CN-121653219-B - Extraction method of high-activity ginseng extract

Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to an extraction method of a high-activity ginseng extract, which is used for improving the conversion and extraction efficiency of rare ginsenoside Rg1, rg3, rg5, rh2 and Compound K components, the extraction method is combined with microbial fermentation, electrostatic field wall breaking and supercritical CO 2 extraction, wherein microbial fermentation treatment is mainly used for promoting the synthesis of ginsenoside, a high-voltage pulse electric field is used for inducing ginseng cells to generate electroporation effect, so that the permeability of cell membranes and cell walls is increased, the supercritical CO 2 extraction has higher cell permeability, the ginsenoside extraction rate and purity are improved, and in addition, a catalytic aid added in the extraction process is beneficial to the conversion catalysis of the rare ginsenoside components.

Inventors

• ZHENG FEI
• SUN FENG
• YUE HAO
• PI ZIFENG

Assignees

• 长春中医药大学

Dates

Publication Date

20260508

Application Date

20260206

Claims (4)

1. 1. The extraction method of the high-activity ginseng extract is characterized by comprising the following steps of: step 1, cleaning, drying and crushing ginseng to obtain ginseng powder, and fermenting the ginseng powder to obtain fermentation powder; step 2, treating the fermentation powder by utilizing a high-voltage pulse electric field to obtain pretreated fermentation powder; Step 3, mixing the pretreated fermentation powder, a catalytic auxiliary agent and absolute ethyl alcohol, extracting by supercritical CO 2 , and collecting an extract; Step 4, performing ultrafiltration centrifugation, purification by adsorption resin, eluting and collecting the eluent; step 5, decoloring, concentrating and drying the eluent to obtain a high-activity ginseng extract; In the step1, the fermentation treatment process uses a beta-glucosidase high-yield strain; the beta-glucosidase high-yield strain is selected from any one of lactobacillus plantarum LP115, lactobacillus plantarum subspecies ATCC14917 and lactobacillus mucilaginosus CECT 5716; In the step 3, the catalyst auxiliary agent comprises the following raw materials by mass percent, 1% of polysorbate 80, 1.5% of beta cyclodextrin, 0.4% of citric acid, 0.3% of sodium citrate, 0.4% of calcium chloride, 1% of beta-glucosidase, 1% of beta-xylosidase, 4.4% of microcrystalline cellulose and 90% of purified water; the mass ratio of the pretreated fermentation powder to the catalytic auxiliary agent to the absolute ethyl alcohol is 10:1.5:1.
2. 2. The method according to claim 1, wherein in step 2, the parameters of the high-voltage pulsed electric field are set to a field strength of 20-25 kV/cm, a pulse frequency of 1000 Hz, a time of 10-15 min, and a temperature of 40 ℃.
3. 3. The method according to claim 1, wherein in step 3, the supercritical CO 2 extraction process is performed at a pressure of 25-35 MPa, a CO 2 flow rate of 150L/h, an extraction temperature of 45-55deg.C, and an extraction time of 1-2 h.
4. 4. The method according to claim 3, wherein in step 4, the ultrafiltration centrifugation process uses an ultrafiltration tube having a molecular weight of 10 kDa; In step 5, the decolorizing treatment process uses a decolorizing resin column.

Description

Extraction method of high-activity ginseng extract Technical Field The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to an extraction method of a high-activity ginseng extract. Background The research of the extraction process of ginsenoside is an important field of traditional Chinese medicine active ingredient development and modern preparation technology. The traditional extraction method such as decoction, impregnation, reflux extraction and the like has the characteristics of simple equipment and convenient operation, but the extraction efficiency is generally low, a large amount of solvent and time are often consumed, and the hydrolysis or isomerization of the thermosensitive saponin component is easily caused in the continuous heating process, so that the activity of a target product is reduced. In recent years, modern extraction techniques, represented by ultrasonic extraction, microwave-assisted extraction, supercritical CO 2 fluid extraction, and the like, have shown significant progress in improving the extraction rate and reducing the solvent usage. For example, ultrasonic extraction promotes cell disruption by cavitation, microwaves accelerate component dissolution by an internal heating mechanism, and supercritical CO 2 helps maintain component stability by virtue of its high permeability and low temperature operation. However, the technology still faces common challenges in practical application, on one hand, the extraction process is mainly based on a physical principle, the selectivity of saponin components is lacked, and rare ginsenoside is difficult to directionally enrich, and on the other hand, the rare saponin is extremely low in content in raw materials and is mostly tightly combined with a cell structure, so that the efficient release and conversion of the rare saponin are difficult to realize only by a physical means, the proportion of the rare saponin in the final extract is difficult to be obviously improved, and the development of related high-added-value products is restricted. Disclosure of Invention Aiming at the situation, the invention provides an extraction method of a high-activity ginseng extract, which aims to construct a green and efficient ginseng extraction process so as to improve the content and the extraction rate of high-activity rare ginsenoside in the ginseng extract, and combines microbial fermentation, electrostatic field wall breaking and supercritical CO 2 extraction to realize efficient dissolution and stable activity of the ginsenoside under mild conditions. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: The invention provides an extraction method of a high-activity ginseng extract, which specifically comprises the following steps: step 1, cleaning, drying and crushing ginseng to obtain ginseng powder, taking a beta-glucosidase high-yield strain as a fermentation strain, culturing and amplifying to obtain seed liquid, wetting the ginseng powder with purified water, sterilizing by high-pressure steam to avoid mixed bacteria pollution to obtain sterilized materials, spraying the seed liquid on the surface of the sterilized materials, placing the sterilized materials in a fermentation tank for fermentation for 5 days, promoting the generation of rare saponin components Rg1, rg3, rh2 and Rg5 in the ginseng, and drying at 90 ℃ to perform microorganism and enzyme inactivation to obtain the fermentation powder; Step 2, uniformly spreading the fermentation powder in a parallel plate electrode device, and adopting a high-voltage pulse electric field to treat the fermentation powder, wherein the field strength is 20-25 kV/cm, the pulse frequency is 1000 Hz, the time is 10-15 and min, and the temperature is 40 ℃, and the high-voltage pulse electric field is used for inducing ginseng cells to generate electroporation effect, so that the permeability of cell membranes and cell walls is increased, the diffusion of cell contents to extracellular media is promoted, and polar saponin components are more easily dissolved out, so that the pretreated fermentation powder is obtained; Extracting and extracting pretreated fermentation powder by using a catalytic auxiliary agent and supercritical CO 2, mixing the pretreated fermentation powder, the catalytic auxiliary agent and absolute ethyl alcohol according to a mass ratio of 10:1.5:1, adding the mixture into a high-pressure extraction kettle of a supercritical extraction device, wherein a solubilizing agent and an inclusion effect provided by the catalytic auxiliary agent are beneficial to promoting dissolution of saponin components, the added glycosidase can promote directional catalytic conversion of primary saponin substrates Rb1, rc and Rd into rare ginsenoside Rg3 and Compound K, the CO 2 is used as an extracting agent, the extraction pressure is set to be 25-35 MPa, the flow rate of CO 2 is 150L/h, the ex