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CN-121699030-B - Antiviral nidus Collocaliae polysaccharide active component for enriching combined sialic acid, preparation method and application thereof

CN121699030BCN 121699030 BCN121699030 BCN 121699030BCN-121699030-B

Abstract

The application relates to the technical field of food biology, and discloses an antiviral nidus Collocaliae polysaccharide active component for enriching combined sialic acid, a preparation method and application thereof. The weight average molecular weight of the bird's nest polysaccharide active component is 30 kDa-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, wherein the content of combined sialic acid is more than or equal to 24.0%, and the content of total sugar is more than or equal to 57.0%. The preparation method comprises pretreating nidus Collocaliae with boiling water bath, sequentially performing enzymolysis with alkaline protease and neutral protease, collecting supernatant, precipitating with ethanol, redissolving, performing enzymolysis with papain, inactivating enzyme, regulating ethanol concentration to 75% -85%, precipitating, and drying. The method can efficiently enrich the bird's nest polysaccharide and retain the combined sialic acid structure, and the obtained component has the functions of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

Inventors

  • FAN QUNYAN
  • LIU XUNCAI
  • GUO BAOZHONG
  • ZHANG XIAOJIANG
  • WANG NAN
  • Deng Fenghong
  • HU XIUTING

Assignees

  • 厦门市燕之屋丝浓生物科技有限公司

Dates

Publication Date
20260508
Application Date
20260212

Claims (13)

  1. 1. A method of preparing an antiviral nidus Collocaliae polysaccharide active component enriched in sialic acid in bound form, comprising: heating and pre-treating the bird's nest raw material to obtain a pre-treated substance, Sequentially carrying out alkaline protease enzymolysis treatment and neutral protease enzymolysis treatment on the pretreatment substance so as to obtain primary enzymolysis liquid; taking supernatant of the primary enzymolysis liquid, adding ethanol to enable the final concentration of the ethanol in the system to reach 75% -85%, standing at low temperature for precipitation, collecting precipitate, redissolving the precipitate, and then carrying out enzymolysis treatment of papain; inactivating enzyme of a papain enzymolysis treatment product to obtain a secondary enzymolysis solution, taking supernatant of the secondary enzymolysis solution, adding ethanol to enable the final concentration of the ethanol in a system to reach 75% -85%, standing at low temperature for precipitation, collecting the precipitation, and drying to obtain the antiviral nidus Collocaliae polysaccharide active component rich in combined nidus Collocaliae sialic acid; The heating pretreatment is realized by crushing the bird's nest raw material, soaking in water and performing boiling water bath treatment.
  2. 2. The method according to claim 1, wherein the alkaline protease enzymolysis treatment is carried out for 3-5 hours at a pH of 10.0-11.0 and a temperature of 45-55 ℃.
  3. 3. The method according to claim 1, wherein the neutral protease enzymolysis treatment is carried out for 3-5 hours at a pH of 6.5-7.5 and a temperature of 45-55 ℃.
  4. 4. The method according to claim 1, wherein the papain enzymolysis treatment is carried out for 4-6 hours at a pH of 6.0-7.0 and a temperature of 45-55 ℃.
  5. 5. The method according to claim 1, wherein the alkaline protease, the neutral protease and the papain are added in an amount of 10 x 10 4 ~90×10 4 U/g of raw material protein, each independently, in terms of enzyme-to-base ratio.
  6. 6. The method of claim 1, wherein the low temperature resting precipitation is at a temperature of 2 ℃ to 8 ℃ for a period of 8 to 16 hours.
  7. 7. An antiviral nidus Collocaliae polysaccharide active component rich in sialic acid in a combined state, which is characterized in that the nidus Collocaliae polysaccharide active component is prepared by the method of any one of claims 1-6.
  8. 8. The antiviral nidus Collocaliae polysaccharide active component rich in sialic acid in binding state of claim 7, wherein the weight average molecular weight of the nidus Collocaliae polysaccharide active component is 30 kDa-36 kDa, and the molecular weight distribution coefficient is 1.1-1.2; wherein the mass percentage of the combined sialic acid in the bird's nest polysaccharide active component is more than or equal to 24.0%, and the total sugar content is more than or equal to 57.0%; The total sugar content is the sum of N-acetylneuraminic acid, galactose, N-acetylglucosamine, N-acetylgalactosamine, mannose and fucose contents; The monosaccharide composition of the edible bird's nest polysaccharide active component comprises N-acetylneuraminic acid, galactose, N-acetylglucosamine, N-acetylgalactosamine, mannose and fucose, and contains Neu5Ac-Gal-GlcNAc or Neu5Ac-Gal glycan structural fragments.
  9. 9. The antiviral bird's nest polysaccharide active component rich in combined bird's nest sialic acid of claim 8, wherein the combined sialic acid is 24.0% -37.7% by mass.
  10. 10. The antiviral nidus Collocaliae polysaccharide active component enriched in sialic acid in conjugated form of claim 8, wherein the total sugar content is 57.0% -66.1%.
  11. 11. The use of the antiviral nidus Collocaliae polysaccharide active component rich in binding-state nidus Collocaliae sialic acid according to any one of claims 8-10 in preparing products with antioxidant function.
  12. 12. Use of an antiviral nidus Collocaliae polysaccharide active component enriched in binding-state nidus Collocaliae sialic acid according to any one of claims 8-10 for the preparation of a product for inhibiting escherichia coli, bacillus subtilis and/or having antiviral function.
  13. 13. Use of an antiviral nidus Collocaliae polysaccharide active component enriched in binding-state nidus Collocaliae sialic acid according to any one of claims 8-10 in the preparation of intestinal microecological regulator or prebiotic food.

