CN-121592557-B - Pseudomonas and application thereof in degradation of octyl p-methoxycinnamate

Abstract

The invention discloses a strain of octyl p-methoxycinnamate degrading bacteria. Pseudomonas sp. OMC5 with the information of the collection of microorganisms from Guangdong province, collection center (GDMCC), collection date of 2025, 11 and 25 days, collection address of Guangzhou, martyr, 30 th floor, 5 th Guangdong province, microorganism institute, post code of 510070, collection number of GDMCC, 67353. The invention separates a Pseudomonas sp OMC5 strain with good degradation effect on octyl p-methoxycinnamate from sludge of Guangzhou sewage treatment plant, and can be used for degradation and removal of octyl p-methoxycinnamate. Provides an economic and green method for removing the novel organic pollutants.

Inventors

• LIU YOUSHENG
• CHEN YANFEN
• TANG QINGLIN
• ZHANG JINNA
• HU LIXIN

Assignees

• 华南师范大学

Dates

Publication Date

20260508

Application Date

20260121

Claims (6)

1. 1. Pseudomonas sp. OMC5, accession number GDMCC No.67353.
2. 2. A microbial agent comprising the Pseudomonas OMC5 or a fermentation broth thereof according to claim 1 as an active ingredient.
3. 3. Use of pseudomonas OMC5 according to claim 1 or a microbial agent according to claim 2 for degrading octyl p-methoxycinnamate.
4. 4. The use according to claim 3, wherein the octyl p-methoxycinnamate is octyl p-methoxycinnamate in a soil or water environment.
5. 5. A method for degrading octyl p-methoxycinnamate, which is characterized in that pseudomonas OMC5 according to claim 1 or a microbial agent according to claim 2 is inoculated into an environment containing octyl p-methoxycinnamate, and the octyl p-methoxycinnamate is degraded by pseudomonas OMC 5.
6. 6. The method of claim 5, wherein the octyl p-methoxycinnamate is octyl p-methoxycinnamate in a soil or water environment.

Description

Pseudomonas and application thereof in degradation of octyl p-methoxycinnamate Technical Field The invention belongs to the field of sewage treatment, and particularly relates to octyl p-methoxycinnamate degrading bacteria. Background Octyl p-methoxycinnamate is one of the commonly used organic ultraviolet absorbers. It is widely added to various personal care products such as cosmetics and sunscreens to protect the skin from sunlight and other consumer products such as plastics, textiles, food packaging, tires and cleaners to prevent photodegradation. Recreational activities such as swimming or wastewater treatment plants are frequently detected in the environment due to their insufficient removal capacity. Because of its high lipophilicity (logKow > 4), octyl p-methoxycinnamate entering an aqueous environment tends to accumulate in organisms and has biological amplification potential. Octyl p-methoxycinnamate accumulated for a long time can interfere with endocrine systems of organisms, seriously threaten their development and reproduction, for example, octyl p-methoxycinnamate can reduce thyroid hormones of adult males and larval zebra fish, and can also influence transcription of juvenile neurotoxicity related genes and adult male fish nephrotoxicity related genes （Seoyoon Chu, Ba Reum Kwon, Young-Min Lee, Kyung-Duk Zoh, Kyungho Choi, Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio), Chemosphere, Volume 263, 2021, 128176）. Currently, there is limited research on octyl p-methoxycinnamate removal. Although the sewage treatment plant has a certain removal effect on octyl p-methoxycinnamate, organic ultraviolet absorbers such as octyl p-methoxycinnamate and the like are frequently detected in the environment, which indicates that a method for removing the novel organic pollutants in a targeted manner is lacking. Considering both environmental and economic factors, microbial degradation is considered to be an economical and green method for removing organic matters in the environment at present. There is limited research on bacterial strain degradation of octyl p-methoxycinnamate at home and abroad, and related research is needed to be carried out. Disclosure of Invention The invention aims to provide a strain of octyl p-methoxycinnamate degrading bacteria. The octyl p-methoxycinnamate degrading bacterium is pseudomonas OMC5, and the preservation information comprises a preservation unit, namely Guangdong province microorganism strain preservation center (GDMCC), a preservation date, namely 2025, 11 months and 25 days, a preservation address, namely Guangzhou city martyr, namely Guangzhou university No. 100, no. 59 building 5, guangdong province microorganism research institute, a postal code, namely 510070, and a preservation number, namely GDMCC No.67353. The invention also provides a microbial agent which contains the pseudomonas OMC5 or fermentation liquor thereof as an active ingredient. Preferably, the microbial agent may be a microbial additive. The invention also provides application of the pseudomonas OMC5 or the microbial agent in degrading octyl p-methoxycinnamate. Preferably, the octyl p-methoxycinnamate comprises octyl p-methoxycinnamate in various environments, such as soil and water environments. The invention also provides a method for degrading octyl p-methoxycinnamate, which comprises the steps of inoculating the pseudomonas OMC5 or the microbial agent into an environment containing octyl p-methoxycinnamate, and degrading the octyl p-methoxycinnamate by using the pseudomonas OMC 5. The invention separates a pseudomonas (Pseudomonas sp.) OMC5 strain with good degradation effect on octyl p-methoxycinnamate from sludge of Guangzhou sewage treatment plant, and can be used for degradation and removal of octyl p-methoxycinnamate. Provides an economic and green method for removing the novel organic pollutants. Pseudomonasp.omc5, its preservation information is the collection unit of Guangdong province microorganism strain (GDMCC), the preservation date is 2025, 11 and 25 days, the preservation address is Guangzhou city martyr, 30 th floor, 5 th Guangdong province microorganism research institute, post code 510070, and the preservation number is GDMCC No.67353. Drawings FIG. 1 is a colony morphology of Pseudomonas OMC 5; FIG. 2 is a graph showing the effect of Pseudomonas OMC5 on the degradation of octyl p-methoxycinnamate. Detailed Description The following examples are further illustrative of the invention and are not intended to be limiting thereof. Example 1 domestication, isolation and identification of octyl p-methoxycinnamate degrading bacteria OMC Bacterial sources are collected from sludge of a Guangzhou sewage treatment plant, 30mL of sludge is inoculated into a 250mL conical flask filled with 120mL of modified MSM liquid culture medium, the modified MS