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CN-121622536-B - Xuecha extract, preparation method and application thereof

CN121622536BCN 121622536 BCN121622536 BCN 121622536BCN-121622536-B

Abstract

The application relates to a snow tea extract, a preparation method and application of the snow tea extract, the preparation method comprises the following steps of S1, mixing crushed raw snow tea with water according to a mass ratio of 1:7-15 to obtain a first mixture, S2, culturing bacillus licheniformis on a culture medium to obtain seed liquid of the bacillus licheniformis, S3, adding the seed liquid serving as zymophyte liquid into the first mixture according to an inoculum size of 1% -5%, fermenting to obtain a fermentation mixture, and S4, filtering the fermentation mixture, and concentrating and drying filtrate to obtain the snow tea extract. The obtained snow tea extract has excellent promoting effect on the expression of klotho genes in human fibroblasts, can be applied to products for improving skin health, and fills the blank of the prior art.

Inventors

  • REN QIANQIAN
  • YUAN YONGLEI
  • Yue Kaiyan
  • ZHU XIAOCHONG
  • QU LIPING
  • WANG FEIFEI
  • MA XIAO
  • GUO ZHENYU

Assignees

  • 云南特色植物提取实验室有限公司
  • 云南贝泰妮生物科技集团股份有限公司
  • 美达丝(上海)生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260203

Claims (8)

  1. 1. A preparation method of a snow tea extract is characterized by comprising the following steps: S1, mixing crushed snow tea raw materials with water according to a mass ratio of 1:7-15 to obtain a first mixture; s2, culturing bacillus licheniformis on a culture medium to obtain seed solution of the bacillus licheniformis; S3, using the seed liquid as fermentation bacteria liquid, adding the seed liquid into the first mixture according to the inoculation amount of 1% -5%, and fermenting to obtain a fermentation mixture; S4, filtering the fermentation mixture, and concentrating and drying the filtrate to obtain the product of the snow tea extract; Wherein the bacillus licheniformis is bacillus licheniformis YK-L9 strain which is deposited in the Guangdong province microorganism strain collection at the 8 th month 21 of 2025, and the deposition number is GDMCC No. 66840.
  2. 2. The method of claim 1, wherein in step S1, the first mixture is further subjected to a steam sterilization treatment at 121 ℃.
  3. 3. The preparation method of claim 1, wherein in the step S2, the recovered bacillus licheniformis YK-L9 strain is inoculated into an LB liquid medium, cultured for 18-36 h at 25-40 ℃, and activated for 1-2 generations to obtain seed liquid of the bacillus licheniformis YK-L9 strain.
  4. 4. The preparation method according to claim 1, wherein in the step S3, the fermentation temperature is 25 ℃ to 40 ℃, the fermentation time is 24 to 48 hours, and the rotation speed of the shaking table is 150 rpm to 220 rpm.
  5. 5. The method according to claim 1, wherein in step S4, the fermentation mixture is filtered using a ceramic membrane having a pore size of 0.1 to 10 μm.
  6. 6. A Xuecha extract is characterized in that the Xuecha extract is obtained by fermenting Xuecha with Bacillus licheniformis as zymogen; Wherein the bacillus licheniformis is bacillus licheniformis YK-L9 strain which is deposited in the Guangdong province microorganism strain collection at the 8 th month 21 of 2025, and the deposition number is GDMCC No. 66840.
  7. 7. Use of a thaumatic tea extract according to claim 6 for the preparation of a cosmetic with anti-ageing, anti-inflammatory and anti-oxidant effects.
  8. 8. A skin care product for promoting the expression of klotho gene in human fibroblasts, wherein the skin care product is added with the Xuecha extract of claim 6.

