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CN-121667041-B - Method for planting white ginseng fungus by using pinus massoniana stump

CN121667041BCN 121667041 BCN121667041 BCN 121667041BCN-121667041-B

Abstract

The invention provides a method for planting white ginseng fungus by using pinus massoniana stems, and relates to the technical field of artificial planting of white ginseng fungus. The method for planting the white ginseng fungus by the pinus massoniana stake comprises the steps of sterilizing old stake/new stake after pinus massoniana is harvested by adopting a blast lamp, spraying aqueous solution of the buddleia brine extract, airing, inoculating liquid spawn of the white ginseng fungus, and culturing the liquid spawn of the white ginseng fungus by adopting almond pulp, the buddleia brine extract and a potato liquid culture medium as culture mediums. The invention overcomes the defects of the prior art, and effectively promotes the growth of the white ginseng fungus on the stumps, increases the yield and improves the economic benefit by sterilizing the stumps and using the nutritional agent and adjusting the culture medium components of the white ginseng fungus.

Inventors

  • CHEN WEI
  • LI JIANG
  • JING YUEBO
  • LUO TING
  • Hu Tanggeran

Assignees

  • 云南省林业和草原科学院

Dates

Publication Date
20260512
Application Date
20260205

Claims (9)

  1. 1. A method for planting white ginseng fungus by using pinus massoniana stems, which is characterized by comprising the following steps: s1, adopting new piles or old piles which are harvested by pinus massoniana as planting materials, and adopting a blast lamp to burn and sterilize the surfaces of the new piles or the old piles 2 months before the beginning of a rainy season to obtain sterilized piles for later use; S2, selecting white ginseng fruiting bodies naturally growing on the pinus massoniana in the pinus massoniana forest as strains, inoculating the strains into a potato protein culture medium, and culturing mycelia; S3, mixing mycelium with sterile water, grinding to prepare a suspension, and inoculating the suspension into a liquid culture medium with a formula of 10-20mL of almond pulp, 600-800mg of buddleia brine extract and 1L of potato liquid culture medium for culture to obtain liquid strains; S4, preparing 200-400mg/L of nutrient of the buddleia officinalis brine extract, pouring the sterilized nutrient on the surface of the sterilized pile material for once on a sunny day, and naturally airing and drying to obtain a pretreated pile material for later use; s5, pouring the liquid strain onto the pretreated piles, completing inoculation, and harvesting after 3-4 months.
  2. 2. The method of claim 1, wherein the spraying lamps in the step S1 burn and sterilize the new pile or the old pile, and then burn and sterilize vegetation around the new pile or the old pile, wherein the treatment range is 20-50cm of the radius of the new pile or the old pile.
  3. 3. The method of claim 1, wherein the potato protein medium comprises 200g of potato, 20g of glucose, 3g of peptone, 2g of monopotassium phosphate, 18g of agar, 0.5g of magnesium sulfate and 1L of water.
  4. 4. The method of claim 1, wherein the potato liquid medium comprises 200g of potato, 20g of glucose, 3g of peptone, 2g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate and 1L of water.
  5. 5. The method according to claim 1, wherein the culturing conditions in the step S2 are culturing for 8d at 24 ℃ plus or minus 1 ℃ in a dark place, and the culturing conditions in the step S3 are culturing for 5d at 24 ℃ plus or minus 1 ℃ in a dark place on a shaking table at 120 r/min.
  6. 6. The method of claim 1, wherein the almond paste is prepared by grinding the parched almonds with 5-8 times of clear water to paste, boiling for 20-30min, filtering, and removing residues.
  7. 7. The method according to claim 1, wherein the method of preparing the brine extract of buddleia comprises the steps of: (1) Cleaning and grinding the whole fresh buddleia officinalis into slurry to obtain the whole slurry for standby; (2) Adding clear water with the volume of 3-5 times of that of the whole plant slurry, adding sodium chloride, adjusting the content of the sodium chloride to 10% -12%, performing ultrasonic vibration for 10-20min, and squeezing and filtering to obtain filtrate for later use; (3) Spray drying the filtrate to obtain the salt water extract of buddleia.
  8. 8. The method of claim 7, wherein the power of the ultrasonic vibration in the step (2) is 400-600W.
  9. 9. The method of claim 7, wherein the inlet air temperature of spray drying in the step (3) is 180 ℃, the outlet air temperature is 90 ℃, and the treatment capacity is 80kg/h.

