CN-121694310-B - Pesticide composite drug carrying system and preparation and application thereof

Abstract

The invention provides a pesticide composite drug-carrying system, and preparation and application thereof, wherein the pesticide composite drug-carrying system comprises a pesticide, MOF-NH 2 and nano calcium carbonate, the pesticide is respectively loaded on the MOF-NH 2 and the nano calcium carbonate, and the nano calcium carbonate loaded with the pesticide is covered outside the MOF-NH 2 loaded with the pesticide. The pesticide composite drug-carrying system solves the problems that in the prior art, the pesticide-carrying MOF-NH 2 drug-carrying system is easy to collapse in skeleton in an acidic or high-salinity environment, and the drug-carrying performance and the pesticide slow-release effect are affected. The pesticide-containing composite drug carrying system has good application and popularization prospects.

Inventors

• ZHAO PENGYUE
• LI YUANYUAN
• HUANG QILIANG
• CAO LIDONG
• Zhou Lianxuan

Assignees

• 中国农业科学院植物保护研究所

Dates

Publication Date

20260508

Application Date

20260212

Claims (2)

1. 1. The preparation method of the pesticide composite drug-carrying system is characterized by comprising a pesticide, MOF-NH 2 and nano calcium carbonate, wherein the pesticide is respectively loaded on the MOF-NH 2 and the nano calcium carbonate, the nano calcium carbonate loaded with the pesticide is covered outside the MOF-NH 2 loaded with the pesticide, and the pesticide is fludioxonil, and the preparation method comprises the following steps: Step one), preparing a MOF-NH 2 carrier material; Step two), preparing a MOF-NH 2 pesticide carrying system loaded with pesticides; Step three), preparing a pesticide-containing composite drug-carrying system; wherein, the preparation of the compound pesticide carrying system containing pesticide in the step three) comprises the following steps: 1) Dispersing beta-cyclodextrin in water, and obtaining a solution A after ultrasonic dispersion; 2) Adding a MOF-NH 2 pesticide carrying system loaded with pesticide into water, and performing ultrasonic dispersion to obtain a suspension B; 3) Dropwise adding the suspension B into the solution A, dropwise adding a calcium chloride aqueous solution, continuously stirring after adding, adding an organic solvent containing fludioxonil, and dropwise adding a sodium carbonate aqueous solution after adding, wherein the organic solvent is one of methanol, ethanol, dichloromethane and acetonitrile, the mass concentration of fludioxonil in the organic solvent containing fludioxonil is 15-60mg/mL, the molar ratio of calcium chloride to sodium carbonate is 1 (0.5-3), and the mass concentration of beta-cyclodextrin in the solution A is 1-20mg/mL.
2. 2. The preparation method of the MOF-NH 2 carrying system loaded with pesticides according to claim 1, wherein the preparation of the MOF-NH 2 carrying system loaded with pesticides in the second step comprises the steps of weighing the MOF-NH 2 carrying material prepared in the first step, dissolving the MOF-NH 2 carrying material and the pesticides in an organic solvent, and stirring and reacting at room temperature, wherein the organic solvent is one of methanol, ethanol, dichloromethane and acetonitrile.

