CN-121754711-B - Composition containing recombinant human-derived type III collagen and antioxidant peptide and application thereof

Abstract

The invention relates to the technical field of medical dressings, in particular to a composition containing recombinant human type III collagen and antioxidant peptide and application thereof. According to the invention, the novel antioxidant peptide LR and the recombinant human type III collagen COL3A1 are used in a compounding way according to a certain proportion, and an optimal composition is selected through in-vitro antioxidant index detection and repair experiments on UVB damaged cells. The final result shows that the composition provided by the invention can achieve the effects of antioxidation and skin repair. The composition provided by the invention provides a new thought for improving the antioxidant activity and skin repair effect of the III type collagen.

Inventors

• HAO LINLIN
• SU TONG
• LIU DONGYUE
• XIAO XINGYU
• FANG JIAYUAN

Assignees

• 吉林省国大生物工程有限公司

Dates

Publication Date

20260512

Application Date

20260304

Claims (7)

1. 1. The application of the composition in preparing a product with antioxidant and skin repairing effects is characterized in that the composition consists of recombinant human type III collagen COL3A1 and antioxidant peptide LR, wherein the amino acid sequence of the recombinant human type III collagen COL3A1 is shown as SEQ ID NO.2, the amino acid sequence of the antioxidant peptide LR is shown as SEQ ID NO.15, and the mass ratio of the recombinant human type III collagen COL3A1 to the antioxidant peptide LR in the composition is 1:19.
2. 2. The use according to claim 1, wherein the method of preparing the composition comprises the step of mixing the recombinant human type III collagen COL3A1 and the antioxidant peptide LR.
3. 3. A product with antioxidant and skin repairing effects is characterized by comprising recombinant human type III collagen COL3A1 and antioxidant peptide LR, wherein the amino acid sequence of the recombinant human type III collagen COL3A1 is shown as SEQ ID NO.2, the amino acid sequence of the antioxidant peptide LR is shown as SEQ ID NO.15, and the mass ratio of the recombinant human type III collagen COL3A1 to the antioxidant peptide LR in the composition is 1:19.
4. 4. A product according to claim 3, wherein the product further comprises an auxiliary material.
5. 5. The product of claim 4, wherein the product comprises a medical dressing.
6. 6. The product according to claim 4, wherein the dosage form of the product comprises one or more of an aqueous solution, an oil solution, an emulsion, a cream, a paste and a gel.
7. 7. The application of the antioxidant peptide LR in preparing products with antioxidant and skin repairing effects is characterized in that the amino acid sequence of the antioxidant peptide LR is shown as SEQ ID NO. 15.

Description

Composition containing recombinant human-derived type III collagen and antioxidant peptide and application thereof Technical Field The invention relates to the technical field of medical dressings, in particular to a composition containing recombinant human type III collagen and antioxidant peptide and application thereof. Background In recent years, with the improvement of the quality of life and the pursuit of beauty, therapeutic agents for reducing pigmentation and anti-aging and wrinkle removal have attracted great interest in the clinical and cosmetic fields. Commercial antioxidant and whitening agents, including hydroquinone, arbutin, kojic acid, vitamin C and some botanicals, have been reported to be disabled due to various adverse side effects (such as contact dermatitis, irritation, white skin disease, hypopigmentation and ocher) that can occur with prolonged use. Antioxidant peptides, which are one of the emerging antioxidants, have been attracting attention in the food and health fields in recent years due to their nature, effectiveness, bioavailability, suitability, and the like. The research shows that the antioxidant peptide has not only the function of eliminating free radical in vivo, but also obvious protection effect on lipid peroxidation induced by free radical, and the action mechanism of the antioxidant peptide also comprises the steps of providing hydrogen for antioxidant enzyme, chelating metal ions and the like. Collagen is the protein with the highest content in the human body, is distributed in various organs and tissues of the human body, and has the highest content in the dermis layer of the skin. The skin of young people is smooth, full and elastic, and the main reason is that the collagen is relatively abundant. Compared with animal collagen, the collagen obtained by recombinant expression has high activity, good hydrophilicity and water retention, can improve skin metabolism, has no virus residue, no immunogenicity and no endotoxin, and can be used for a long time and is safe. The III type collagen has very wide application in daily life, and forms medical dressing mainly comprising sponge dressing, film dressing, gel dressing and the like with the functions of treating wound infection, promoting tissue regeneration and closing wound. The recombinant human III type collagen produced by genetic engineering technology is a new method in recent years, and at present, the recombinant human III type collagen is mostly expressed by small fragments, and the full-length expression of the recombinant human III type collagen needs hydroxylation of proline hydroxylase, but the common bacterial expression system and yeast expression system do not contain proline hydroxylase, so that the stability of the product obtained by recombinant expression is very low, the product is extremely easy to degrade, and the use of the recombinant collagen is greatly limited. In view of the above, it is important to provide a stable composition of human type III collagen and antioxidant peptide having both antioxidant and skin repairing effects. Disclosure of Invention The present invention is directed to a composition comprising recombinant human type III collagen and an antioxidant peptide and its use to solve the above-mentioned problems of the prior art. In order to achieve the above object, the present invention provides the following solutions: The invention provides a composition containing recombinant human type III collagen and antioxidant peptide, which comprises recombinant human type III collagen COL3A1 and antioxidant peptide LR, wherein the amino acid sequence of the recombinant human type III collagen COL3A1 is shown as SEQ ID NO.2, and the amino acid sequence of the antioxidant peptide LR is shown as SEQ ID NO. 15. Optionally, the mass ratio of the recombinant human type III collagen COL3A1 to the antioxidant peptide LR in the composition is 1 (13.9-19). Optionally, the mass ratio of the recombinant human type III collagen COL3A1 to the antioxidant peptide LR in the composition is 1:19. The invention provides a preparation method of the composition, which comprises the step of mixing the recombinant human type III collagen COL3A1 and the antioxidant peptide LR. The invention provides application of the composition in preparing products with antioxidant and skin repairing effects. The invention provides a product with antioxidant and skin repairing effects, which comprises the composition. Optionally, the product further comprises an auxiliary material. Optionally, the product comprises a medical dressing. Optionally, the dosage form of the product comprises one or more of water agent, oil agent, emulsion, cream, ointment and gel. The invention provides application of antioxidant peptide LR in preparation of products with antioxidant and skin repairing effects, and the amino acid sequence of the antioxidant peptide LR is shown as SEQ ID NO. 15. The invention discloses the following technical