Description

Method for planting white ginseng fungus by using pinus massoniana stump Technical Field The invention relates to the technical field of artificial planting of white ginseng fungus, in particular to a method for planting white ginseng fungus by using pinus massoniana stems. Background The pinus koraiensis is an evergreen arbor of pinus genus of pinaceae family, and the trunk end is straight and not twisted, and the pinus koraiensis is generally used as wood with a short growth period, so that more new and old stumps after felling are usually present in a pinus koraiensis forest farm. The schizophyllum commune, the white flower, the sarcandra, and the like are precious fungi used as both food and medicine, and belong to the kingdom of fungi, basidiomycota and schizophyllaceae. The fungus cover is fan-shaped or kidney-shaped, has a diameter of 1-5 cm, has a white to off-white surface, has a plurality of split leaves, has thin and tough fungus meat, has fresh and delicious taste and rich nutrition, and the white ginseng fungus is wood-rotting fungus, and is generally found on broadleaf trees (such as oak, castanopsis quassia and tannin) and dead branches of conifer trees, so that the yield of the wild white ginseng fungus is limited and cannot meet the market demand, the artificial cultivation white ginseng fungus is a great development direction in the current stage, the artificial cultivation is mostly carried out by taking agricultural and sideline products rich in cellulose such as cotton seed hulls, corncobs, bagasse, wood chips and the like as main materials, adding auxiliary materials such as wheat bran, rice bran and the like and trace mineral elements as culture mediums of the white ginseng fungus, and taking the auxiliary materials and trace mineral elements as nursing culture mediums in indoor greenhouse, and for the curcas in the Yunnan area, more new and old piles can be used as natural culture matrixes of the white ginseng fungus, but the whole yield is low, and the economic value of direct inoculation white ginseng fungus is not high. Therefore, how to culture white ginseng fungus by using the residue after slashing the pinus massoniana and improving the economic benefit of planting are important research directions at the present stage. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a method for planting white ginseng fungus by using pinus massoniana stump, which effectively promotes the growth of the white ginseng fungus on the stump, increases the yield and improves the economic benefit by sterilizing the stump and using a nutrient, and adjusting the culture medium components of the white ginseng fungus. In order to achieve the above purpose, the invention is realized by the following technical scheme: a method for planting white ginseng fungus by using pinus massoniana stems, comprising the following steps: s1, adopting new piles or old piles which are harvested by pinus massoniana as planting materials, and adopting a blast lamp to burn and sterilize the surfaces of the new piles or the old piles 2 months before the beginning of a rainy season to obtain sterilized piles for later use; S2, selecting white ginseng fruiting bodies naturally growing on the pinus massoniana in the pinus massoniana forest as strains, inoculating the strains into a potato protein culture medium, and culturing mycelia; S3, mixing mycelium with sterile water, grinding to prepare a suspension, and inoculating the suspension into a liquid culture medium with a formula of 10-20mL of almond pulp, 600-800mg of buddleia brine extract and 1L of potato liquid culture medium for culture to obtain liquid strains; S4, preparing 200-400mg/L of nutrient of the buddleia officinalis brine extract, pouring the sterilized nutrient on the surface of the sterilized pile material for once on a sunny day, and naturally airing and drying to obtain a pretreated pile material for later use; s5, pouring the liquid strain onto the pretreated piles, completing inoculation, and harvesting after 3-4 months. Preferably, in the step S1, the torch burns and sterilizes the surface of the new pile or the old pile, and then burns and processes vegetation around the new pile or the old pile, and the processing range is 20 cm to 50cm of the radius of the new pile or the old pile. Preferably, the potato protein culture medium comprises 200g of potato, 20g of glucose, 3g of peptone, 2g of monopotassium phosphate, 18g of agar, 0.5g of magnesium sulfate and 1L of water. Preferably, the potato liquid culture medium comprises 200g of potato, 20g of glucose, 3g of peptone, 2g of monopotassium phosphate, 0.5g of magnesium sulfate and 1L of water. Preferably, the culture condition in the step S2 is that the culture is carried out for 8 days at the temperature of 24 ℃ plus or minus 1 ℃ in a dark way, and the culture condition in the step S3 is that the culture is carried out for 5 days at the temperature of 120r/min and 24