CN-121699745-B - Stem cell culture device

CN121699745BCN 121699745 BCN121699745 BCN 121699745BCN-121699745-B

Abstract

The invention discloses a stem cell culture device, which belongs to the technical field of stem cells and comprises a sealed incubator and an injector, wherein an electric rotating table is arranged in the sealed incubator, an adjustable shaking assembly is arranged above the electric rotating table, a culture dish bearing frame and a culture dish limiting assembly are arranged on the adjustable shaking assembly, a uniform feeding assembly is arranged on the culture dish limiting assembly, the top end of the sealed incubator is in sealed sliding connection with a liquid injection pipe, the top end of the liquid injection pipe is provided with a sealed iris assembly, and a lifting assembly is arranged outside the sealed incubator. The culture dish realizes multi-dimensional periodic shaking through the adjustable shaking assembly, the culture dish limiting assembly and the uniform feeding assembly, promotes the stem cell wall-attached growth and the substance exchange, and maintains the tightness of the incubator all the time in the process of injecting the reagent by the aid of the sealing iris assembly and the injector, so that the sample is prevented from being exposed in the air, and the stability of the culture environment is ensured.

Inventors

ZHU SHUYU
ZHOU JING
ZHANG HONGRONG
LI CHANGQUAN
XIE YUHANG

Assignees

昆明市延安医院(昆明市干部疗养院)

Dates

Publication Date: 20260508
Application Date: 20260224

Claims (5)