Description

Xuecha extract, preparation method and application thereof Technical Field The application relates to the technical field of microorganisms, in particular to a Xuecha extract, a preparation method and application of the Xuecha extract. Background The skin is the largest organ of the human body and is the first barrier against external environmental damage. Skin aging is a complex and inevitable biological process driven by both endogenous (e.g., genetic, hormonal levels) and exogenous (e.g., ultraviolet radiation, pollution) factors. In recent years, scientific research has gradually revealed that aging is closely related to the expression control of a series of key genes at the molecular level. The Klotho gene is located on human chromosome 13 (13 q 12), is 50kb in length, and consists of 5 exons. By alternative splicing, klotho can produce two proteins, the membrane protein and the circulating protein. Klotho proteins include alpha, beta and gamma. alpha-Klotho is a glucuronidase, also exhibiting antioxidant and anti-inflammatory properties, and has also been found to be involved in a variety of signaling pathways (e.g., wnt, TGF-beta, p53/p21, and IGF-1 pathways) in the aging mechanism. Therefore, the targeting promotion of the expression level of the endogenous klotho gene of the skin fibroblast is regarded as an intervention strategy with great potential, and is expected to radically delay skin aging and promote skin repair and regeneration. At present, direct supplementation of recombinant Klotho protein or the use of Klotho agonists is a potential intervention means, but the methods have the problems of high production cost, poor protein stability, difficult transdermal absorption, possibility of immunogenicity initiation and the like, so that the application of the method in practice is limited, and the method is especially applied to the field of cosmetics. On the other hand, although some small molecule compounds or biological agents have been reported to regulate klotho expression, most of them have the defects of low specificity, low efficiency, unknown safety, and the like. The search for safe and efficient functional extracts from natural products is an important direction for modern anti-aging research and cosmetic development. Extracts of plant, microbial or natural mineral origin are complex in composition, often have the advantage of multi-target, synergistic effects, and often have good skin compatibility and consumer acceptance. However, to date, few studies have been published reporting a specific extract specifically targeted to "promote klotho gene expression in human skin fibroblasts" and its precise preparation. Disclosure of Invention Based on this, there is an urgent need to develop a new technical solution, which can provide a natural extract with a definite preparation method, enriched active ingredients, and capable of effectively and safely promoting klotho gene expression in human skin fibroblasts, and apply the natural extract to products for resisting skin aging and improving skin health, so as to fill the blank of the prior art. In order to achieve the above purpose, the present application provides a preparation method of a snow tea extract, comprising the following steps: S1, mixing crushed snow tea raw materials with water according to a mass ratio of 1:7-15 to obtain a first mixture; s2, culturing bacillus licheniformis on a culture medium to obtain seed solution of the bacillus licheniformis; s3, using the seed liquid as fermentation bacteria liquid, adding the seed liquid into the first mixture according to an inoculation amount of 1% -5%, and fermenting to obtain a fermentation mixture, wherein the inoculation amount can be 1%, 1.5%, 2%, 2.5%, 3%, 4%, 5% and the like; and S4, filtering the fermentation mixture, and concentrating and drying the filtrate to obtain the Xuecha extract. Preferably, the bacillus licheniformis is bacillus licheniformis YK-L9 strain which is deposited with the microorganism strain collection of Guangdong province at the 8 th month 21 of 2025, wherein the deposited address is the institute of microorganisms of Guangdong province, building 5, 30 th university of Games martyr, and the deposited number is GDMCC No. 66840. The 16S rDNA sequence of the Bacillus licheniformis YK-L9 strain is shown in a sequence table SEQ NO. 1. Preferably, in step S1, the first mixture is also subjected to a steam sterilization treatment at 121 ℃. Preferably, in the step S2, the recovered bacillus licheniformis YK-L9 strain is inoculated into an LB liquid culture medium, cultured for 18-36 hours at 25-40 ℃, and activated for 1-2 generations to obtain seed liquid of the bacillus licheniformis YK-L9 strain. Preferably, in the step S3, the fermentation temperature is 25-40 ℃, the fermentation time is 24-48 h, and the rotation speed of the shaking table is 150-220 rpm. Wherein the fermentation temperature can be 25 ℃,26 ℃,27 ℃,28 ℃,29 ℃,30 ℃, 31 ℃, 32 ℃, 33 ℃, 34 ℃, 3