CN-121668311-B - Antibacterial photodynamic force for treating periodontitis by micro-environment response regulation and control of ROS (reactive oxygen species) and preparation method thereof

Abstract

The invention belongs to the technical field of antibacterial photodynamic, and provides an antibacterial photodynamic for treating periodontitis by regulating and controlling ROS in a microenvironment response mode and a preparation method thereof. Comprises preparing tin oxide by using sodium dodecyl benzene sulfonate, stannic chloride pentahydrate and L-cysteine as raw materials, preparing nano enzyme by using zinc nitrate, copper nitrate, urea and thioacetamide as raw materials, and preparing antibacterial photodynamic by using indocyanine green, bacterial targeting peptide, nano enzyme, tin oxide and dihydromyricetin as raw materials. The invention constructs micro-environment response antibacterial photodynamic aiming at periodontitis infection and treatment characteristics, and proposes a strategy combining the programmed regulation and control of ROS (reactive oxygen species), accurate, long-acting treatment and immune regulation of bacterial infection, and constructs antibacterial photodynamic, so that the curative effect and the scene of the antibacterial photodynamic for treating periodontitis are improved and widened.

Inventors

• SONG LI
• XU FANCHENG
• XU TING
• DAI FANG
• WU JIANXIN
• DENG TIAN
• SONG CHAORU
• HUANG TIANYU
• Tian Mawen

Assignees

• 南昌大学第二附属医院
• 南昌大学第一附属医院

Dates

Publication Date

20260505

Application Date

20260211

Claims (9)

1. 1. The preparation method of the antibacterial photodynamic system for treating periodontitis by micro-environment response regulation and control of ROS is characterized by comprising the following steps: Mixing sodium dodecyl benzene sulfonate, stannic chloride pentahydrate and L-cysteine into a mixed solution of ethylene glycol and water, stirring at room temperature in a dark place, performing hydrothermal reaction, cooling to room temperature after the reaction is finished, centrifugally collecting and washing to obtain a precipitate, and freeze-drying to obtain tin oxide sulfide; step two, dissolving zinc nitrate, copper nitrate and urea in deionized water, heating and stirring, then heating and maintaining the temperature, after the reaction is finished, centrifugally washing and drying a reaction product, and heating to 350-450 ℃ to obtain a precursor; Dissolving indocyanine green and bacterial targeting peptide UBI 29-41 in ultrapure water according to the mass ratio of 10:1, stirring in a dark place, centrifuging and freeze-drying to obtain a combination product of photosensitizer indocyanine green and bacterial targeting peptide UBI 29-41 , adding nano enzyme and tin sulfide into an ultrapure water solution according to the mass ratio of 2:1, stirring overnight, centrifuging and freeze-drying to obtain nano enzyme-loaded tin sulfide oxide, stirring nano enzyme-loaded tin sulfide, dihydromyricetin and combination product in a dark place according to the mass ratio of 2:1:1 for overnight, centrifuging and washing the reaction product after the reaction is finished, and freeze-drying to obtain the antibacterial photodynamic system.
2. 2. The method according to claim 1, wherein in the first step, 2.4mmol of sodium dodecylbenzenesulfonate, 1.0mmol of stannic chloride pentahydrate and 8.0mmol of L-cysteine are mixed in a mixed solution of ethylene glycol and water.
3. 3. The method of claim 1, wherein the hydrothermal reaction is carried out at a temperature of 150 to 170℃for a period of 8 to 12 hours.
4. 4. The method of claim 1, wherein in the second step, 10.1mmol of zinc nitrate, 12.4mmol of copper nitrate and 83.2mmol of urea are dissolved in deionized water.
5. 5. The process according to claim 1, wherein in the second step, the reaction is carried out by heating and stirring, then heating and maintaining the temperature, in particular, stirring in an environment of 65-85 ℃ for 0.5h, then heating and maintaining the reaction temperature to 95 ℃ for 3h.
6. 6. The preparation method of claim 1, wherein in the second step, the reaction product is centrifugally washed and dried, specifically, the reaction product is centrifugally washed and dried for 3-5 hours at 50-70 ℃; stirring, centrifugally washing a reaction product, and drying to obtain the nano enzyme, wherein the stirring is carried out for 0.5-1.5h at 85-95 ℃, and the centrifugal washing is carried out on the reaction product, and then the drying is carried out for 5-7h at 35-45 ℃ to obtain the nano enzyme.
7. 7. The method according to claim 1, wherein in the third step, indocyanine green and the bacterial targeting peptide UBI 29-41 are dissolved in ultrapure water in a mass ratio of 10:1 and stirred for 12-24 hours in the absence of light.
8. 8. The antibacterial photodynamic system for treating periodontitis by using the micro-environment response regulation ROS is characterized by being prepared by a preparation method of the antibacterial photodynamic system for treating periodontitis by using the micro-environment response regulation ROS according to any one of claims 1-7.
9. 9. The method of claim 8, wherein the product comprises a formulation, a medical material, or a medical device.

