CN-121800873-B - Anti-atherosclerosis polypeptide of Buthus martensii Karsch and application thereof

CN121800873BCN 121800873 BCN121800873 BCN 121800873BCN-121800873-B

Abstract

The invention discloses an anti-atherosclerosis polypeptide of Buthus martensii Karsch and application thereof, belonging to the technical field of traditional Chinese medicine polypeptides. The anti-atherosclerosis polypeptide of the Buthus martensii Karsch provided by the invention is one of peptide fragment 1, peptide fragment 2, peptide fragment 3, peptide fragment 4, peptide fragment 5, peptide fragment 6 and peptide fragment 7, and the amino acid sequences are respectively shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6 and SEQ ID NO. 7. The anti-atherosclerosis polypeptide obtained by screening can obviously reduce oxidative damage, reduce lipid deposition, especially the peptide segment 5, provide theoretical basis for medicinal development of the anti-atherosclerosis peptide of the Buthus martensii Karsch, and reveal potential anti-atherosclerosis mechanism.

Inventors

HU JINGQING
Zong Wentian
GENG ZHIRONG
ZHANG XIAOHONG

Assignees

南京中医药大学

Dates

Publication Date: 20260512
Application Date: 20260311

Claims (4)

1. The anti-atherosclerosis polypeptide is characterized by being one of peptide segment 4, peptide segment 5 and peptide segment 6; The amino acid sequence of the peptide fragment 4 is shown as SEQ ID NO. 4; The amino acid sequence of the peptide fragment 5 is shown as SEQ ID NO. 5; the amino acid sequence of the peptide fragment 6 is shown as SEQ ID NO. 6.
2. The anti-atherosclerosis polypeptide of the Buthus martensii Karsch according to claim 1, wherein the anti-atherosclerosis polypeptide is synthesized by solid phase synthesis.
3. An application of the anti-atherosclerosis polypeptide of the Buthus martensii Karsch in preparing anti-atherosclerosis drugs, wherein the anti-atherosclerosis polypeptide of the Buthus martensii Karsch is one of peptide segment 4, peptide segment 5 and peptide segment 6.
4. The use according to claim 3, wherein the anti-atherosclerosis polypeptide of Buthus martensii Karsch is peptide 5.

Description

Anti-atherosclerosis polypeptide of Buthus martensii Karsch and application thereof Technical Field The invention belongs to the technical field of traditional Chinese medicine polypeptides, and particularly relates to an anti-atherosclerosis polypeptide of Buthus martensii Karsch and application thereof. Background Atherosclerosis is an inflammatory disease that causes arterial stenosis with elevated blood lipids, and common lipid-lowering therapies lack the effect of controlling the progression of atherosclerotic plaques, in which inflammation plays a major driving role, especially in oxidative stress and lipid deposition mediated inflammation. Development of anti-inflammatory drugs against oxidative stress and lipid deposition may be an effective way to reverse atherosclerosis. As a traditional Chinese medicinal material, the Buthus martensii Karsch has the effects of counteracting toxic substances, resolving hard mass, calming endogenous wind, relieving spasm, dredging collaterals and relieving pain, and modern medical research focuses on protein and polypeptide active ingredients, and the research shows that the polypeptide in the Buthus martensii Karsch has the effects of resisting inflammation, resisting bacteria, protecting nerves, promoting cell proliferation and the like. May be used to control the inflammatory response of atherosclerosis and exert an anti-atherosclerosis effect. The polypeptide is a kind of bioactive small molecules formed by connecting 2-10 amino acids through peptide bonds, and has important value in drug development and biomedical research. In particular, the polypeptide has small molecular weight and good water solubility, can be directly absorbed and utilized by intestinal tracts, and does not need to undergo the complex digestion process of protein. For people with weak gastrointestinal functions, the polypeptide can effectively improve the protein utilization rate and improve the malnutrition state. Clinically, it is often used for postoperative rehabilitation, and nutritional support for elderly and patients with digestive system diseases. The anti-inflammatory effect combined with the Buthus martensii Karsch has the prospect of developing anti-atherosclerosis polypeptide, but the structure and the function of the Buthus martensii Karsch single polypeptide are not well known at present, which limits the clinical application and the molecular mechanism exploration of the Buthus martensii Karsch single polypeptide. Disclosure of Invention Aiming at the problems in the prior art, the invention provides an anti-atherosclerosis polypeptide of Buthus martensii Karsch and application thereof, provides a theoretical basis for development of anti-atherosclerosis polypeptide drugs, and reveals potential anti-oxidation and anti-lipid deposition mechanisms. The invention is realized by the following technical scheme: In a first aspect of the present invention, an anti-atherosclerosis polypeptide of the Buthus martensii Karsch is provided, wherein the anti-atherosclerosis polypeptide of the Buthus martensii Karsch is one of peptide 1, peptide 2, peptide 3, peptide 4, peptide 5, peptide 6 and peptide 7; the amino acid sequence of the peptide fragment 1 is shown as SEQ ID NO. 1; the amino acid sequence of the peptide fragment 2 is shown as SEQ ID NO. 2; the amino acid sequence of the peptide fragment 3 is shown as SEQ ID NO. 3; The amino acid sequence of the peptide fragment 4 is shown as SEQ ID NO. 4; The amino acid sequence of the peptide fragment 5 is shown as SEQ ID NO. 5; The amino acid sequence of the peptide fragment 6 is shown as SEQ ID NO. 6; the amino acid sequence of the peptide fragment 7 is shown as SEQ ID NO. 7. Further, the anti-atherosclerosis polypeptide of the Buthus martensii Karsch is synthesized by adopting a solid-phase synthesis method. In a second aspect, the invention provides an application of the Buthus martensii Karsch anti-atherosclerosis polypeptide in preparing anti-atherosclerosis drugs, wherein the Buthus martensii Karsch anti-atherosclerosis polypeptide is one of peptide segment 1, peptide segment 2, peptide segment 3, peptide segment 4, peptide segment 5, peptide segment 6 and peptide segment 7. Further, the anti-atherosclerosis polypeptide of the Buthus martensii Karsch is peptide 5. Compared with the prior art, the invention has the following beneficial effects: The anti-atherosclerosis polypeptide peptide fragment (especially peptide fragment 5) obtained by screening can obviously reduce oxidative damage and lipid deposition, provides theoretical basis for medicinal development of the anti-atherosclerosis peptide of the Buthus martensii Karsch, and reveals a potential anti-atherosclerosis mechanism. Drawings FIG. 1 is a diagram of antioxidant effect fluorescent probes of 10 candidate polypeptides on RAW264.7 cell model; FIG. 2 is a graph showing the fluorescence statistics of 10 candidate polypeptides on a RAW264.7 cell model MCF probe; FIG. 3 is a