has edible value and nutritional value. The breeding season of golden pomfret is 2-7 months per year, and the period provides all fries for the whole industry. However, in the golden pomfret cultivation process, extreme weather or diseases such as typhoons are frequently encountered, so that cultivated golden pomfret is subjected to large-scale loss, seedling supplement is needed, partial farmers purchase autumn and winter seedlings to live on the market earlier to obtain higher benefit, and in addition, the temperature of the southeast Asian nations in the whole year is suitable, and the temperature ranges suitable for survival of golden pomfret are also expected to have stable seedling supply. Based on the above requirements, development work of the anti-season fingerlings of golden pomfret is necessary. At present, although a fish out-of-season breeding method has been developed, the out-of-season breeding of golden pomfret has not been reported in detail. As a warm water upper layer migration fish, the golden pomfret has unique biological characteristics and ecological requirements, and the existing out-of-season breeding method of other fishes cannot be completely suitable for golden pomfret. Therefore, there is an urgent real need to develop an efficient and easy to operate off-season breeding method specifically adapted for golden pomfret. Disclosure of Invention In view of the defects of the prior art, the invention aims to provide the method for out-of-season breeding of the golden pomfret, break the season limit of breeding of the golden pomfret, improve the breeding efficiency and the offspring seed quality of the golden pomfret, realize the stable supply of the golden pomfret and provide important support for the development of the golden pomfret industry. The technical scheme of the invention mainly comprises the following contents: an out-of-season golden pomfret propagation method comprises the following steps: (1) Selecting a parent for out-of-season reproduction of golden pomfret; (2) Carrying out ovary strengthening on the female parent by feeding the aquatic products containing the strengthening ice; (3) Carrying out low-temperature accumulation on the parent by using north-south transportation; (4) Performing fin shearing treatment on the parent subjected to low-temperature heat accumulation; (5) The gonad development state of the parent is regulated and controlled by hormone, so that gonad maturation and ovulation are promoted; (6) Fertilize, obtain fertilized egg, fertilize egg hatch, obtain fish fry. Preferably, in the step (1), the parent comprises a female parent selected from trachinotus ovatus or a hybrid thereof, the hybrid comprises a hybrid of trachinotus brothers Trachinotus blochii and trachinotus ovatus Trachinotus ovatus, and the male parent comprises trachinotus ovatus. Preferably, in the step (1), the parent is selected from healthy fish bodies with normal body state, age 4 or more and gonad development to 3 or more mature stage. Preferably, in the step (2), in the Hainan region, after the spawning of the fish body is completed in 2-5 months, the ovary reinforcement is required to be continuously performed, so that conditions are created for subsequent out-of-season propagation. Preferably, the fortified frozen fresh aquatic product contains a fortifier, wherein the fortifier contains 5g/L vitamin E, 1g/L vitamin A, 20g/