CN-121647249-B - Application of dsRNA of targeted actin coding gene

Abstract

The invention belongs to the technical field of RNA biological control, and particularly relates to application of dsRNA of targeted actin coding genes. The invention provides application of dsRNA of targeted actin coding genes in preventing and controlling crop anthracnose, wherein the nucleotide sequence of the dsRNA is shown as SEQ ID No.2, the dsRNA is transcribed from a target gene segment of anthrax CaACTIN genes, the nucleotide sequence of the target gene segment of anthrax CaACTIN genes is shown as SEQ ID No.1, and the pathogenicity of mango anthrax and banana anthrax can be obviously reduced by loading the dsRNA with nano-material layered double hydroxide, so that the dsRNA has an application prospect of high-efficiency targeted preventing and controlling crop anthracnose.

Inventors

• LIN CHUNHUA
• Wang Kuaikuai
• GU ZHIPENG
• HAN ZHENYU
• YANG YIDAN
• LIU WENBO
• MIAO WEIGUO

Assignees

• 海南大学

Dates

Publication Date

20260508

Application Date

20260205

Claims (7)

1. 1. The application of the dsRNA of the targeted actin coding gene in preventing and controlling crop anthracnose is characterized in that the nucleotide sequence of the dsRNA is shown as SEQ ID No.2, the dsRNA is transcribed from a target gene segment of anthrax CaACTIN gene, the nucleotide sequence of the target gene segment of anthrax CaACTIN gene is shown as SEQ ID No.1, and the pathogenic bacteria of the anthracnose are Siamese anthracnose Colletotrichum siamense, asian anthracnose Colletotrichum asianum and banana anthracnose Colletotrichum musae.
2. 2. The use according to claim 1, wherein the crop comprises mango, banana.
3. 3. A product for controlling crop anthracnose comprising the dsRNA targeting the actin-encoding gene of claim 1.
4. 4. A product according to claim 3, characterized in that the product further comprises a nanomaterial layered double hydroxide.
5. 5. The product of claim 4, wherein the nanomaterial layered double hydroxide is a magnesium/aluminum layered double hydroxide.
6. 6. A method for controlling anthracnose of crops, which is characterized by comprising the step of applying the product of any one of claims 3-5 to crops, wherein the pathogenic bacteria of the anthracnose are siamese anthracnose Colletotrichum siamense, asian anthracnose Colletotrichum asianum and banana anthracnose Colletotrichum musae.
7. 7. The method of claim 6, comprising spraying the product onto mango or banana fruit.

Description

Application of dsRNA of targeted actin coding gene Technical Field The invention belongs to the technical field of RNA biological control, and particularly relates to application of dsRNA of targeted actin coding genes. Background Anthrax (Colletotrichum genus) is one of the most important fungal plant pathogens worldwide, and the anthrax caused by it is serious to crops, cash crops and horticultural plants, often resulting in dead leaves, rotted fruits and sharp reductions in yield. In tropical, subtropical and mediterranean regions, anthracnose causes the quality of export fruits to be reduced, wherein mango and banana are taken as important export fruits in tropical subtropical regions, the anthracnose is more high, the commodity value is seriously reduced, obvious economic loss is often caused after harvesting, and the important threat to the global mango and banana industry is formed. RNA pesticides are novel biopesticides developed based on RNA interference technology, and are essentially specifically combined with mRNA transcribed by specific genes in target organisms, and degradation or inhibition of translation of transcripts is caused through RNAi pathways naturally existing in the target organisms, so that normal growth of the target organisms and harm to host plants are interfered, and finally the purposes of pest control and plant protection are achieved. RNA pesticides are defined as biological pesticides, which have many advantages over traditional chemical pesticides, firstly, dsRNA has stronger specificity and high-efficiency gene silencing capability and does not involve transgenic technology, secondly, according to the design of targets, dsRNA can inhibit the growth of viruses, bacteria and fungi so as to have control effects on diseases caused by the viruses, bacteria and fungi, in addition, dsRNA can penetrate cell membranes and be transmitted in vivo, and finally, dsRNA is easy to degrade in the environment, so that the pesticide is an environment-friendly pesticide. Based on the method, the RNA pesticide has the advantages of obvious disease and pest control advantages of high specificity, good effect, difficult resistance generation, no toxicity, no harm and no residue, and low research and development cost, and is the front direction of green prevention and control of agriculture. However, the application of RNA pesticides is also accompanied by a number of challenges, such as the fact that naked dsRNA is easily degraded by enzymes and ultraviolet rays in the environment, resulting in short efficacy, the pest epidermis blocks the absorption of dsRNA, resulting in low dsRNA delivery efficiency, affecting the silencing effect of target genes, and high cost for large-scale synthesis of dsRNA. Therefore, how to obtain the high-efficiency control and targeting optimized anthracnose RNA pesticide is a problem to be solved in the field. Disclosure of Invention The invention aims to provide an application of dsRNA of targeted actin coding genes, which can efficiently and targetedly prevent and treat crop anthracnose. In order to achieve the above purpose, the invention adopts the following technical scheme: The invention provides application of dsRNA of targeted actin coding genes in preventing and controlling crop anthracnose, wherein the nucleotide sequence of the dsRNA is shown as SEQ ID No.2, the dsRNA is transcribed from a target gene segment of anthrax CaACTIN, and the nucleotide sequence of the target gene segment of anthrax CaACTIN is shown as SEQ ID No. 1. Preferably, the crops comprise mangoes and bananas. Preferably, the pathogenic bacteria of the anthracnose include Siamese anthracnose Colletotrichum siamense, asian anthracnose Colletotrichum asianum and banana anthracnose Colletotrichum musae. The invention also provides a product for preventing and treating crop anthracnose, which comprises the dsRNA of the targeted actin coding gene. Preferably, the product further comprises a nanomaterial layered double hydroxide (Layered double hydroxides, LDH). More preferably, the nanomaterial layered double hydroxide is a magnesium/aluminum layered double hydroxide. The invention also provides a method for controlling anthracnose of crops, which comprises the step of applying the product to the crops. More preferably, it comprises spraying the above-mentioned product onto mango or banana fruit. The invention has the beneficial effects that: Firstly, segment dsRNA of a target gene is obtained through an in-vitro dsRNA synthesis technology, then the segment dsRNA is compounded with nano material Layered Double Hydroxide (LDH) to obtain a dsRNA nano composition for controlling crops, the dsRNA nano composition is sprayed on mango fruits and banana fruits, siamese anthrax Colletotrichum siamense-2, asian anthrax Colletotrichum asianum-3 and banana anthrax Colletotrichum musae-1 are respectively inoculated on the mango fruits and the banana fruits, the area of a disease spot is measured, and