CN-121698950-B - Egg white protein source peptide with immunity hypofunction improving effect and application thereof

Abstract

The invention discloses an egg white protein source peptide with an effect of improving immune hypofunction and application thereof, and belongs to the technical field of small molecule peptides. The invention uses protease to carry out enzymolysis on egg white protein, intercepts enzymolysis products with molecular weight less than 1000 Da to prepare albumin peptide, wherein the albumin peptide contains tetrapeptide MSPF with the function of improving immune hypofunction. The animal model functional verification shows that the tetrapeptide MSPF and the albumin peptide containing the peptide segment can obviously improve the immune function deficiency, the macrophage number is increased by 62.94%, the neutrophil number is increased by 62.69%, and the interferon gamma content is increased by 76.11%, so that the immunity of the organism is enhanced. The food-borne bioactive peptide has high biological safety. Therefore, the tetrapeptide MSPF or the albumin peptide containing the peptide fragment can be applied to preparing medicines for improving the immune function or health-care foods for enhancing the immunity, and has good market prospect and application potential.

Inventors

• FENG FENGQIN
• DU JUAN
• ZHANG XI
• ZHAO MINJIE
• LIU HAIWANG
• BAO ZHONGYAN
• GAO YU

Assignees

• 杭州康源食品科技有限公司
• 浙江诺衍生物科技有限公司

Dates

Publication Date

20260508

Application Date

20260213

Claims (9)

1. 1. The application of tetrapeptide MSPF in preparing medicine for improving hypoimmunity or health food for enhancing immunity is characterized in that the amino acid sequence of tetrapeptide MSPF is Met-Ser-Pro-Phe.
2. 2. The use of claim 1, wherein the manifestation of immune dysfunction comprises a significant decrease in at least one of macrophage number, neutrophil number, interferon gamma content.
3. 3. The use according to claim 1, wherein the tetrapeptide MSPF is prepared by solid phase synthesis or is obtained by enzymatic hydrolysis of egg white proteins.
4. 4. The albumin peptide with the immunity improving effect is characterized by comprising tetrapeptides MSPF with the amino acid sequence of Met-Ser-Pro-Phe, and the preparation method of the albumin peptide comprises the following steps: (1) Mixing egg white protein powder with water according to the mass ratio of 1:15-20, and shearing to obtain an egg white protein powder solution; (2) Heating an egg white protein powder solution to 50 ℃, regulating the pH value to 8.0+/-0.2, adding alkaline protease accounting for 0.5-1.0% of the weight of the egg white protein powder, carrying out enzymolysis for 2-3 hours, adding papain accounting for 0.4-0.8% of the weight of the egg white protein powder, continuing to carry out enzymolysis for 4-6 hours, and then adding neutral protease accounting for 0.05-0.1% of the weight of the egg white protein powder, and continuing to carry out enzymolysis for 2-3 hours to obtain an enzymolysis solution; (3) Centrifuging the enzymolysis liquid at 4000-6000 r/min, collecting supernatant, filtering with membrane with molecular weight cut-off of 1000 Da, concentrating the filtrate, sterilizing, and drying to obtain albumin peptide.
5. 5. The use of the albumin peptide according to claim 4 for preparing a medicament for improving hypoimmunity or a health food for enhancing immunity.
6. 6. A pharmaceutical composition for improving immune dysfunction, comprising an effective amount of the albumin peptide of claim 4, and a pharmaceutically acceptable carrier.
7. 7. The pharmaceutical composition of claim 6, wherein the pharmaceutically acceptable carrier comprises one or more of a filler, a wetting agent, a disintegrant, a binder, or a lubricant.
8. 8. A health food for enhancing immunity, characterized in that it comprises the albumin peptide according to claim 4 as an active ingredient, and a pharmaceutically acceptable adjuvant.
9. 9. The health food according to claim 8, wherein the formulation of the health food is a tablet, a hard capsule, a soft capsule, an oral solution, a granule or a powder.