Description

Pesticide composite drug carrying system and preparation and application thereof Technical Field The invention relates to the technical field of pesticide dosage forms, in particular to a pesticide composite drug carrying system, and preparation and application thereof. Background With the continuous development of functional materials, slow release technology and processing technology, the controlled release technology has become a hotspot for pesticide formulation research. The control release technology can accurately regulate and control the time-space release behavior of the medicament according to the pest control demands, and achieve the control requirement of the whole growth period of crops by once soil application of the medicament to avoid repeated medicament application operation. In recent years, metal organic framework Materials (MOFs) are widely applied to the field of pesticide carriers due to the unique structural and functional advantages of MOFs. The applicant reports in the early stage CN114437365A that the nano drug-carrying particle framework material MOF-NH 2 can meet the load requirements of different pesticides. However, further research shows that the framework material still faces challenges such as limited stability in field application, for example, framework collapse easily occurs in an acidic or high-salinity environment, and drug carrying performance is affected. Research on better pesticide carrying systems and improvement of pesticide application effect are important research subjects in the field. Disclosure of Invention Aiming at the defects that in the prior art, a framework of a pesticide nano-drug-carrying particle framework material MOF-NH 2 serving as a pesticide carrier is easy to collapse in an acidic environment and the drug carrying performance of the pesticide nano-drug-carrying particle framework material is influenced when the pesticide nano-drug-carrying particle framework material MOF-NH 2 is applied in fields, the invention provides a composite drug carrying system of MOF-NH 2 and nano-calcium carbonate. In order to achieve the purpose, the invention covers the pesticide-loaded nano calcium carbonate drug-carrying system outside the original pesticide-loaded MOF-NH 2 system. Specifically, the invention adopts the following technical scheme: A composite pesticide carrying system containing pesticide comprises pesticide, MOF-NH 2 and nano calcium carbonate, wherein the pesticide is respectively loaded on the MOF-NH 2 and the nano calcium carbonate, and the nano calcium carbonate loaded with the pesticide is covered outside the MOF-NH 2 loaded with the pesticide. The invention also provides a preparation method of the MOF-NH 2 and nano calcium carbonate composite drug-carrying system. Advantageous effects 1. The MOF-NH 2 and nano calcium carbonate composite drug-carrying system overcomes the challenges of limited stability and the like of the MOF-NH 2 drug-carrying system in field application, such as easy occurrence of framework collapse in an acidic environment, influence on drug-carrying performance and the like. 2. The slow release effect of the MOF-NH 2 and nano calcium carbonate composite drug-carrying system is obviously better than that of the MOF-NH 2 drug-carrying system in the prior art. Drawings FIG. 1 is a schematic diagram of fludioxonil @ MOF-NH 2 @ CA drug delivery architecture. FIG. 2 is a scanning electron microscope photograph of (a, b) fludioxonil @ MOF-NH 2 @ CA drug carrier system, (c) MOF-NH 2 carrier material, (d) fludioxonil @ MOF-NH 2. FIG. 3 shows particle size distribution diagram of (a) nano calcium carbonate drug-carrying system, (b) MOF-NH 2 carrier material, (c) fludioxonil @ MOF-NH 2 @ CA drug-carrying system. FIG. 4 TEM-EDS elemental distribution of fludioxonil @ MOF-NH 2 @ CA. FIG. 5 different solvents were used to prepare fludioxonil @ MOF-NH 2 @ CA, (a) methanol, (b) dichloromethane, (c) acetonitrile, and (d) ethanol. FIG. 6 is a graph showing the molar ratios of fludioxonil @ MOF-NH 2 @ CA, (a) 1:1, (b) 1:2, (c) 1:3, and (d) 2:1. The fludioxonil @ MOF-NH 2 @ CA drug-loading system prepared by different active ingredient mass concentrations shown in figure 7 comprises (a) 15 mg/mL, (b) 30 mg/mL and (c) 60 mg/mL. FIG. 8 effect of different treatments (fludioxonil pro-drug, MOF-NH 2 @ CA, fludioxonil @ MOF-NH 2 @ CA) on wheat growth. Fig. 9 effects of different treatments (fludioxonil pro-drug, MOF-nh2@ca, fludioxonil@mof-nh2@ca) on wheat plant height, root length, fresh weight, dry weight, marked by Two-way ANOVA comparison test with a significance level of p≥0.05 indicating no significant difference, P < 0.05, P < 0.01, P < 0.001, P < 0.0001. FIG. 10 thermogravimetric analysis TGA (a) and differential thermogravimetric analysis DTG (b) of fludioxonil pro-drug, MOF-NH 2、MOF-NH2 @ CA and fludioxonil @ MOF-NH 2 @ CA. FIG. 11 release curves for fludioxonil pro-drug, fludioxonil @ MOF-NH 2 and fludioxonil @ MOF-NH 2 @ CA. FIG. 1