CN-121653020-B - Marine bacillus belay SCSX, biocontrol compound microbial inoculum, preparation method and application thereof

Abstract

The invention relates to the technical field of microbial technology and biological control of postharvest diseases of fruits and vegetables, in particular to marine bacillus beijerinckii SCSX, a biological control compound microbial inoculum, and a preparation method and application thereof. The marine bacillus belicus SCSX is obtained through separation and screening, and the strain and fermentation products (bacterial suspension and bacterial fermentation liquid) and the green biocontrol microbial inoculum thereof can effectively inhibit various pathogenic bacteria, thereby safely and effectively controlling the occurrence and development of plant diseases. The biological control compound microbial inoculum further comprising bacillus bailii 12Y can generate various antibacterial substances, and the antibacterial broad spectrum, stability and prevention and control efficiency are obviously improved through the synergistic effect among strains, meanwhile, the fruit fresh-keeping period and storage time are prolonged, the quality of strawberries and mangoes is improved, and the biological control compound microbial inoculum has higher economic significance in agricultural production.

Inventors

• XIAO HONGMEI
• REN YUBIN
• WANG XIUJING
• ZHANG QIUQIN
• Cui Minyi
• WEI YANSHU
• GUO XINQI
• Zhao Meiqian
• WANG HUIHENG

Assignees

• 南京农业大学三亚研究院

Dates

Publication Date

20260508

Application Date

20260206

Claims (4)

1. 1. The biocontrol compound microbial inoculum is characterized in that a bacterial fermentation liquid of marine source bacillus bailii (Bacillus velezensis) and a bacterial fermentation liquid of bacillus bailii (Bacillus velezensis) 12Y are uniformly mixed according to a volume ratio of 1:1, and the biocontrol compound microbial inoculum is obtained; Wherein, the marine source bacillus belay is classified and named as bacillus belay Bacillus velezensis, the strain number is SCSX, and the marine source bacillus belay is preserved in China general microbiological culture collection center (CGMCC) No.35916 in the 9 th month 15 th year 2025, and the preservation address is the No.3 of the West way 1 of the Chaoyang area North Star of Beijing city; Wherein the preservation number of the bacillus bailii 12Y is CGMCC No.28636.
2. 2. The method for preparing the biocontrol compound microbial inoculum of claim 1, which is characterized in that the biocontrol compound microbial inoculum is obtained by uniformly mixing a bacterial fermentation liquid of marine source bacillus beliensis and a bacterial fermentation liquid of bacillus beliensis 12Y according to a volume ratio of 1:1.
3. 3. The method according to claim 2, wherein the total concentration of the composite microbial inoculum is 1 x 10 7 CFU/mL~1×10 9 CFU/mL.
4. 4. The use of the biocontrol compound microbial inoculum of claim 1 for preserving or controlling fungal diseases after fruit harvest; Wherein the fruit is strawberry and/or mango, and the fungal disease is any one or a combination of a plurality of strawberry gray mold, strawberry black spot, strawberry anthracnose and mango anthracnose.

Description

Marine bacillus belay SCSX, biocontrol compound microbial inoculum, preparation method and application thereof Technical Field The invention relates to the technical field of microbial technology and biological control of postharvest diseases of fruits and vegetables, in particular to marine bacillus beijerinckii SCSX, a biological control compound microbial inoculum, and a preparation method and application thereof. Background Fruit postharvest fungal disease is an important factor affecting fruit storage quality and commodity value, such as strawberry gray mold (caused by Botrytis cinerea), strawberry black spot (caused by ALTERNARIA ALTERNATA, etc.), strawberry anthracnose and mango anthracnose (mainly caused by Colletotrichum genus fungi) are common postharvest diseases. Typical symptoms of these diseases are seen as brown or black lesions on the surface of the fruit and spread gradually leading to decay of the fruit. The strawberry peel is soft and fragile, the mango is easy to suffer mechanical damage after being picked, the pulp of the strawberry peel and the mango pulp are soft and juicy and rich in bioactive substances, and the strawberry peel is more easy to suffer pathogen infection after being picked, so that the incidence rate of strawberry fungal diseases reaches 25-30%, and the loss rate of the mango after being picked reaches 20-30%, which seriously affects the storage quality and commodity value of fruits. The main control means for the diseases at present comprise physical methods such as low-temperature storage, heat treatment and radiation, which can inhibit the growth of pathogenic bacteria but damage the quality of fruits easily, chemical methods such as bactericides such as benzimidazoles, carbendazim and the like, which are effective but easy to generate harmful substance residues, promote the pathogenic bacteria to generate drug resistance and also cause environmental pollution, and biological methods such as preventing and controlling the diseases by using biocontrol bacteria such as bacillus, saccharomycetes, pseudomonas and the like through nutrition competition, antibiotic generation and other modes, and have the advantages of safety and sustainability. However, the current commonly used biocontrol bacteria are mostly derived from soil or plant endophytic environment, and have obvious defects that (1) the bacteriostasis spectrum is narrow, complex infection caused by different fungi such as Botrytis cinerea (Botrytis cinerea), alternaria (ALTERNARIA ALTERNATA) and Colletotrichum (Colletotrichum) fungi is difficult to cope with, the control effect is unstable, the influence of different environmental factors is easy to cause, and (3) the fruits are often subjected to cross infection of multiple pathogenic bacteria after being picked, and the comprehensive capability of coping with complex disease systems is lacking. Therefore, although biological control is regarded as a potential green prevention and control way, the existing biological control microbial inoculum still has significant defects in bacteriostasis broad spectrum, environmental stability and practical application effect, and development of a novel biological control preparation with wider bacteriostasis spectrum, stronger environmental adaptability and synergistic prevention effect is needed to cope with the prevention and control requirements of complex fungal diseases after fruit harvest. Disclosure of Invention The invention provides marine bacillus bailii, which aims to solve the technical problems of narrow biocontrol bacteria inhibition spectrum, poor stability and poor practical application effect in the prior art. The technical problem to be solved by the second aspect of the invention is to provide a microbial inoculum. The technical problem to be solved by the third aspect of the invention is to provide application of the marine bacillus behenii in pathogen inhibition. The technical problem to be solved by the fourth aspect of the invention is to provide the application of the marine bacillus behenii in fruit postharvest preservation and fungal disease prevention. The technical problem to be solved by the fifth aspect of the invention is to provide a biocontrol compound microbial inoculant. The technical problem to be solved by the sixth aspect of the invention is to provide a preparation method of the biocontrol compound microbial inoculum. The seventh aspect of the invention aims to provide the application of the biocontrol compound microbial inoculum in fruit postharvest preservation and fungal disease prevention. In order to solve the technical problems, the invention adopts the following technical scheme: In a first aspect, the invention provides a marine bacillus belay, which is classified and named as bacillus belay Bacillus velezensis, has a strain number of SCSX, is preserved in China general microbiological culture collection center (CGMCC) for 9 and 15 days of 2025, has a preservation number of C