1. The stem cell culture device comprises a sealed incubator (1) and an injector (12), and is characterized in that a connecting thread (1201) is arranged on the side wall of an injection port of the injector (12), an electric rotating table (2) is fixedly arranged at the bottom end inside the sealed incubator (1), and an adjustable shaking assembly (3) for dynamically improving the stem cell culture efficiency is arranged above the electric rotating table (2); The adjustable shaking assembly (3) is provided with a culture dish bearing frame (4) for bearing a plurality of culture dishes in a positioning manner and a culture dish limiting assembly (5) for pressing and limiting the culture dishes, a plurality of placing grooves (401) are formed in the upper surface of the culture dish bearing frame (4) along an annular array, a plurality of auxiliary feeding and mixing uniform feeding assemblies (6) are arranged on the culture dish limiting assembly (5), the uniform feeding assemblies (6) are corresponding to the placing grooves (401), the top ends of the sealed incubator (1) are in sealing sliding connection with a plurality of liquid injection pipes (8), sealing iris assemblies (9) matched with an injector (12) for auxiliary sealing feeding are arranged at the top ends of the liquid injection pipes (8), the liquid injection pipes (8) are matched with the uniform feeding assemblies (6), and lifting assemblies (7) for driving the liquid injection pipes (8) to lift are arranged outside the sealed incubator (1); The adjustable shaking assembly (3) comprises a servo speed reducing motor (301) fixed at the center of the top end of the sealed incubator (1) and a universal joint (308) fixed at the center of the top end of the electric rotating table (2), wherein an eccentric limiting shell (302) which is transversely and eccentrically arranged is fixedly connected to the output end of the servo speed reducing motor (301), a miniature electric push rod (303) is fixedly arranged in the eccentric limiting shell (302), a displacement block (304) is fixedly connected to one end of a movable rod of the miniature electric push rod (303) towards the axis, the side wall of the displacement block (304) is in sliding connection with the inner side wall of the eccentric limiting shell (302), a spherical connecting groove (3041) is formed in the bottom end of the displacement block (304), a threaded screw rod (307) and a telescopic rod (305) are sequentially fixedly connected to the top end of the universal joint (308) from bottom to top, the spherical joint (306) is in clamping connection with the spherical connecting groove (3041), a cross limiting groove (3071) is formed in the radial direction of the axis of the threaded screw rod (307), and the lower part of the threaded screw rod (307) is fixedly connected with the inner side wall of the center of the culture dish (4); The culture dish limiting assembly (5) comprises a displacement frame (501), wherein a cross limiting frame (503) is fixedly connected to the central side wall of the displacement frame (501), the side wall of the cross limiting frame (503) is slidably connected with the inner side wall of a cross limiting groove (3071), a plurality of mounting rings (502) corresponding to the position of the placing groove (401) are arranged on the displacement frame (501), a threaded cylinder (504) is rotatably connected to the top end of the displacement frame (501), the inner side wall of the threaded cylinder (504) is in threaded connection with the outer side wall of a threaded screw rod (307), a slave gear (507) is fixedly sleeved on the outer side wall of the threaded cylinder (504), a servo motor (505) and a protective shell (508) are fixedly arranged on the upper surface of the displacement frame (501), a master gear (506) is fixedly connected to the output end of the servo motor (505), the master gear (506) is meshed with the slave gear (507), and the servo motor (505), the master gear (506) and the slave gear (507) are all located in the protective shell (508); Even feeding subassembly (6) are circumference array fixed in guide plate (601) of collar (502) inner wall, fix reposition of redundant personnel pointed cone (602) at collar (502) inner wall, the surface of reposition of redundant personnel pointed cone (602) is arranged along the axle center and is equipped with a plurality of separation arch (6021), separation arch (6021) all are located between two adjacent guide plates (601), guide groove (6011) have radially been seted up to one side of guide plate (601) towards the centre of a circle, be separated by through-hole (603) between the edge of reposition of redundant personnel pointed cone (602) and guide groove (6011), the top of reposition of redundant personnel pointed cone (602) is fixed with mount (604), the inside wall rigid coupling of mount (604) has funnel (605), the bottom of funnel (605) is located the top of reposition of redundant personnel pointed cone (602), annotate liquid pipe (8) all are located the top of funnel (605), through-hole (6012) saw have all been seted up to the lower part lateral wall of guide plate (601) towards one side of centre of a circle, a plurality of fixed connection has between guide plate (601) bottom and guide groove (6011), a plurality of connection frame (606) have seted up on the surface of connection frame (6061).
2. The stem cell culture device according to claim 1, wherein the lifting assembly (7) comprises connecting seats (704) symmetrically fixed on the outer side wall of the sealed incubator (1), electric cylinders (701) are fixedly installed at the top ends of the connecting seats (704), the lengths of movable rods of the electric cylinders (701) and the liquid injection pipes (8) are not lower than the height of the sealed incubator (1), lifting plates (702) are fixedly connected at the top ends of the electric cylinders (701), a plurality of fixing holes (703) corresponding to the positions of the liquid injection pipes (8) are formed in the surface of the lifting plates (702), and the inner side walls of the fixing holes (703) are fixedly connected with the outer side walls of the liquid injection pipes (8).
3. The stem cell culture device of claim 1, wherein the sealed iris assembly (9) comprises a shell (901) fixed at the top end of the liquid injection tube (8), a first limit groove (9011) is formed in the inner side wall of the shell (901), an inner gear ring (902) is rotatably connected to the inner side wall of the first limit groove (9011), four transmission gears (903) which are uniformly distributed are in meshed connection with the inner side wall of the inner gear ring (902), the transmission gears (903) are rotatably arranged at the bottom end of the inner wall of the shell (901), four sealing wedge blocks (904) which are in sliding fit are driven between the transmission gears (903), teeth (9041) are formed in the outer side walls of the sealing wedge blocks (904), the teeth (9041) are meshed with the adjacent transmission gears (903), when the four sealing wedge blocks (904) are combined, rectangular limit protrusions (42) are arranged at the bottom ends of the inner side walls of the sealing wedge blocks (904) and the inner side walls (904) are connected with the two limit protrusions (12) which are in sliding fit with the inner side walls (905), the inner side wall of the connecting pipe (905) is provided with a thread groove (9051), the thread groove (9051) is in threaded fit with the connecting thread (1201), the side wall of the shell (901) is provided with a movable opening (9013), and a locking mechanism (14) for locking and limiting the inner gear ring (902) is arranged between the inner gear ring (902) and the shell (901).
4. A stem cell culture apparatus according to claim 3, wherein the locking mechanism (14) comprises a limiting member (1401) fixed on the outer side wall of the casing (901), a connecting member (1402) fixed on the outer side wall of the ring gear (902), the connecting member (1402) corresponds to the position of the limiting member (1401), a movable limiting plate (1403) is rotatably connected to the inner side wall of the connecting member (1402), the movable limiting plate (1403) is in limiting fit with the limiting member (1401), a magnet is fixed on the inner side wall of the limiting member (1401), and the movable limiting plate (1403) is made of a magnetically attractable metal material.
5. The stem cell culture device of claim 1, wherein a box opening (101) is formed in the side wall of the sealed incubator (1), a sliding groove (102) is formed in the inner side wall of the box opening (101), a glass sealing door (13) is slidably connected to the inner side wall of the sliding groove (102), a control panel (11) and a placing frame (10) are fixedly arranged on the outer wall of the sealed incubator (1), the control panel (11) is electrically connected with a servo speed reducing motor (301), a miniature electric push rod (303), a servo motor (505), an electric rotating table (2) and an electric cylinder body (701), and the injector (12) is placed inside the placing frame (10).

