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CN-121667247-B - Stress-resistant yield-increasing plant growth regulator, and preparation method and application thereof

CN121667247BCN 121667247 BCN121667247 BCN 121667247BCN-121667247-B

Abstract

The invention discloses a stress-resistant yield-increasing plant growth regulator, and a preparation method and application thereof, belonging to the field of plant growth regulators. The stress-resistant yield-increasing plant growth regulator comprises the following raw materials of a suaeda salsa extract, a stress-resistant biostimulant, propionyl brassinolide, coronatine, polyaspartic acid, gamma-aminobutyric acid, an emulsifying agent, a penetrating agent, an auxiliary emulsifying agent, a pH buffering agent and deionized water, wherein the stress-resistant biostimulant is sclareolide. The invention obviously improves the tolerance of wheat to the saline-alkali stress by strengthening the antioxidant system and the osmotic adjustment capability, ensures the basic survival and normal physiological functions of the wheat, and effectively reverses the growth inhibition caused by stress by promoting the root system development, enhancing photosynthesis, driving the product to transfer to seeds and other mechanisms, thereby realizing the obvious yield improvement under adverse circumstances.

Inventors

  • ZHOU YIXIN
  • Qin Xiangqi
  • LIU SHENGWU
  • WU HAN
  • HU LINJUN
  • Jiao Yineng
  • ZHOU CAN
  • LIU XIAODAN
  • HUANG JIE
  • CHEN WANYANG
  • FAN DENG
  • YAN LAI
  • ZHANG XIAOYANG

Assignees

  • 湖北茂盛生物有限公司

Dates

Publication Date
20260505
Application Date
20260209

Claims (5)

  1. 1. The stress-resistant yield-increasing plant growth regulator is characterized by comprising, by mass, 0.04-0.06% of suaeda salsa extract, 0.01-0.02% of stress-resistant biostimulant, 0.001-0.002% of propionyl brassinolide, 0.01-0.05% of coronatine, 0.5-2% of polyaspartic acid, 0.8-1% of gamma-aminobutyric acid, 3-8% of emulsifier, 2-5% of penetrating agent, 1-2% of auxiliary emulsifier, 0.1-0.3% of pH buffering agent and 100% of deionized water; the stress-resistant biostimulant is sclareolide, the emulsifier is fatty alcohol polyoxyethylene ether, the penetrating agent is sodium lignin sulfonate, and the auxiliary emulsifier is propylene glycol.
  2. 2. The stress-tolerant yield-increasing plant growth regulator according to claim 1, wherein the preparation method of the suaeda salsa extract comprises the steps of: (1) Removing impurities from fresh Suaeda salsa stems and leaves, cleaning with water, oven drying at 40-50deg.C until the water content is less than 8%, pulverizing, and sieving with 40-60 mesh sieve to obtain Suaeda salsa powder; (2) Mixing Suaeda salsa powder and 0.5-1mol/L citric acid aqueous solution according to the mass volume ratio of 1g to 15mL, uniformly stirring, heating to 80-90 ℃, leaching at constant temperature for 2 times, each time for 1.5-2h, adding anhydrous sodium sulfate accounting for 1-2% of the total mass of the Suaeda salsa powder and the citric acid aqueous solution in the leaching process, filtering after the first leaching, adding an equal amount of water into filter residues for secondary leaching, and obtaining a Suaeda salsa aqueous extract from the filtrate obtained from the two leaching; (3) Pouring the suaeda salsa water extract into a rotary evaporator, concentrating to 1/5 of the original volume to obtain a concentrated solution, adding absolute ethyl alcohol into the concentrated solution to enable the volume fraction of the ethyl alcohol in the system to reach 60%, standing for 12-24h at 4 ℃, filtering by a filter, and collecting filtrate; (4) Concentrating the filtrate with rotary evaporator again to obtain thick paste concentrate, and vacuum freeze drying or spray drying to obtain Suaeda salsa extract.
  3. 3. A method for preparing the stress-resistant yield-increasing plant growth regulator according to any one of claims 1 to 2, comprising the steps of: Heating deionized water to 40-50 ℃, adding polyaspartic acid and gamma-aminobutyric acid, stirring until the polyaspartic acid and gamma-aminobutyric acid are completely dissolved, adding an emulsifying agent and an auxiliary emulsifying agent, stirring uniformly to form a water phase, dissolving propionyl brassinolide, coronatine and an anti-stress biological stimulant in ethanol, slowly adding the propionyl brassinolide, coronatine and an anti-stress biological stimulant into the water phase, shearing for 20 minutes at 3000-5000r/min, finally adding a suaeda salsa extract and a penetrating agent, stirring uniformly, cooling to room temperature, and adding a pH buffering agent to obtain the final product of the plant growth regulator.
  4. 4. Use of a stress-tolerant plant growth regulator according to claim 1, in particular for increasing the stress tolerance of crops in saline-alkali soil and for increasing the crop yield.
  5. 5. The use according to claim 4, wherein the crop is wheat.

