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CN-121610410-B - Flexibacterium strain and application thereof in degrading sulfachloropyridazine

CN121610410BCN 121610410 BCN121610410 BCN 121610410BCN-121610410-B

Abstract

The invention discloses a campylobacter strain and application thereof in degrading sulfachloropyridazine. The buckeye (Ancylobacter sp.) SAs-18 has the preservation information of the collection unit of Guangdong province microorganism strain collection (GDMCC), the preservation date of 2025, 11 and 25 days, the preservation address of Guangzhou city martyr, 30 th university, 59 th building, 5 th Guangdong province microorganism research institute, post code of 510070 and the preservation number of GDMCC No.67354. The invention separates the campylobacter (Ancylobacter sp.) SAs-18 with good sulfachloropyridazine degradation effect from the surface soil of a heavily polluted area of a wastewater discharge site of a pharmaceutical factory in the bergamot of Guangdong province, and can be used for degrading and removing sulfachloropyridazine. Provides an economic and green method for removing the novel organic pollutants.

Inventors

  • LIU YOUSHENG
  • ZHU AIQIN
  • CHEN YANFEN
  • WEN ZHUO
  • TANG ZHIJIE
  • ZHANG JINNA
  • HU LIXIN

Assignees

  • 华南师范大学

Dates

Publication Date
20260508
Application Date
20260121

Claims (7)

  1. 1. Campylobacter (Ancylobacter sp.) SAs-18, accession number GDMCC No.67354.
  2. 2. A microbial agent comprising the campylobacter SAs-18 or a fermentation liquid thereof according to claim 1 as an active ingredient.
  3. 3. The microbial agent of claim 2, wherein the microbial agent is a microbial additive.
  4. 4. Use of the campylobacter SAs-18 of claim 1 or the microbial agent of claim 2 for degrading sulfachloropyridazine.
  5. 5. The use according to claim 4, wherein the sulfachloropyridazine is sulfachloropyridazine in soil, water environments.
  6. 6. A method for degrading sulfachloropyridazine, which is characterized in that the campylobacter SAs-18 of claim 1 or the microbial agent of claim 2 is inoculated into an environment containing sulfachloropyridazine, and the sulfachloropyridazine is degraded by using the campylobacter SAs-18.
  7. 7. The method according to claim 6, wherein the sulfachloropyridazine is sulfachloropyridazine in soil and water environments.

Description

Flexibacterium strain and application thereof in degrading sulfachloropyridazine Technical Field The invention belongs to the field of sewage treatment, and particularly relates to sulfachloropyridazine degrading bacteria and application thereof. Background The sulfonamide antibiotics are broad-spectrum antibacterial agents widely applied to agriculture, animal husbandry and clinical medicine, and about 50% -90% of the antibiotics can enter soil and water environments along with waste and wastewater in the form of raw materials or metabolites after being used to form sustainable residues. The residues not only can disturb the microbial community structure of an ecological system, but also can induce environmental microorganisms to generate drug-resistant genes, and threaten human health after being diffused through a food chain, so that screening of high-efficiency sulfonamide antibiotic degrading microorganisms becomes one of the important research points in the field of environmental remediation. Microbial degradation is an environment-friendly technology for treating sulfachloropyridazine pollution, and at present, sulfachloropyridazine degrading bacteria of a plurality of genera are separated from activated sludge, soil or water environment and comprise acinetobacter (Acinetobacter sp.), pseudomonas (Pseudomonas sp.) and bacillus (Bacillus sp.) and the like, and the strains can break functional groups of the sulfachloropyridazine through self-metabolizing enzyme systems to realize the conversion or mineralization of pollutants. Campylobacter (ancylobacter sp.) widely distributed in natural environments also exhibits potential in environmental pollution remediation. At present, the research on the campylobacter is focused on the directions of degradation of halogenated organic matters, environmental adaptability and the like. It has been demonstrated by prior studies that strain Ancylobacter aquaticus can grow using 1, 2-dichloroethane as the sole carbon source, achieve efficient degradation of halogenated organics by secretion of dehalogenase, while exhibiting significant environmental stress resistance. However, no research on the degradation of sulfachloropyridazine by this genus of strain has been reported. In view of the application potential of the bacterial strain in the treatment of environmental pollution, the bacterial strain is developed as a novel microbial resource, and has important significance in expanding the degradation path of sulfachloropyridazine. Disclosure of Invention The invention aims to provide sulfachloropyridazine degrading bacteria and application thereof. The sulfachloropyridazine degrading bacterium is campylobacter (Ancylobacters) SAs-18, and the preservation information of the sulfachloropyridazine degrading bacterium is preserved in a preservation unit of Guangdong province microorganism strain preservation center (GDMCC), a preservation date of 2025, 11 and 25 days, a preservation address of Guangzhou city martyr, a 30 th floor of 100 th university in Guangzhou, 5 th floor of Guangdong province microorganism research institute, a mail code of 510070, and a preservation number of GDMCC No.67354. The invention also provides a microbial agent which contains the campylobacter SAs-18 or fermentation liquor thereof as an active ingredient. Preferably, the microbial agent may be a microbial additive. The invention also provides application of the campylobacter SAs-18 or the microbial agent in degrading sulfachloropyridazine. Preferably, the sulfachloropyridazine comprises sulfachloropyridazine in various environments, such as soil and water environments. The invention also provides a method for degrading sulfachloropyridazine, which comprises the steps of inoculating the campylobacter SAs-18 or the microbial agent into an environment containing sulfachloropyridazine, and degrading the sulfachloropyridazine by using the campylobacter SAs-18. The invention separates a strain of campylobacter (Ancylobactrsp.) SAs-18 with good degradation effect on sulfachloropyridazine from the surface soil of a heavily polluted region of a wastewater discharge site of a pharmaceutical factory in Buddha, guangdong, and can be used for degrading and removing sulfachloropyridazine. Provides an economic and green method for removing the novel organic pollutants. Ancylobactrsp. SAs-18, its preservation information is the preservation unit of Guangdong province microorganism strain preservation center (GDMCC), the preservation date is 2025, 11 and 25 days, the preservation address is Guangzhou martyr, 30 th university, 59 th building, 5 th Guangdong province microorganism institute, post code 510070, and the preservation number is GDMCC No.67354. Drawings FIG. 1 shows colony morphology of strain SAs-18 in LB medium. FIG. 2 shows the degradation effect of SAs-18 strain on sulfachloropyridazine in MSM broth. Detailed Description The following examples are further illustrative of the invention and are n