Description

Antibacterial photodynamic force for treating periodontitis by micro-environment response regulation and control of ROS (reactive oxygen species) and preparation method thereof Technical Field The invention belongs to the technical field of antibacterial photodynamic, and particularly relates to antibacterial photodynamic for treating periodontitis by micro-environment response regulation and control of ROS and a preparation method thereof. Background Periodontitis can cause loosening and even falling of teeth, and is also a risk-inducing factor of systemic diseases such as cardiovascular diseases, diabetes mellitus, hypertension and the like. Pathogenic bacteria in periodontal pockets of periodontitis can be transmitted through blood and other routes to cause infection of distant organs. The flow regulation shows that the periodontal health rate of adults in China is less than 10 percent. Therefore, it is important to effectively treat periodontitis and control pathogenic bacteria in periodontal pockets for a long time. Antibacterial photodynamic has been used clinically. However, the antibacterial photodynamic therapy used in the clinical periodontitis treatment has a plurality of defects that (1) the anoxic microenvironment in periodontal pockets and the easy self-aggregation characteristic of photosensitizers limit the efficiency of producing the ROS by the antibacterial photodynamic therapy, (2) the service life of the ROS is short from nanosecond to microsecond, the effective acting time of the antibacterial photodynamic therapy for exerting the antibacterial action is limited due to the limited diffusion distance, (3) the antibacterial photodynamic therapy can not generate ROS according to the characteristic responsiveness of the infected microenvironment (exert antibacterial action) and clear ROS (relieve tissue oxidative stress), and (4) the targeting of ROS bacteria generated by the antibacterial photodynamic therapy is insufficient to influence the antibacterial efficiency, and (5) the antibacterial photodynamic therapy is focused on the antibacterial therapy and the regulation capability of immune cells and osteoclasts in the periodontitis microenvironment is limited. Disclosure of Invention Aiming at the defects of the prior art, the invention provides an antibacterial photodynamic for treating periodontitis by micro-environmental response regulation and control of ROS and a preparation method thereof, and aims to solve the problems in the background art. In a first aspect, the invention provides a method for preparing an antimicrobial photodynamic for treating periodontitis by micro-environmental response regulation of ROS, comprising the following steps: Mixing sodium dodecyl benzene sulfonate, stannic chloride pentahydrate and L-cysteine into a mixed solution of ethylene glycol and water, stirring at room temperature in a dark place, performing hydrothermal reaction, cooling to room temperature after the reaction is finished, centrifugally collecting and washing to obtain a precipitate, and freeze-drying to obtain tin oxide sulfide; step two, dissolving zinc nitrate, copper nitrate and urea in deionized water, heating and stirring, then heating and maintaining the temperature, after the reaction is finished, centrifugally washing and drying a reaction product, and heating to 350-450 ℃ to obtain a precursor; Dissolving indocyanine green and bacterial targeting peptide UBI29-41 in ultrapure water according to the mass ratio of 10:1, stirring in a dark place, centrifuging and freeze-drying to obtain a combination product of photosensitizer indocyanine green and bacterial targeting peptide UBI29-41, adding nano enzyme and tin sulfide into an ultrapure water solution according to the mass ratio of 2:1, stirring overnight, centrifuging and freeze-drying to obtain nano enzyme-loaded tin sulfide oxide, stirring nano enzyme-loaded tin sulfide, dihydromyricetin and combination product in a dark place according to the mass ratio of 2:1:1 for overnight, centrifuging and washing the reaction product after the reaction is finished, and freeze-drying to obtain the antibacterial photodynamic. Further, in the first step, 22.4mmol of sodium dodecylbenzenesulfonate, 1.0mmol of stannic chloride pentahydrate, 8.0mmol of L-cysteine were mixed in a mixed solution of ethylene glycol and water. Further, in the first step, the temperature of the hydrothermal reaction is 150-170 ℃ and the time is 8-12h. Further, in step two, 10.1mmol of zinc nitrate, 12.4mmol of copper nitrate and 83.2mmol of urea were dissolved in deionized water. Further, in the second step, the mixture is heated and stirred, and then the temperature is raised and maintained, specifically, the mixture is stirred in an environment of 65-85 ℃ for 0.5h, and then the reaction temperature is raised to 95 ℃ and maintained for 3h. Further, in the second step, the reaction product is centrifugally washed and dried, specifically, the reaction product is cent