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CN-121695257-B - Application of protein AMBP in preparation of preparation for promoting hair follicle cell activity or preventing and treating alopecia

CN121695257BCN 121695257 BCN121695257 BCN 121695257BCN-121695257-B

Abstract

The invention belongs to the technical fields of biomedicine and cosmetics, and particularly relates to application of protein AMBP in preparation of a preparation for promoting hair follicle cell activity or preventing and treating alopecia. The invention discovers for the first time that the protein AMBP has the functions of regulating the activities of hair follicle papilla cells, hair matrix cells and outer root sheath cells, promoting the proliferation of the hair papilla cells, the hair matrix cells and the outer root sheath cells, improving the capability of the outer root sheath cells for scavenging active oxygen, promoting the cycle transition of hair follicle, and promoting the growth and thickening of hair. Thus, the protein AMBP can be used for preparing a preparation for treating alopecia.

Inventors

  • ZHANG LIN
  • ZHANG MIN
  • WANG XUEER
  • ZHANG LU

Assignees

  • 南方医科大学

Dates

Publication Date
20260508
Application Date
20260210

Claims (9)

  1. 1. The application of the protein AMBP in preparing a preparation for preventing and treating alopecia is characterized in that the protein AMBP is derived from human beings.
  2. 2. The use according to claim 1, characterized in that: the hair loss prevention and treatment is realized by promoting the activity of hair follicle cells; The hair follicle cell activity promotion is to promote proliferation of at least one of hair papilla cells, hair matrix cells and outer root sheath cells, and/or to enhance the ability of the outer root sheath cells to scavenge reactive oxygen species.
  3. 3. The use according to claim 1, characterized in that: the preparation is in the form of external liniment, external patch or injection.
  4. 4. A use according to claim 3, characterized in that: the external liniment is in the form of liquid, cream or ointment.
  5. 5. A use according to claim 3, characterized in that: The external patch is a percutaneous patch.
  6. 6. A use according to claim 3, characterized in that: The injection preparation is a skin injection preparation.
  7. 7. The use according to claim 6, characterized in that: the skin injection preparation is at least one of a microneedle injection preparation and a punctiform injection preparation.
  8. 8. The use according to any one of claims 1 to 7, characterized in that: the concentration of the protein AMBP in the preparation is 1-10000 ng/mL.
  9. 9. The use according to claim 8, characterized in that: The concentration of the protein AMBP in the preparation is 10-10000 ng/mL.

Description

Application of protein AMBP in preparation of preparation for promoting hair follicle cell activity or preventing and treating alopecia Technical Field The invention belongs to the technical fields of biomedicine and cosmetics, and particularly relates to application of protein AMBP in preparation of a preparation for promoting hair follicle cell activity or preventing and treating alopecia. Background At present, the alopecia treatment scheme mainly comprises schemes such as hair transplantation, oral medicines, emerging PRP injection and the like, the medicine treatment has a certain side effect, the effect of the medicine treatment can not meet the expectations of patients, the effect of the hair transplantation is closely related to the hair follicle state of the donor area of the patients, and the effect of the novel treatment scheme is different （ZHOU Y, et al. Advances in microneedles research based on promoting hair regrowth [J]. Journal of Controlled Release, 2023, 353: 965-74.）. from person to person, so that the searching and developing of novel components with prevention and treatment effects on hair loss have important significance. In mature individuals, hair follicles are generally not renewable, but rather exhibit a periodic cycle including anagen, catagen, and telogen. Anagen hair growth is faster, catagen hair stops growing, telogen hair falls off, and new hair regrows after entering the next anagen. The hair is divided into a hair shaft and a hair root. The hair follicle (hair follicle, HF), which is an accessory organ of skin, distributed in most skin of human body, is a complex tiny organ, the tissue structure is divided into two layers, the inner layer is an epithelial sheath and the outer layer is connective tissue sheath （HAMIDA OB, et al. Hair Regeneration Methods Using Cells Derived from Human Hair Follicles and Challenges to Overcome [J]. Cells, 2025, 14(1) : 7.）., the upper end of the epithelial sheath is continuous with the skin epidermis, the lower end is wrapped with the hair root and then is integrated with the hair root, the hair bulb is formed by expansion, the epithelial cells in the hair bulb are mainly hair matrix cells which can be differentiated to form the cells of the hair root and the epithelial sheath, thus the hair matrix cells are growing points of hair, the only source cells responsible for the actual synthesis and extension of hair, the activity and differentiation direction of the hair matrix cells directly determine the thickness, the length and the growth rate of hair, almost all types of alopecia are finally and directly or indirectly expressed as impaired hair matrix cell function, the activity is weakened （MA S, et al. Transcriptomic Analysis Reveals Candidate Ligand-Receptor Pairs and Signaling Networks Mediating Intercellular Communication between Hair Matrix Cells and Dermal Papilla Cells from Cashmere Goats [J]. Cells, 2023, 12(12) : 1645.）. Connective tissue at the bottom of hair bulb is protruded into hair bulb to form hair nipple, which contains abundant capillary vessel, nerve ending and hair nipple cell, which is the signal command center of hair follicle cycle, hair nipple cell is converted from resting stage to growing stage, hair follicle is induced to enter growing stage, alkaline phosphatase activity is enhanced (ALP) in hair follicle, a series of growth factors and signal molecules (such as Wnt, FGF, HGF, VEGF, etc.) are secreted, hair follicle stem cells and hair matrix cells are activated, new growth cycle （MEHTA A, MOTAVAF M, RAZA D, et al. Revolutionary Approaches to Hair Regrowth: Follicle Neogenesis, Wnt/ß-Catenin Signaling, and Emerging Therapies [J]. Cells, 2025, 14(11) : 779.）; is started, and the research of androgen alopecia patient's "bald area" human hair nipple cells are found to display aging phenotype earlier, and hair follicle inducing function （PAPPALARDO A, et al. Restoration of hair follicle inductive properties by depletion of senescent cells [J]. Aging Cell, 2025, 24(1) : e14353.）, is lost, thus regulating hair nipple cell activity and secretion capacity can promote hair cycle circulation and hair growth. Hair follicle outer root sheath cells are the primary reservoir of hair follicle stem cells that receive signals from hair follicle stem cells and convert them into commands for proliferation and differentiation, providing a source of cells （AGRAMUNT J, PARKE B, MENA S, et al. Mechanical stimulation of human hair follicle outer root sheath cultures activates adjacent sensory neurons [J]. Science Advances, 2023, 9(43) : eadh3273.）. for periodic hair follicle regeneration and thus how to regulate and maintain hair follicle cells, hair matrix cells, the viability of hair follicle outer root sheath cells being critical for the treatment of hair loss. Protein AMBP (Protein AMBP), also known as alpha 1-microglobulin/bicuculline precursor (alpha-1-microglobulin/bikunin precursor), is a "precursor plant" capable of producing