Description

Stress-resistant yield-increasing plant growth regulator, and preparation method and application thereof Technical Field The invention belongs to the technical field of plant growth regulators, and particularly relates to a stress-resistant yield-increasing plant growth regulator, and a preparation method and application thereof. Background With the increasing shortage of cultivated land resources worldwide, soil salinization has become an important environmental factor restricting agricultural production. The saline-alkali stress causes the increase of the osmotic pressure of soil and the aggravation of ion poisoning, seriously inhibits the root system development and the water and nutrient absorption of crops, and causes the accumulation of active oxygen and the peroxidation of membrane lipid, thereby finally causing the growth resistance of plants, the reduction of photosynthetic efficiency and the remarkable reduction of yield. Traditional saline-alkali soil improvement modes such as hydraulic engineering, chemical improvement and the like have high cost and long period, and are difficult to quickly take effect in crop growing seasons. At present, although single or simple compound plant growth regulators can relieve adverse stress to a certain extent, most products have limited functions, often focus on a certain physiological link (such as root promotion or antioxidation), and are difficult to cooperatively realize the dual goals of stress resistance and keep-alive and stable yield and income increase in saline-alkali soil practice. Especially for main grain crops such as wheat, the yield formation of the main grain crops is easy to be inhibited in a saline-alkali environment, and a compound regulator capable of systematically regulating multiple stress-resistant paths and simultaneously enhancing photosynthesis and yield characteristics is urgently needed. In recent years, the use of components such as natural plant extracts has been attracting attention. While Suaeda salsa is a typical halophyte, is rich in permeation regulating substances and antioxidant components, but has limited single use effect, signal substances such as brassinolide, coronatine and the like have core effects in regulating growth and stress resistance response, and how to realize functional complementation and synergistic interaction among components through scientific compatibility becomes a key for developing a high-efficiency stress resistance and yield increase product. Under the background, the invention constructs a multi-channel synergistic composite regulator by integrating various substances such as suaeda salsa active extract, propionyl brassinolide, coronatine, sclareolide and the like, and aims to activate antioxidant defense, osmotic regulation, photosynthesis promotion and system stress resistance signal network in crops at the same time, thereby guaranteeing normal physiological functions of wheat under saline-alkali stress, remarkably improving yield potential of the wheat and providing an effective solution for sustainable development of agriculture in saline-alkali lands. Disclosure of Invention The invention aims to provide a stress-resistant yield-increasing plant growth regulator which improves the stress resistance of wheat in saline-alkali soil, promotes the growth of wheat and increases the yield. In order to achieve the technical purpose, the invention adopts the following technical scheme: a stress-resistant yield-increasing plant growth regulator comprises, by mass, 0.04-0.06% of suaeda salsa extract, 0.01-0.02% of stress-resistant biostimulant, 0.001-0.002% of propionyl brassinolide, 0.01-0.05% of coronatine, 0.5-2% of polyaspartic acid, 0.8-1% of gamma-aminobutyric acid, 3-8% of emulsifier, 2-5% of penetrating agent, 1-2% of auxiliary emulsifier, 0.1-0.3% of pH buffering agent and 100% of deionized water. Further, the preparation method of the suaeda salsa extract comprises the following steps: (1) Removing impurities from fresh Suaeda salsa stems and leaves, cleaning with water, oven drying at 40-50deg.C until the water content is less than 8%, pulverizing, and sieving with 40-60 mesh sieve to obtain Suaeda salsa powder; (2) Mixing Suaeda salsa powder and 0.5-1mol/L citric acid aqueous solution according to the mass volume ratio of 1g to 15mL, uniformly stirring, heating to 80-90 ℃, leaching at constant temperature for 2 times, each time for 1.5-2h, adding anhydrous sodium sulfate accounting for 1-2% of the total mass of the Suaeda salsa powder and the citric acid aqueous solution in the leaching process, filtering after the first leaching, adding an equal amount of water into filter residues for secondary leaching, and obtaining a Suaeda salsa aqueous extract from the filtrate obtained from the two leaching; (3) Pouring the suaeda salsa water extract into a rotary evaporator, concentrating to 1/5 of the original volume to obtain a concentrated solution, adding absolute ethyl alcohol into the conc