Description

Egg white protein source peptide with immunity hypofunction improving effect and application thereof Technical Field The invention relates to the technical field of small molecule peptides, in particular to an egg white protein source peptide with an effect of improving immune hypofunction and application thereof. Background The immune hypofunction refers to a pathological state of insufficient ability of the human immune system to resist pathogens, remove metabolic waste products and maintain internal environment stable. The immune system is composed of immune organs, immune cells and immune factors, is an important system for self-defense of human body, and the damage of the function of the immune system can lead to the increase of infection risks of bacteria, viruses and the like and influence the clearance capacity of abnormal cells. In recent years, the incidence of immunocompromised diseases caused by antitumor drugs, living pressure, and the like has increased year by year. Therefore, the immunity of the patients with low immunity is improved, and the method has important significance for preventing diseases. Bioactive peptides are peptide compounds derived from proteins, beneficial to the vital activities of organisms or having physiological effects, and have different molecular sizes, namely molecular polymers between amino acids and proteins, and are composed of as little as two amino acids and as much as tens of amino acids connected through peptide bonds. The bioactive peptide has wide sources and various activities, comprises antioxidation, antibiosis, immunoregulation and the like, has high edible safety, and has great potential application value in the food and pharmaceutical industry. Aiming at the development of bioactive peptide in the aspect of immunoregulation function, the current common immunoregulator is thymus peptide, and the thymus peptide which is commonly used in clinic is small molecular polypeptide which is discovered and purified from calf thymus and has non-specific immune effect, and can induce T cell differentiation and maturation, enhance cytokine production and enhance antibody response of B cells. Bestim (gamma-Glu-Trp) is a dipeptide molecule with immunoregulatory activity, and can inhibit adenylate cyclase activity （Kolobov AA, et al., Interaction of the synthetic immunomodulatory dipeptide bestim with murine macrophages and thymocytes. Bioorg Khim. 2008, 34(1):43-49.）. Chen Dongliang in macrophage and thymus cell membrane of mice, and the physical and chemical properties of peptide substances obtained by hydrolyzing chicken ovalbumin with protease and regulating immune function are studied, and the result shows that ovalbumin peptide has immune enhancement effect on the body of experimental mice (immune enhancement effect of ovalbumin peptide on the body of mice. Food science and technology, 2007: 219-223), but specific functional components are not analyzed in the study. The egg contains rich high-quality proteins, the proteins in egg white are mainly ovalbumin and egg globulin, 8 amino acids necessary for human bodies are contained in the egg white, the egg white is very similar to the composition of human body proteins, and the absorption rate of the human bodies to the egg proteins can be up to 98%. The egg white protein hydrolyzed by specific protease has various physical and chemical properties and biological activities, such as immunity enhancing, antioxidant and angiotensin converting enzyme activity inhibiting functions. Research shows that the mobility and absorption rate of small peptide substances from stomach to small intestine are superior to those of amino acids with the same components, and the small peptide with biological activity can be absorbed through intestine and can produce function regulating effect on organism. Therefore, the deep research on the immune enhancing functional components in the egg white protein peptide is necessary, and theoretical support basis is provided for the product development of the egg white protein enzymolysis product in the aspect of enhancing the immune effect. Disclosure of Invention The invention aims to provide a natural small molecular bioactive peptide with the effect of improving the immune hypofunction, which is applied to the development of medicines for improving the immune hypofunction or health-care foods for enhancing the immunity. In order to achieve the above purpose, the invention adopts the following technical scheme: The invention sequentially carries out biological enzymolysis on egg white protein powder by using alkaline protease, papain and neutral protease, and intercepts enzymolysis products with molecular weight smaller than 1000 Da to prepare egg white protein enzymolysis products. Analyzing peptide sequences in egg white proteolytic products by utilizing an LC-MS/MS peptide spectrum analysis technology, exploring the interaction between peptide fragments and a TNF-alpha receptor by adopting