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CN-121730141-B - Planting and cultivating method for high-yield Dictyophora rubrovalvata

CN121730141BCN 121730141 BCN121730141 BCN 121730141BCN-121730141-B

Abstract

A process for culturing the high-yield Dictyophora rubrovalvata includes such steps as activating mycelium for 5-10 days, inoculating glucose-agar rejuvenation culture medium, culturing in dark, proportionally mixing wood dust, pretreated bamboo dust, auxiliary material, trichoderma inhibitor and eucommia bark leaf extract, regulating water content, sterilizing, bagging, inoculating to obtain full bag, inoculating to obtain seed bag, culturing, and culturing in fruiting mode of controlling temp, light and humidity by stages until the fungus skirt is opened and the fungus support is 15-20cm long. The process of the application improves strain stress resistance, reduces the strain pollution rate, ensures that the culture medium has excellent water retention and ventilation, has high carbon source utilization rate, reduces fruiting deformity rate and improves high-quality mushroom rate. Compared with the traditional method, the pollution rate of the mixed fungus is obviously reduced, the total yield of the Dictyophora rubrovalvata, the fungus skirt is complete, and the high-quality mushroom with thick stipe is greatly improved.

Inventors

  • YANG JINHUA
  • HE FEI

Assignees

  • 云南璟升农业有限公司

Dates

Publication Date
20260508
Application Date
20260225

Claims (7)

  1. 1. A planting and cultivating method of high-yield Dictyophora rubrovalvata is characterized by comprising the following steps of: The method comprises the steps of S1, inoculating the selected Dictyophora rubrovalvata strain to a PDA (personal digital assistant) culture medium, culturing at a constant temperature of 5-8 ℃ for 5-10 days to obtain an activated strain, inoculating the activated strain to a rejuvenation culture medium, culturing at a dark place of 5-8 ℃ for 15-20 days to obtain a rejuvenation strain, wherein the rejuvenation culture medium is specifically prepared by heating sterile deionized water to 60-70 ℃, adding glucose, stirring until the sterile deionized water is completely dissolved, adding agar, continuously heating and stirring until the agar is completely dissolved, adding the sterile deionized water to 1000mL, sterilizing and cooling to room temperature to obtain the rejuvenation culture medium; S2, preparing a strain culture medium, namely stirring wood dust, pretreated bamboo dust, auxiliary materials, a trichoderma inhibitor and a hypha-promoting growth factor for 30-60 minutes at room temperature, regulating the water content of a system to be 60-70%, naturally adjusting the pH value, sterilizing and bagging to obtain the strain culture medium, wherein the trichoderma inhibitor is at least one of trichoderma harzianum, 6-amyl-2H-pyran-2-one, trichoderma harzianum metabolic antibacterial peptide products and trichoderma harzianum metabolic product extracts, and the hypha-promoting growth factor is eucommia ulmoides leaf extracts; S3, inoculating and spawning, namely inoculating the rejuvenating strain in the step S1 to the strain culture medium in the step S2, keeping the ambient temperature at 22-25 ℃, keeping the air humidity at 70-75%, and ventilating for 2-3 times every day until the mycelia are full of bags, wherein the concentration of CO 2 is less than 1600 ppm; S4, fruiting management, namely after the mycelia are fully packed, treating the mycelia at a low temperature of 16-18 ℃ for 2-3 days, heating to 20-22 ℃ and culturing for 5-6 days to induce maturity, then promoting buds for 5-9 days, entering bud stage, culturing for 4-5 days to elongation stage, continuing to extend for 3-5 days, and harvesting when the fungus skirt is mature.
  2. 2. The planting and cultivating method of the high-yield Dictyophora rubrovalvata according to claim 1, wherein in the step S1, the rejuvenation culture medium comprises the following raw materials, by mass, 15-20g of glucose, 15-20g of agar and 1000mL of sterile deionized water.
  3. 3. The cultivation method for high-yield dictyophora rubrovolvata according to claim 1, wherein in the step S2, the pretreated bamboo scraps are specifically prepared by the following steps: Selecting bamboo scraps, crushing the bamboo scraps by using a crusher, regulating the water content of the system to 55-65% to obtain pre-wet bamboo scraps, piling the pre-wet bamboo scraps into a trapezoid pile, turning the outer bamboo scraps into the inside after 3-5 days of piling, regulating the water content of the bamboo scraps on the surface of the pile body to 55-65%, turning the pile once every 7-15 days, turning the pile for 3-4 times, and fermenting to obtain the pretreated bamboo scraps.
  4. 4. The planting and cultivating method of the high-yield Dictyophora rubrovalvata according to claim 1, wherein in the step S2, the auxiliary materials comprise, by mass, 15-20 parts of bran, 8-12 parts of corn flour, 3-5 parts of soybean meal powder, 0.5-1.5 parts of light calcium carbonate and 0.5-1.5 parts of gypsum powder.
  5. 5. The cultivation method for high-yield dictyophora rubrovolvata according to claim 1, wherein the eucommia ulmoides leaf extract is specifically prepared by the following steps: selecting dry eucommia ulmoides leaves, crushing and extracting for 2-3 hours by using an ethanol solution, then reflux-extracting the system for 2-3 times at 70-75 ℃ for 1.5-2 hours each time, collecting filtrate, and concentrating by rotary evaporation to obtain the eucommia ulmoides leaf extract.
  6. 6. The cultivation method for high-yield dictyophora rubrovolvata according to claim 1, wherein in the step S2, the mass ratio of wood chips to pretreated bamboo chips to auxiliary materials to trichoderma viride inhibitors to hypha-promoting growth factors is 40-50:20-30:30-35:0.1-0.3:0.01-0.2.
  7. 7. The cultivation method for high-yield Dictyophora rubrovalvata according to claim 1, wherein in the step S3, the inoculation amount is 2-2.5% of the weight of the strain culture medium.

