CN-121574220-B - CtBBX4 protein and gene related to plant salt tolerance and application thereof

Abstract

The invention belongs to the technical field of genetic engineering, and particularly relates to CtBBX protein and gene related to plant salt tolerance and application thereof. The invention provides CtBBX protein related to plant salt tolerance, and the amino acid sequence of the CtBBX protein is shown as SEQ ID NO. 1. According to the CtBBX protein disclosed by the invention, the salt tolerance of plants can be improved, and the results of examples show that the CtBBX gene is overexpressed in plants to improve the germination rate of the plants and the biomass, the salt tolerance of the plants is improved by improving the oxidation resistance of the plants, the CtBBX gene is constructed into a plant expression vector, and genetic transformation is carried out by an agrobacterium-mediated genetic transformation method, so that new germplasm with the salt tolerance characteristic can be effectively obtained, and the method has important significance in developing salt-tolerant crop breeding.

Inventors

• YANG ZHONGREN
• Lv yue
• Fan Shuwang
• Chang Xiaoyao
• ZHANG FENGLAN
• ZHANG XIAOYAN
• ZHANG DONG
• HUANG XIUMEI
• CHEN GUIHUA
• YANG YAN
• CUI XIAOLONG

Assignees

• 内蒙古农业大学

Dates

Publication Date

20260505

Application Date

20260128

Claims (10)

1. 1. A CtBBX protein related to plant salt tolerance, wherein the amino acid sequence of the CtBBX protein is shown as SEQ ID NO. 1.
2. 2. A CtBBX gene related to plant salt tolerance, wherein the nucleotide sequence of the CtBBX gene is shown as SEQ ID NO. 2.
3. 3. Use of CtBBX protein according to claim 1 or CtBBX gene according to claim 2 for regulating salt tolerance in plants, said plants being cucumis sativus and/or arabidopsis thaliana.
4. 4. The method of claim 3, wherein the modulation comprises increasing CtBBX protein content or increasing CtBBX gene expression to increase salt tolerance in the plant.
5. 5. The use according to claim 4, wherein the improvement of salt tolerance of the plant comprises at least one of (1) improvement of germination rate, (2) improvement of plant growth amount, and (3) improvement of antioxidant capacity.
6. 6. A recombinant expression vector, which is characterized in that, the recombinant expression vector comprises the CtBBX gene according to claim 2.
7. 7. A recombinant bacterium comprising the recombinant expression vector of claim 6.
8. 8. Use of CtBBX protein 3834 according to claim 1, ctBBX gene according to claim 2, recombinant expression vector according to claim 6 or recombinant bacterium according to claim 7 for the cultivation of salt tolerant plant varieties, said plants being cucumis sativus and/or arabidopsis thaliana.
9. 9. A method for improving salt tolerance of a plant, which is characterized by comprising the step of transferring the recombinant expression vector of claim 6 or the recombinant bacterium of claim 7 into a plant, wherein the plant is a ground tip melon and/or an arabidopsis thaliana.
10. 10. A transgenic plant is characterized in that the transgenic plant overexpresses CtBBX genes, the nucleotide sequence of the CtBBX genes is shown as SEQ ID NO.2, and the plant is ground tip melon and/or Arabidopsis thaliana.

Description

CtBBX4 protein and gene related to plant salt tolerance and application thereof Technical Field The invention belongs to the technical field of genetic engineering, and particularly relates to CtBBX protein and gene related to plant salt tolerance and application thereof. Background Soil salinization has become a major threat to global agricultural ecology, and excessive salinity damages plant metabolic balance through osmotic stress and ion poisoning, so that crop growth is blocked, yield is reduced sharply, and saline-alkali soil development and utilization are severely restricted. The functional genes of wild salt-tolerant plants are mined, and the functional genes are core ways for cultivating salt-tolerant crops, such as salt-tolerant genes of wild plants like sesbania and the like, and are successfully applied to crop improvement. The ground tip melon is used as a native plant in arid and saline-alkali areas in the north of China, has obvious salt tolerance characteristics, however, the molecular mechanism of salt tolerance of the ground tip melon is not deeply researched at present, and the separation and identification and functional verification of salt tolerance key genes in the genome are still blank. Therefore, the salt-tolerant genes are screened from the ground tip melons, so that the plant salt-tolerant gene resource library can be enriched, a new gene target can be provided for saline-alkali soil crop breeding, and the method has important theoretical significance and application prospect. Disclosure of Invention The invention aims to provide CtBBX protein and gene related to plant salt tolerance and application thereof, wherein the CtBBX protein can improve the plant salt tolerance and provide a new path for cultivating new salt tolerance germplasm. The invention provides CtBBX protein related to plant salt tolerance, and the amino acid sequence of the CtBBX protein is shown as SEQ ID NO. 1. The invention also provides CtBBX gene related to plant salt tolerance, and the nucleotide sequence of the CtBBX gene is shown as SEQ ID NO. 2. The invention also provides an application of CtBBX protein or CtBBX gene in regulating and controlling plant salt tolerance. As a preferred embodiment, the regulation comprises increasing CtBBX4 protein content or increasing CtBBX gene expression level to increase salt tolerance of the plant. As a preferable scheme, the improvement of the salt tolerance of the plant comprises at least one of (1) improvement of germination rate, (2) improvement of plant growth amount and (3) improvement of antioxidation capability. As a preferred embodiment, the plant comprises a. Tumefaciens and/or arabidopsis thaliana. The invention also provides a recombinant expression vector which comprises CtBBX genes according to the scheme. The invention also provides a recombinant bacterium which comprises the recombinant expression vector according to the scheme. The invention also provides an application of CtBBX protein, ctBBX gene, recombinant expression vector or recombinant bacteria in cultivating salt-tolerant plant varieties. The invention also provides a method for improving the salt tolerance of plants, which comprises the following steps of transferring the recombinant expression vector or the recombinant bacteria in the scheme into plants. The invention provides CtBBX protein related to plant salt tolerance, and the amino acid sequence of the CtBBX protein is shown as SEQ ID NO. 1. According to the CtBBX protein disclosed by the invention, the salt tolerance of plants can be improved, and the results of examples show that the CtBBX gene is overexpressed in plants to improve the germination rate of the plants and the biomass, the salt tolerance of the plants is improved by improving the oxidation resistance of the plants, the CtBBX gene is constructed into a plant expression vector, and genetic transformation is carried out by an agrobacterium-mediated genetic transformation method, so that new germplasm with the salt tolerance characteristic can be effectively obtained, and the method has important significance in developing salt-tolerant crop breeding. Drawings In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments will be briefly described below. FIG. 1 is a subcellular localization map of the FIG. CtBBX gene of Calotropis gigantea; FIG. 2 is a graph showing transcriptional activation activity of the gene CtBBX A of Calotropis gigantea; FIG. 3 is a diagram showing the functional verification result of the yeast overexpressed by the gene CtBBX of Calotropis gigantea, wherein A, B is a diagram showing the growth vigor and the concentration OD 600 of the yeast overexpressed and the yeast empty vector on a culture medium under normal conditions, C, D is a diagram showing the growth vigor and the concentration OD 600 of the yeast overexpressed and the