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CN-121759371-B - Nitrogen-philic bacteria for wastewater treatment and culture method and application thereof

CN121759371BCN 121759371 BCN121759371 BCN 121759371BCN-121759371-B

Abstract

The invention relates to the field of microorganisms, in particular to azophilic bacteria for wastewater treatment and a culture method and application thereof. The azophilic bacteria are Paracoccus sp.HF-E2025009, and are preserved in China general microbiological culture Collection center (CGMCC) No.36935 in the year 2025, month 12 and 8. The strain can keep stable ammoxidation activity under the conditions of high ammonia nitrogen and high sulfate, can be started in a short period of time, has obvious ammonia nitrogen removal effect, realizes synchronous denitrification and partial removal of organic matters and sulfate, does not need a large amount of additional organic carbon or expensive chemical reagents in the subsequent denitrification step, reduces the overall cost, has simple process addition and installation, does not need large-scale reconstruction of the existing treatment device, and has wide prospect in the aspect of wastewater treatment.

Inventors

  • Kou Qingfen
  • ZHANG FENGZHAO
  • PENG CHAO

Assignees

  • 山东环发科技开发有限公司

Dates

Publication Date
20260508
Application Date
20260304

Claims (10)

  1. 1. The azophilic bacteria for treating waste water features that the azophilic bacteria is Paracoccus sp.HF-E2025009 and HF-E2025009 is preserved in China general microbiological culture Collection center with the preservation number of CGMCC No.36935 in 2025 and 12-8.
  2. 2. A microbial culture, characterized in that the culture comprises the azophilic bacteria of claim 1.
  3. 3. A microbial agent comprising an effective amount of the azophilic bacterium of claim 1 or the microbial culture of claim 2, and a carrier.
  4. 4. The microbial agent according to claim 3, wherein the microbial agent is a freeze-dried powder, granule or concentrate, and the carrier comprises at least one of activated carbon, porous ceramic, polyvinyl alcohol gel and diatomite.
  5. 5. The microbial agent according to claim 4, further comprising at least one other functional microorganism selected from the group consisting of nitrifying bacteria, denitrifying bacteria and phosphorus accumulating bacteria.
  6. 6. A method of wastewater treatment, characterized in that the method comprises adding an effective amount of the azophilic bacterium according to claim 1, or the microbial culture according to claim 2, or the microbial inoculant according to any one of claims 3-5, to the wastewater to be treated.
  7. 7. The method of claim 6, wherein the effective amount is 50 to 600ppm.
  8. 8. The method of claim 6, wherein the wastewater is of a type selected from at least one of domestic sewage, aquaculture wastewater, food processing wastewater, landfill leachate.
  9. 9. Use of the azophilic bacterium of claim 1, or the microbial culture of claim 2, or the microbial inoculant of any one of claims 3-5, in any one of the following: A1. The application in preparing products for denitrification of wastewater; A2. The application in the preparation of products for reducing the organic matter content in wastewater; A3. Use in the preparation of a product for reducing the sulphate content of waste water.
  10. 10. A wastewater treatment system, characterized in that the system comprises a bio-enhancement module filled with a biofilm of the azophilic bacteria of claim 1 or the microbial culture of claim 2, or the microbial inoculant of any one of claims 3-5.

Description

Nitrogen-philic bacteria for wastewater treatment and culture method and application thereof Technical Field The invention relates to the field of microorganisms, in particular to azophilic bacteria for wastewater treatment and a culture method and application thereof. Background With the rapid development of industrial production, high ammonia nitrogen wastewater discharged by industries such as chemical industry, printing and dyeing, papermaking, cultivation, landfill leachate and the like is increased, ammonia nitrogen is taken as one of main pollutants of a water body, and excessive discharge can cause eutrophication of the water body, so that a series of environmental problems such as blue algae burst, dissolved oxygen reduction and the like are caused, and ecological environment and human health are seriously threatened. Therefore, the effective treatment of high ammonia nitrogen wastewater has become an important issue in the field of environmental protection. At present, the wastewater denitrification mainly adopts a biological denitrification technology, and the core of the technology is that ammonia nitrogen is converted into nitrogen through ammoxidation, nitrosation, nitrification and denitrification of microorganisms. The traditional biological denitrification process depends on the synergistic effect of nitrifying bacteria and denitrifying bacteria, but has a plurality of limitations that on one hand, nitrifying bacteria grow slowly and are sensitive to environmental conditions, high sulfate wastewater has biotoxicity, proliferation speed and strain activity are easy to be inhibited under high ammonia nitrogen and high sulfate concentration, and on the other hand, a large amount of organic carbon is needed as an electron donor in the traditional denitrification process, and organic carbon sources such as methanol, glucose and the like are additionally added to high ammonia nitrogen wastewater with low organic carbon content, so that the treatment cost is obviously increased, and secondary pollution is easy to cause. In addition, the traditional process has long starting period and high modification requirement on the existing treatment device, and limits the application effect of the traditional process in the treatment of high ammonia nitrogen wastewater. CN107760622a discloses a strain of paracoccus denitrificans, which can grow by using organic carbon, ammonia nitrogen and other elements in high ammonia nitrogen wastewater, thereby realizing reduction of COD and ammonia nitrogen in the high ammonia nitrogen wastewater, however, the strain does not involve sulfate treatment. However, if the high sulfate in the industrial wastewater (such as mining, pharmacy and papermaking wastewater) needs to be removed, the chemical precipitation method (such as adding barium salt or calcium salt to generate barium sulfate/calcium sulfate precipitation) or the membrane separation method (such as reverse osmosis) needs to be adopted for separate pretreatment. Disclosure of Invention Aiming at the defects of the prior art, the invention provides the high-efficiency azotobacter which is resistant to high ammonia nitrogen and high sulfate, is adaptive to low-organic carbon environment and has strong process compatibility, and has important significance for optimizing biological denitrification process, reducing treatment cost and improving high ammonia nitrogen wastewater treatment effect. In a first aspect of the present invention, there is provided a azophilic bacterium for wastewater treatment, wherein the azophilic bacterium is Paracoccus sp.HF-E2025009, and the azophilic bacterium HF-E2025009 is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) at a position of North Star West Song No. 1, a position of Beijing, and a strain number of CGMCC No.36935. Further, the azotobacter can maintain stable ammoxidation activity in an environment with ammonia nitrogen concentration reaching 500 mg/L, and can withstand a high sulfate environment. Further, the strain can efficiently convert ammonia nitrogen into NO 2-, and under the condition of low oxygen or hypoxia, the strain can further convert NO 2- into nitrogen and can synchronously remove part of sulfate under the condition of low organic carbon. Further, the strain is facultative/aerobic bacteria, the optimal growth pH is 6.9-7.6 (the pH value application range is 6.8-8.5), the optimal culture temperature is 30 ℃ (the temperature application range is 25 ℃ -36 ℃), and further, the 16S rRNA gene sequence of the azophilic bacteria is shown as SEQ ID NO. 1. Further, the strain is still effective in removing ammonia nitrogen and total nitrogen under one or more of the following conditions: (a) Ammonia nitrogen concentration is less than or equal to 500mg/L; (b) High sulfate tolerance less than or equal to 1500mg/L; (c) The temperature is 25-36 ℃; (d) The pH value is 6.8-8.5. In a second aspe