Description

Planting and cultivating method for high-yield Dictyophora rubrovalvata Technical Field The invention belongs to the technical field of edible fungus planting, and particularly relates to a planting and cultivating method of high-yield dictyophora rubrovolvata. Background The Dictyophora rubrovalvata serving as a rare edible and medicinal fungus is rich in active ingredients, unique in flavor and vigorous in market demand, but the current large-scale planting is limited by multiple technical bottlenecks, and the industrial development is limited. In the prior art, the culture medium is mainly cultivated at the normal temperature of 20-25 ℃, stress domestication is not adopted, strain stress resistance is weak, field planting stagnation is easy to occur when the bamboo scraps of the culture medium are residual tannins and the environmental humidity fluctuates, the contamination rate of other miscellaneous bacteria such as trichoderma in the fungus growing period is always more than 10%, the rejuvenation culture medium is a PDA (personal digital assistant) culture medium with balanced nutrition, stress gene expression is induced without high-carbon low-nitrogen nutrition limitation, the utilization rate of a strain carbon source is low, and the quality is poor due to insufficient nutrition in the subsequent fruiting. When the culture medium of the Dictyophora rubrovalvata is prepared, bamboo scraps are only crushed and unfermented, tannic acid inhibits the activity of hyphase, cellulose is difficult to decompose, the carbon source supply efficiency is low, most of trichoderma inhibitors are chemical biological agents such as carbendazim, prochloraz and the like, food safety is easy to remain, and the mycelia of the Dictyophora rubrovalvata can be poisoned, most of growth promoting factors are universal, an enzyme system for decomposing wood materials by the mycelia cannot be activated in a targeted manner, the carbon source utilization rate is insufficient, and the growth of the mycelia is restricted. Based on the above, the application provides a planting and cultivating method for high-yield Dictyophora rubrovalvata. Disclosure of Invention The invention aims to provide a planting and cultivating method for high-yield Dictyophora rubrovalvata, and solves the problems in the prior art. The aim of the invention can be achieved by the following technical scheme: a planting and cultivating method of high-yield Dictyophora rubrovalvata comprises the following steps: S1, strain breeding and rejuvenation, namely transferring the selected Dictyophora rubrovalvata strain to a PDA (personal digital assistant) culture medium, culturing at a constant temperature of 5-8 ℃ for 5-10 days to obtain an activated strain, then inoculating the activated strain into a rejuvenation culture medium, and culturing at a dark place of 5-8 ℃ for 15-20 days to obtain the rejuvenation strain; S2, preparing a strain culture medium, namely stirring wood dust, pretreated bamboo dust, auxiliary materials, trichoderma inhibitors and hypha-promoting growth factors for 30-60 minutes at room temperature, regulating the water content of a system to 60-70%, naturally adjusting the pH value, sterilizing and bagging to obtain the strain culture medium; S3, inoculating and spawning, namely inoculating the rejuvenating strain in the step S1 to the strain culture medium in the step S2, keeping the ambient temperature at 22-25 ℃, keeping the air humidity at 70-75%, and ventilating for 2-3 times every day until the mycelia are full of bags, wherein the concentration of CO 2 is less than 1600 ppm; S4, fruiting management, namely after the mycelia are fully packed, treating the mycelia at a low temperature of 16-18 ℃ for 2-3 days, heating to 20-22 ℃ and culturing for 5-6 days to induce maturity, then transferring the mycelia into a mushroom forcing room, forcing buds for 5-9 days, entering bud period, culturing for 4-5 days to elongation period, continuing to extend for 3-5 days, and harvesting when the fungus skirt is fully opened, fungus holders are red and fungus handles are 15-20cm long. Further, the planting and cultivating method of the high-yield Dictyophora rubrovalvata comprises the following steps of: S1, strain breeding and rejuvenation, namely selecting hypha with white and thick strain and high growth speed, transferring the selected Dictyophora rubrovalvata strain to a PDA (personal digital assistant) culture medium, culturing at a constant temperature of 5-8 ℃ for 5-10 days to obtain an activated strain, and then inoculating the activated strain into a rejuvenation culture medium, and culturing at a dark state of 5-8 ℃ for 15-20 days to obtain the rejuvenation strain; S2, preparing a strain culture medium, namely stirring wood dust, pretreated bamboo dust, auxiliary materials, trichoderma inhibitors and hypha-promoting growth factors for 30-60 minutes at room temperature, regulating the water content of a system to 60-70%, naturally adjusti