CN-121570590-B - Composite material for treating periodontitis based on photodynamic force and preparation method and application thereof

Abstract

The application belongs to the technical field of preparation of medical materials, and particularly relates to a composite material for treating periodontitis based on photodynamic, and a preparation method and application thereof. Dispersing sodium dodecyl benzene sulfonate, tin chloride pentahydrate and L-cysteine in a mixed solution of water and glycol, uniformly stirring, performing hydrothermal reaction to obtain nano sheets, then mixing with sulfhydryl-polyethylene glycol, performing ultrasonic treatment to obtain tin disulfide nano sheets, mixing germanium oxide solution, chromium nitrate nonahydrate solution, zinc nitrate hexahydrate, gallium nitrate hydrate and water, performing light-shielding stirring, adding ammonia water, continuously stirring uniformly, performing hydrothermal reaction to obtain long-light-emitting nano particles, finally dissolving the tin disulfide nano sheets and the long-light-emitting nano particles in water, performing light-shielding stirring, adding methylene blue solution, and continuously performing light-shielding stirring to obtain the light-emitting nano particles. The composite material based on photodynamic therapy periodontitis prepared by the application can effectively improve the curative effect of photodynamic therapy in periodontitis treatment.

Inventors

• SONG LI
• HU SHURUI
• XU FANCHENG
• DAI FANG
• XU TING
• DENG TIAN
• SONG CHAORU
• WU JIANXIN
• WANG ZIXUAN
• HUANG TIANYU

Assignees

• 南昌大学第二附属医院

Dates

Publication Date

20260505

Application Date

20260120

Claims (8)

1. 1. The preparation method of the composite material for treating periodontitis based on photodynamic therapy is characterized by comprising the following steps of: dispersing sodium dodecyl benzene sulfonate, stannic chloride pentahydrate and L-cysteine in a mixed solution of water and glycol, uniformly stirring, performing a first hydrothermal reaction, centrifuging after the reaction is finished, washing, and freeze-drying to obtain a nano sheet; Mixing the nanosheets with mercapto-polyethylene glycol, placing the mixture in water for ultrasonic treatment, stirring, centrifuging, washing, and freeze-drying to obtain tin disulfide nanosheets; Dispersing germanium oxide in water, and then dropwise adding ammonia water until the germanium oxide is completely dissolved to obtain germanium oxide solution; Dissolving chromium nitrate nonahydrate in water to obtain a chromium nitrate nonahydrate solution; Mixing germanium oxide solution, chromium nitrate nonahydrate solution, zinc nitrate hexahydrate, gallium nitrate hydrate and water, stirring in a dark place, adding ammonia water, continuously stirring uniformly, performing a second hydrothermal reaction, centrifuging, washing, freeze-drying, and calcining to obtain long luminescent nano particles; dissolving the tin disulfide nanosheets and the long luminescent nanoparticles in water, stirring in a dark place, then adding a methylene blue solution, continuously stirring in a dark place, centrifuging, washing, and freeze-drying to obtain the composite material for treating periodontitis based on photodynamic therapy; The dosage ratio of the sodium dodecyl benzene sulfonate, the stannic chloride pentahydrate and the L-cysteine is 0.418g to 0.1754g to 0.4846g; The mass ratio of the nano-sheet to the sulfhydryl-polyethylene glycol is 5:1; the mass ratio of the tin disulfide nano-sheet to the long luminescent nano-particles is 1:2.
2. 2. The preparation method according to claim 1, wherein the temperature of the first hydrothermal reaction is 150 ℃ to 200 ℃ and the time of the first hydrothermal reaction is 8h to 12h.
3. 3. The preparation method according to claim 1, wherein the dosage ratio of germanium oxide solution, chromium nitrate nonahydrate solution, zinc nitrate hexahydrate and gallium nitrate hydrate is 400 μl:752 μl:1.2mmol:1.6mmol; the concentration of the germanium oxide solution is 0.5mmol/mL, and the concentration of the chromium nitrate nonahydrate solution is 0.01mmol/mL.
4. 4. The preparation method according to claim 1, wherein the volume ratio of water to glycol in the water and glycol mixture is 1:1; The calcination temperature was 800 ℃ and the calcination time was 1h.
5. 5. The preparation method according to claim 1, wherein the temperature of the second hydrothermal reaction is 220 ℃, and the time of the second hydrothermal reaction is 6 hours.
6. 6. The method of claim 1, wherein the methylene blue solution has a concentration of 100 μg/mL.
7. 7. A composite material for treating periodontitis based on photodynamic therapy, characterized in that it is prepared by the preparation method according to any one of claims 1 to 6.
8. 8. The use of the photodynamic therapy-based composite material according to claim 7 for the preparation of an antibacterial material.

