CN-121574966-B - Protease for improving resistance of potatoes to late blight, coding gene and application

Abstract

The invention discloses protease for improving resistance of potatoes to late blight, a coding gene and application thereof, belonging to the technical fields of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD is shown as SEQ ID NO.2 or a derivative sequence with the same disease resistance function. The nucleotide sequence of the coding gene is shown as SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. By introducing StRD gene into potato and over-expressing it, the resistance of potato to late blight can be significantly enhanced. The invention provides effective gene resources and biotechnology means for cultivating new disease-resistant potato varieties.

Inventors

• FANG HONG
• MU JIAYU
• TENG LINLIN
• YANG XIANGXUAN
• ZHU YALING

Assignees

• 云南师范大学

Dates

Publication Date

20260508

Application Date

20260127

Claims (2)

1. 1. Use of protease StRD, a gene encoding protease StRD21 or a recombinant expression vector comprising a gene encoding protease StRD21 for growing potatoes resistant to late blight, wherein the use is achieved by overexpressing protease StRD21 in potatoes, wherein the amino acid sequence of protease StRD is shown in SEQ ID No.2 and the nucleotide sequence of the gene encoding protease StRD21 is shown in SEQ ID No. 1.
2. 2. Use of protease StRD, a gene encoding protease StRD21 or a recombinant expression vector comprising a gene encoding protease StRD21 for increasing resistance of a potato to late blight, wherein the use is achieved by overexpressing protease StRD21 in the potato, wherein the amino acid sequence of protease StRD21 is as shown in SEQ ID No.2, and the nucleotide sequence of the gene encoding protease StRD21 is as shown in SEQ ID No. 1.

Description

Protease for improving resistance of potatoes to late blight, coding gene and application Technical Field The invention belongs to the technical field of genetic engineering, and particularly relates to protease for improving resistance of potatoes to late blight, and a coding gene and application thereof. Background Potatoes are globally important crops such as grains, vegetables and feeds, and the stability and safety of the yield of the potatoes are of great significance for guaranteeing the global grain safety. However, potato production is severely threatened by late blight caused by phytophthora infestans throughout the year. The disease has the characteristics of strong outbreak, high transmission speed, serious hazard degree and the like, and can lead to dead stems and leaves of potatoes and rotted tubers in a short period of time, thereby causing destructive yield loss. Historically, the well-known "Irish macrofamine" is a serious consequence of potato late blight. To date, late blight has been considered a "first killer" in potato production. At present, the prevention and treatment of potato late blight mainly depend on chemical pesticides. However, the long-term use of a large amount of chemical agents not only increases the production cost, leads to the generation of drug resistance of pathogenic bacteria, but also causes the problems of environmental pollution, pesticide residues and the like, and is contrary to the agricultural sustainable development concept. Therefore, breeding and utilizing disease resistant varieties is recognized as the most economical, effective and environmentally friendly strategy for controlling late blight. In the long-term co-evolution process of plants and pathogenic microorganisms, a double-layered defense system is formed to resist pathogen infection. For a long time, the plant immune system has been largely divided into two classes (1) plants trigger the first layer immune system (PTI) of plants against infection by the pathogen through direct recognition of pathogen-associated molecular patterns (PAMPs) of the pathogen by cell membrane-localized Pattern Recognition Receptors (PRRs). (2) Pathogenic bacteria can endocrine effector into plant cells, so that the immune system of the plant is attacked to further infect the plant, and the plant evolves NBS-LRR disease resistance proteins to directly or indirectly recognize the effector of the pathogenic bacteria, so that the secondary immune system (effector-TRIGGERED IMMUNITY, ETI) of the plant is triggered, and a stronger immune response is activated to resist the infection of the pathogenic bacteria. Potato late blight-resistant breeding relies primarily on the mining and utilization of disease-resistant genes from wild or cultivated germplasm resources. Traditional breeding has achieved some success by polymerizing known NBS-LRR disease resistance genes in cultivars. However, this resistance mechanism, while highly effective, tends to be of small specificity and is prone to rapid loss of resistance due to mutation of the phytophthora infestans population or the generation of new species, and it is difficult to provide durable, stable protection in production. In order to obtain durable and broad-spectrum resistance, it is becoming a research hotspot to mine new gene families with broad-spectrum disease resistance potential. PTI immunity is independent of the defending path of NBS-LRR disease resistance genes, has no species specificity in targeting pathogenic bacteria, and has the characteristic of broad-spectrum disease resistance. Among them, cysteine proteases play an important role in the plant PTI immune response, but their specific functions and development applications in potato anti-late blight remain small. Thus, there is an urgent need in the art to identify new, specifically functional genes associated with resistance to potato late blight, particularly those genes that may be involved in the underlying immune pathway, with the potential to provide durable resistance. The function of the strain is verified by a genetic engineering means and the strain is applied to potato breeding practice, so that a brand new genetic resource and an effective way are provided for solving the problem of potato late blight control and cultivating excellent new varieties with lasting resistance. Disclosure of Invention The invention aims to provide protease StRD which can effectively improve the resistance of potatoes to late blight, and a coding gene and application thereof. In order to achieve the above purpose, the present invention adopts the following technical scheme: In a first aspect, the present invention provides a protease StRD for increasing resistance of potato to late blight, which is any one of the following a) -b): a) A protease consisting of an amino acid sequence as shown in SEQ ID NO. 2; b) And (3) the derivative protein which is obtained by substituting, deleting or inserting 1-20 (such as 1,2, 3, 5,