CN-121668742-B - Extraction equipment and method for high-purity rutin

CN121668742BCN 121668742 BCN121668742 BCN 121668742BCN-121668742-B

Abstract

The invention discloses extraction equipment and a method for high-purity rutin, which relate to the technical field of high-purity rutin extraction, and comprise equipment seats, wherein a chromatographic extraction mechanism is arranged on each equipment seat and consists of a first chromatographic column, a second chromatographic column and a third chromatographic column which are sequentially arranged from top to bottom, the first chromatographic column and the second chromatographic column are connected through a first connecting component, and the second chromatographic column and the third chromatographic column are connected through a second connecting component.

Inventors

LI GANG
GAN JING
JIAO XINXIN
YIN GUOWEI

Assignees

临朐天利生物制品有限公司

Dates

Publication Date: 20260512
Application Date: 20260210

Claims (6)

1. The extraction equipment for the high-purity rutin comprises an equipment seat (1), and is characterized in that a chromatographic extraction mechanism is arranged on the equipment seat (1), and comprises a first chromatographic column (3), a second chromatographic column (4) and a third chromatographic column (5) which are arranged from top to bottom; A first connecting component (9) is connected between the first chromatographic column (3) and the second chromatographic column (4), a second connecting component (10) is connected between the second chromatographic column (4) and the third chromatographic column (5), a material receiving seat (2) is fixedly arranged at the top end of the equipment seat (1), a discharging pipe (11) is connected between the third chromatographic column (5) and the material receiving seat (2), and a feeding pipe (8) is connected at the top end of the first chromatographic column (3); The first chromatographic column (3) is filled with macroporous adsorption resin filler, the second chromatographic column (4) is filled with polyamide filler, and the third chromatographic column (5) is filled with silica gel filler; The first connecting component (9) comprises an intermediate conveying pipe (18) fixedly arranged between the first chromatographic column (3) and the second chromatographic column (4), a plurality of guide vanes (19) are arranged in the intermediate conveying pipe (18), the guide vanes (19) are uniformly distributed along the circumference of the inner wall of the intermediate conveying pipe (18), the guide vanes (19) are axially arranged along the inner wall of the conveying pipe, one end of each guide vane (19) is fixedly connected with the intermediate conveying pipe (18), the other ends of the guide vanes (19) are fixedly connected with a rotating ring (26) together, and the rotating ring (26) is connected with an external rotary driving component; The external rotation driving assembly comprises an outer adjusting seat (20) fixedly arranged outside the middle conveying pipe (18), an adjusting chamber (23) is formed in the outer adjusting seat (20), a swinging rod (24) is arranged in the adjusting chamber (23), a rotating rod (25) penetrates through the swinging rod (24) and is connected with the swinging rod in a sliding mode, a guide bulge is arranged on the outer side of the rotating rod (25), an adjusting screw (21) is fixedly connected to the top end of the rotating rod (25), the adjusting screw (21) penetrates through the outer adjusting seat (20) and is in threaded connection with the outer adjusting seat, one end of the adjusting screw (21) located outside the outer adjusting seat (20) is coaxially and fixedly connected with an adjusting knob (22), a movable rod (27) is connected to the rotating ring (26) in a rotating mode, a slot is formed in the end portion of the movable rod (27), a spring (28) is fixedly connected to the slot, and the swinging rod (24) extends into the slot and is fixedly connected with the spring (28); The second connecting assembly (10) comprises module seats (29) assembled from top to bottom, a plurality of internal flow passages (31) are formed in each module seat (29), an upper flat cavity (32) and a lower flat cavity (33) are respectively formed in the top end and the bottom end of each module seat (29), and the plurality of module seats (29) are jointly penetrated and in threaded connection with a locking screw rod (30); One side of the discharging pipe (11) is fixedly connected with a sampling detection pipe (12), in the process that the solution passes through the discharging pipe (11), the sampling detection pipe (12) is used for sampling at regular time or quantitatively, the purity and concentration indexes of rutin in the solution are detected, whether the extraction process reaches an expected target or not is judged, if not, the angle of the guide vane (19) and the module seat (29) are adjusted according to the detection result, and the extraction is carried out again.
2. The extraction device for high-purity rutin according to claim 1, wherein the top ends of the first chromatographic column (3), the second chromatographic column (4) and the third chromatographic column (5) are fixedly connected with a top screen plate (13), and the bottom ends of the first chromatographic column (3), the second chromatographic column (4) and the third chromatographic column (5) are fixedly connected with a bottom screen plate (14).
3. The extraction device for high purity rutin according to claim 1, characterized in that said guide vane (19) is a plastic piece.
4. The extraction device for high-purity rutin according to claim 1, wherein the first chromatographic column (3), the second chromatographic column (4) and the third chromatographic column (5) are respectively provided with a glass cover.
5. The extraction device for high-purity rutin according to claim 1, wherein the first chromatographic column (3), the second chromatographic column (4) and the third chromatographic column (5) are all fixedly provided with a plurality of fixing lugs (7), the top end of the device seat (1) is fixedly provided with a fixing upright rod (6), and the fixing upright rod (6) penetrates through the plurality of fixing lugs (7) and is fixedly connected with the fixing lugs.
6. An extraction method using the extraction apparatus for high-purity rutin according to claim 2, characterized by comprising the steps of: Injecting a solution of high-purity rutin to be extracted into a first chromatographic column (3) through a feed pipe (8), selecting a first-stage filler (15), a second-stage filler (16) and a third-stage filler (17) according to the characteristics of the solution to be separated and the requirement of target rutin purity, filling the solution into the first chromatographic column (3), the second chromatographic column (4) and the third chromatographic column (5) respectively, and fixing the solution through a top sieve plate (13) and a bottom sieve plate (14); the solution is fully contacted with macroporous adsorption resin filler in a first chromatographic column (3), the macroporous adsorption resin filler is preliminarily enriched with rutin and part of macromolecular impurities are removed, and the solution flows to a middle conveying pipe (18) of a first connecting component (9) through a bottom sieve plate (14) under the action of gravity; Starting an external rotary driving assembly, rotating an adjusting knob (22) to drive an adjusting screw (21) to rotate, and as the adjusting screw (21) is in threaded connection with an adjusting outer seat (20), a rotating rod (25) slides up and down in an adjusting chamber (23) and rotates due to the rotation of the adjusting screw (21), a swinging rod (24) and a movable rod (27) are driven to swing, and then a rotating ring (26) is driven to rotate, and the rotating ring (26) drives a guide vane (19) to rotate under the action of the swinging rod (24), so that the angle of the guide vane (19) is changed, and a solution enters a second chromatographic column (4); The solution after flow guide adjustment enters a second chromatographic column (4), interacts with polyamide filler, further separates rutin and other flavonoid compounds, and flows into a module seat (29) of a second connecting component (10) through a bottom sieve plate (14) after the solution is fully chromatographed in the second chromatographic column (4); Different module seats (29) are selected and combined, the flow speed and the flow direction of the solution are regulated again by utilizing different internal flow passages (31) in the module seats (29), and when the solution flows in the module seats (29), the solution can be more uniformly distributed before entering the third analytical column (5) through the upper flat cavity (32) and the lower flat cavity (33); The solution after the previous two-stage chromatography and the runner adjustment enters a third chromatography column (5), contacts with silica gel filler, carries out fine purification, and obtains high-purity rutin solution, and the purified solution flows into a receiving seat (2) through a discharging pipe (11) to be collected.