Description

Stem cell culture device Technical Field The invention belongs to the technical field of stem cells, and particularly relates to a stem cell culture device. Background Along with the continuous development of the current technology, the cell engineering application is very wide, the application has remarkable effect in both medical and pharmaceutical fields, and the cell culture is very important as the basis of the whole engineering in the cell engineering. Stem cells are a class of pluripotent cells with self-replicating capacity. Under certain conditions, it can differentiate into a variety of functional cells. Embryonic stem cells and adult stem cells are classified according to the developmental stage in which the stem cells are located. Stem cells are an insufficiently differentiated, yet immature cell with the potential function of regenerating various tissues and organs and the human body, and are called "universal cells" in the medical community. Thus, the culture of stem cells is particularly important. At present, most of the existing stem cell culture devices are static culture, so that stem cells in a culture dish cannot be uniformly contacted with nutrient substances and oxygen, the growth and propagation progress of the stem cells are slow, the culture efficiency of the stem cells is reduced, and in the single cell culture process, different essential nutrients (such as amino acids, vitamins and inorganic salts) required by each culture sample are required to be injected to maintain the growth, propagation and pluripotency of the stem cells, but in the operation process, an incubator is required to be switched on and off, the internal temperature of the incubator is easy to be severely changed frequently, samples are exposed to non-ideal environments, even cross contamination risks are caused, the stability of the whole culture environment is destroyed, and the growth state of the samples is further influenced. Disclosure of Invention The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a stem cell culture device. The technical scheme for solving the technical problems is that the stem cell culture device comprises a sealed incubator and an injector, wherein the side wall of an injection port of the injector is provided with connecting threads, an electric rotating table is fixedly arranged at the bottom end inside the sealed incubator, and an adjustable shaking assembly for dynamically improving the stem cell culture efficiency is arranged above the electric rotating table; The adjustable shake assembly is provided with a culture dish bearing frame for bearing a plurality of culture dishes in a positioning manner and a culture dish limiting assembly for pressing the culture dishes, a plurality of placing grooves are formed in the upper surface of the culture dish bearing frame along an annular array, a plurality of auxiliary feeding and mixing uniform feeding assemblies are arranged on the culture dish limiting assembly, the uniform feeding assemblies are corresponding to the placing grooves in position, the top end of the sealed incubator is in sealing sliding connection with a plurality of liquid injection pipes, the top ends of the liquid injection pipes are all provided with sealed iris assemblies matched with the auxiliary sealing feeding of the injector, the liquid injection pipes are matched with the uniform feeding assemblies, and a lifting assembly for driving the liquid injection pipes to lift is arranged outside the sealed incubator. Further, adjustable subassembly that rocks includes the servo gear motor of fixing at sealed incubator top center, fixes the universal joint at electric turntable top center, servo gear motor's output rigid coupling has the eccentric spacing shell of horizontal eccentric setting, the inside fixed mounting of eccentric spacing shell has miniature electric putter, miniature electric putter movable rod has the displacement piece towards the one end rigid coupling of axle center, the lateral wall and the inside wall sliding connection of eccentric spacing shell of displacement piece, spherical spread groove has been seted up to the displacement piece bottom, the top of universal joint is from last rigid coupling in proper order down has screw thread, telescopic link, the top of telescopic link is fixed with spherical joint, spherical joint and spherical spread groove block connection, cross spacing groove has radially been seted up in the axle center of screw thread screw, screw thread screw lower part lateral wall and culture dish carrier central inner wall fixed connection. Through the technical scheme, the combination of the eccentric limiting shell and the miniature electric push rod is driven by the servo gear motor, so that the composite motion track of the culture dish on the X/Y/Z three shafts, such as elliptical/spiral shaking, can accurately reproduce the shearing stress environment required