Description

Antiviral nidus Collocaliae polysaccharide active component for enriching combined sialic acid, preparation method and application thereof Technical Field The application relates to the field of foods, in particular to an antiviral nidus Collocaliae polysaccharide active component for enriching combined sialic acid, a preparation method and application thereof. Background Bird's nest is a traditional and precious tonic, and its main active ingredients include protein (about 50%), carbohydrate (i.e., polysaccharide, about 20%), and sialic acid (about 10%). At present, under the influence of the concept of better activity of small molecules, research and development of deep processing products of bird's nest in the industry mainly focuses on the field of bird's nest peptides, namely, macromolecular proteins are converted into small molecular peptides through protease hydrolysis, so that the digestion and absorption rate of the products are improved, and polysaccharide components with larger molecular weight are often regarded as impurities and removed or not subjected to deep research. From the research of bird's nest processing technology, the traditional technology often neglects the targeted development and utilization of polysaccharide components. Carbohydrates (i.e., polysaccharides) in bird's nest exist mainly in the form of glycoproteins, wherein polysaccharide chains are used as connecting bridges, one end is connected with proteins, and the other end is connected with sialic acid, but in the conventional process of preparing bird's nest peptide, polysaccharide chains are often not effectively enriched as accompanying products, or the structures of polysaccharide chains are destroyed in the severe acid-base treatment process, so that bound sialic acid with important biological activity is lost in a large amount. Therefore, in order to maximize the nutritive value of the nidus Collocaliae, research on the polysaccharide active component of the nidus Collocaliae and the preparation method thereof is needed. Disclosure of Invention The application aims to overcome the defects in the prior art and provide an antiviral nidus Collocaliae polysaccharide active component rich in bonded nidus Collocaliae sialic acid. The application further provides a high-efficiency preparation method of the active component, and the method can obtain the high-purity and high-combined sialic acid content cubilose polysaccharide with high yield. The application also provides application of the active component in the aspects of resisting oxidization, resisting pathogenic microorganisms, regulating intestinal health as a prebiotic and the like. In order to achieve the above purpose, the present application adopts the following technical scheme: In a first aspect of the application, the application provides a method of preparing an antiviral nidus Collocaliae polysaccharide active component enriched in sialic acid in bound form. According to the embodiment of the application, the method comprises the steps of heating and pre-treating the raw material of the bird's nest to obtain a pre-treated matter, sequentially carrying out alkaline protease enzymolysis treatment and neutral protease enzymolysis treatment on the pre-treated matter to obtain a primary enzymolysis liquid, taking supernatant of the primary enzymolysis liquid, adding ethanol to enable the final concentration of the ethanol in a system to reach 75% -85%, standing at low temperature to precipitate, collecting precipitate, carrying out papain enzymolysis treatment on the precipitate after redissolution of the precipitate, inactivating enzyme of a product of the papain enzymolysis treatment to obtain a secondary enzymolysis liquid, taking supernatant of the secondary enzymolysis liquid, adding ethanol to enable the final concentration of the ethanol in the system to reach 75% -85%, standing at low temperature to precipitate, collecting precipitate and drying to obtain the antiviral bird's nest polysaccharide active component rich in combined bird's nest sialic acid. According to the method provided by the embodiment of the application, the antiviral nidus Collocaliae polysaccharide active component rich in the combined nidus Collocaliae sialic acid can be efficiently prepared and completely reserved. In some aspects of the application, the pretreatment is achieved by pulverizing the bird's nest material, soaking in water, and performing a boiling water bath treatment. In some aspects of the application, the alkaline protease enzymolysis treatment is realized by carrying out alkaline protease enzymolysis for 3-5 hours under the condition that the pH is 10.0-11.0 and the temperature is 45-55 ℃. In some aspects of the application, the neutral protease enzymolysis treatment is realized by carrying out neutral protease enzymolysis for 3-5 hours under the condition that the pH is 6.5-7.5 and the temperature is 45-55 ℃. In some aspects of the application, the papain enzymoly