Description

Extraction equipment and method for high-purity rutin Technical Field The invention relates to the technical field of high-purity rutin extraction, in particular to extraction equipment and method for high-purity rutin. Background In various fields of medicine, food, cosmetics and the like, the high-purity rutin has wide application due to excellent physiological activities such as antioxidation, anti-inflammatory, antivirus and the like. For example, in the medicine field, rutin can be used for treating cardiovascular and cerebrovascular diseases, assisting in reducing blood pressure and improving vascular permeability, in the food industry, the rutin is often used as a natural antioxidant, prolonging the shelf life of food and improving the nutritive value, and in the cosmetic industry, the rutin has the effects of resisting oxidation, whitening and the like, and is favored. Therefore, it is important to extract high-purity rutin efficiently and with high quality. Column chromatography is used as a core link in the high-purity rutin extraction process, and plays a decisive role in the purity and quality of the final product. However, currently employed column chromatography apparatus have a series of problems to be solved. Most conventional column chromatography devices rely on a single packed chromatography column. The single filler has obvious limitation, and is difficult to comprehensively cope with the complex impurity separation requirement in the rutin extraction process. For example, when only silica gel filler is used, although certain separation effect is achieved on certain polar impurities, the separation effect is poor on nonpolar impurities and other flavonoid compounds with similar properties to rutin, so that the purity of the product is limited, and the strict quality standard of high-purity rutin in the high-end market cannot be met. The control of the elution process is also not accurate enough. The flow rate, flow direction and mixing effect of the eluent and the additive are controlled mostly by experience of operators, and lack of accurate adjustment mechanism. Too fast flow rate may cause insufficient separation of rutin and impurities, and too slow flow rate may prolong the production period and increase the production cost. In addition, if the flow direction of the eluent in the chromatographic column is uneven, channeling is easy to occur, so that the packing in a partial area cannot fully play a role, and the separation efficiency is further reduced. Meanwhile, the eluent and the additive are insufficiently mixed, so that the elution condition cannot be effectively optimized, the desorption and separation effects of rutin are affected, and the product quality is unstable. In summary, a column chromatography device with an innovative structure is developed to overcome the problems, and has important practical significance for improving the overall level of extraction of the pure rutin. Disclosure of Invention The invention aims to provide the extraction equipment for the high-purity rutin, which improves the separation efficiency and purity of the rutin through the innovative design of a column chromatography structure, enhances the flexibility and adaptability of the operation of the equipment, is convenient for the maintenance and cleaning of the equipment and reduces the production cost. In order to achieve the above purpose, the present invention provides the following technical solutions: An extraction device for high-purity rutin comprises a device seat, wherein a chromatography extraction mechanism is arranged on the device seat. The chromatographic extraction mechanism consists of a first chromatographic column, a second chromatographic column and a third chromatographic column which are sequentially arranged from top to bottom. The first chromatographic column is connected with the second chromatographic column through a first connecting component, and the second chromatographic column is connected with the third chromatographic column through a second connecting component. The top of the equipment seat is fixedly provided with a receiving seat, a discharging pipe is connected between the third chromatographic column and the receiving seat, and the top of the first chromatographic column is connected with a feeding pipe. The first chromatographic column is filled with a first-level filler, the second chromatographic column is filled with a second-level filler, and the third chromatographic column is filled with a third-level filler. Specifically, the primary filler is macroporous adsorption resin filler, the secondary filler is polyamide filler, and the tertiary filler is silica gel filler. And, all fixedly connected with top sieve in first chromatographic column, second chromatographic column and the third layer chromatographic column, bottom all fixedly connected with bottom sieve. The top screen plate and the bottom screen plate are used for fixing the filler, and mean