Description

Composite material for treating periodontitis based on photodynamic force and preparation method and application thereof Technical Field The application belongs to the technical field of preparation of medical materials, and particularly relates to a composite material for treating periodontitis based on photodynamic, and a preparation method and application thereof. Background Periodontitis is the leading cause of loss of teeth in adults in China, and affects not only the local health of the oral cavity, but also the risk induction and aggravation factors of various diseases of the whole body. Photodynamic therapy is to irradiate a photosensitizer with light of a specific wavelength to excite the photosensitizer to generate active oxygen substances such as singlet oxygen and the like, thereby killing and destroying pathogenic microorganisms and pathological cells. Photodynamic therapy is safe, effective and minimally invasive and is currently used for the clinical treatment of periodontitis. However, the photodynamic therapy system in the current research and clinical use has the defects of three factors of photodynamic (photosensitizer, excitation light and oxygen) that the photosensitizer is easy to self-aggregate to influence the photosensitization reaction, the penetration depth of the tissue of the excitation light is shallow to influence the photoexcitation efficiency and the oxygen supply is insufficient to influence the yield of active oxygen. The above-mentioned disadvantages may reduce the therapeutic effect of photodynamic therapy in periodontitis and limit the further application of photodynamic therapy in other disease areas, such as tumor treatment. Disclosure of Invention The invention aims to solve the defects of the prior art, and mainly aims to solve the problems that a photodynamic therapy system in the prior art has defects in three photodynamic factors (photosensitizer, excitation light and oxygen), wherein the photosensitizer is easy to self-gather to influence a photosensitive reaction, the penetration depth of an excited light tissue is shallow to influence the light excitation efficiency, and the oxygen supply is insufficient to influence the yield of active oxygen, and particularly provides a composite material based on photodynamic therapy periodontitis, a preparation method and application thereof, and a technical scheme is adopted as follows: in a first aspect, the invention provides a method for preparing a composite material for treating periodontitis based on photodynamic therapy, which comprises the following steps: Dispersing sodium dodecyl benzene sulfonate, stannic chloride pentahydrate and L-cysteine in a mixed solution of water and glycol, uniformly stirring, performing a first hydrothermal reaction, centrifuging after the reaction is finished, washing, and freeze-drying to obtain a nano sheet; Mixing the nanosheets with mercapto-polyethylene glycol, placing the mixture in water for ultrasonic treatment, stirring, centrifuging, washing, and freeze-drying to obtain tin disulfide nanosheets; Dispersing germanium oxide in water, and then dropwise adding ammonia water until the germanium oxide is completely dissolved to obtain germanium oxide solution; Dissolving chromium nitrate nonahydrate in water to obtain a chromium nitrate nonahydrate solution; Mixing germanium oxide solution, chromium nitrate nonahydrate solution, zinc nitrate hexahydrate, gallium nitrate hydrate and water, stirring in a dark place, adding ammonia water, continuously stirring uniformly, performing a second hydrothermal reaction, centrifuging, washing, freeze-drying, and calcining to obtain long luminescent nano particles; and dissolving the tin disulfide nanosheets and the long luminescent nanoparticles in water, stirring in a dark place, then adding a methylene blue solution, continuously stirring in a dark place, centrifuging, washing, and freeze-drying to obtain the composite material for treating periodontitis based on photodynamic therapy. As a further preferred embodiment, the sodium dodecyl benzene sulfonate, tin chloride pentahydrate and L-cysteine are used in a ratio of 0.418g to 0.1754g to 0.4846g. As a further preferred embodiment, the temperature of the first hydrothermal reaction is 150 ℃ to 200 ℃ and the time of the first hydrothermal reaction is 8h to 12h. As a further preferred embodiment, the mass ratio of the nanoplatelets to the thiol-polyethylene glycol is 5:1. As a further preferable embodiment, the dosage ratio of germanium oxide solution, chromium nitrate nonahydrate solution, zinc nitrate hexahydrate and gallium nitrate hydrate is 400. Mu.L, 752. Mu.L, 1.2mmol, 1.6mmol, the concentration of germanium oxide solution is 0.5mmol/mL, and the concentration of chromium nitrate nonahydrate solution is 0.01mmol/mL. As a further preferable embodiment, the volume ratio of water to glycol in the water and glycol mixed solution is 1:1; The calcination temperature was 800 ℃ and the calcin