Search

CN-121819973-B - Automatic pipetting workstation

CN121819973BCN 121819973 BCN121819973 BCN 121819973BCN-121819973-B

Abstract

The invention discloses an automatic pipetting workstation, which belongs to the technical field of pipetting equipment and comprises a plurality of single pipetting modules which are arranged side by side, wherein each single pipetting module comprises a suction head module, a raw material platform, a linear conveying module, a suction head collecting module, a movable positioning module and a pipetting module, and each pipetting module comprises a nozzle opening. This pipetting station is high flux liquid preparation modularization assembly line-this assembly line wholly adopts the modularized design, and every module is inside to have a plurality of liquid feeding station, and the corresponding pipetting module that is equipped with equal quantity can accomplish the interpolation of multiple different solution, and the centrifuging tube realizes intermittent type nature progressive through sharp transport module, is progressively advanced to next work module one by one, and until accomplish the interpolation of all kinds of solutions, compares in traditional pipetting station, and this pipetting station adopts "modularization + assembly line" form operation, can realize that all stations work simultaneously, has solved the problem that traditional pipetting station repeatedly moves the inefficiency that leads to.

Inventors

  • HE YONG
  • Hou Yike
  • SUN YUAN
  • XU WANQING
  • WANG XINKAI

Assignees

  • 良渚实验室

Dates

Publication Date
20260508
Application Date
20260310

Claims (9)

  1. 1. An automatic pipetting workstation is characterized by comprising a plurality of single pipetting modules (100) which are arranged side by side, wherein each single pipetting module (100) comprises a suction head module (400), a raw material platform (500), a linear conveying module (600), a suction head collecting module (700), a mobile positioning module (200) and a pipetting module (300) arranged on the mobile positioning module (200); the suction head module (400) comprises first linear motion mechanisms (404) with the same number as that of the pipetting modules (300), and each first linear motion mechanism (404) is provided with a universal base table; The universal base table comprises a base part, and a following moving table (402) is arranged above the base part; The base part comprises a correcting piece which is arranged in the inner cavity of the base part and is connected with the following moving table (402), and the correcting piece comprises a universal rolling device which can enable the following moving table (402) to roll and translate and a magnetic resetting device which can perform magnetic resetting treatment on the translated following moving table (402); the base part comprises a base platform (405) connected to the mover seat in the first linear motion mechanism (404); the top of the base platform (405) is connected with a middle box seat (401); the universal rolling device comprises a T-shaped movable seat (406); the bottom of the T-shaped movable seat (406) is uniformly connected with a plurality of sliding seats (411); The correcting piece further comprises a central positioning column (415), offset sensing pieces and passive driving pieces, wherein the number of the offset sensing pieces and the number of the passive driving pieces are equal to that of the sliding seats (411), each sliding seat (411) is provided with an offset sensing piece, and each magnetic attraction resetting device is provided with a passive driving piece; the bottom of the sliding seat (411) is provided with a bottom containing cavity, the bottom end of the central positioning column (415) is connected to the bottom wall of the inner cavity of the middle box seat (401), and the top end extends into the bottom containing cavity; The offset sensing part comprises a plurality of damping telescopic mechanisms which are arranged on the sliding seat (411) in a circular array; The damping telescopic mechanism comprises a converging chamber (418) arranged in the sliding seat (411), a plurality of connecting channels and a horizontal sliding chamber; The number of the connecting channels is equal to that of the horizontal sliding cavities, and the horizontal sliding cavities are distributed in the sliding seat (411) in a circular array; a reciprocating plug seat (417) is hermetically and slidably arranged in each horizontal cavity, one end of the reciprocating plug seat (417) close to the bottom containing cavity is connected with a retracting rod (416), and one end of the retracting rod (416) far away from the reciprocating plug seat (417) passes through the end of the horizontal cavity and extends into the bottom containing cavity; one side of each horizontal sliding cavity is provided with a connecting channel, one end of each connecting channel is communicated with one end of the horizontal sliding cavity far away from the bottom containing cavity, and the other end of each connecting channel is communicated with the converging cavity (418).
  2. 2. The automatic pipetting workstation as recited in claim 1, wherein the mobile positioning module (200) includes two second linear motion mechanisms (201) symmetrically arranged, and a base is commonly connected to the mover bases of the two second linear motion mechanisms (201); the top of the base is connected with a plurality of third linear motion mechanisms (202); the movable base of the third linear motion mechanism (202) is connected with a longitudinal movable frame (203), the longitudinal movable frame (203) is connected with fourth linear motion mechanisms which are longitudinally arranged, and a pipetting module (300) is detachably arranged on the movable base of each fourth linear motion mechanism.
  3. 3. The automatic pipetting station as recited in claim 2, wherein the tip module (400) further comprises a stationary gantry (403) and the first linear motion mechanism (404) is fixedly mounted in an interior cavity of the stationary gantry (403).
  4. 4. The automatic pipetting workstation as recited in claim 3, wherein the tip collection module (700) includes a support base (705); a fifth linear motion mechanism (701) is fixedly connected to the supporting seat (705); a sixth linear motion mechanism (702) is connected to the rotor seat of the fifth linear motion mechanism (701), and a suction head collecting box (703) is connected to the base of the sixth linear motion mechanism (702); an overhead tidying seat (704) positioned above the suction head collecting box (703) is connected to the rotor seat of the sixth linear motion mechanism (702), and a limiting straight groove is formed in the overhead tidying seat (704).
  5. 5. The automatic pipetting workstation as recited in claim 4, further comprising a structural frame (800); the second linear motion mechanism (201) is fixedly arranged at the top of the structural frame (800); the supporting seat (705), the linear conveying module (600), the raw material platform (500) and the fixed groove frame (403) are fixedly arranged on the bottom wall of the inner cavity of the structural frame (800).
  6. 6. The automatic pipetting workstation as recited in claim 1, wherein the follow-up pipetting station (402) is located above the center cartridge (401).
  7. 7. The automatic pipetting workstation according to claim 1, wherein the bottom end of the T-shaped movable seat (406) is positioned in the inner cavity of the middle box seat (401), and the top end of the T-shaped movable seat passes through the top opening of the middle box seat (401) and is connected to the bottom of the follow-up table (402); a smooth bottom table (410) connected to the bottom wall of the cavity of the middle box seat (401) is arranged below the sliding seat (411); the bottom of the sliding seat (411) is uniformly connected with a plurality of drag reduction rolling balls (412) which are distributed in a circular array and are attached to the smooth bottom table (410) in a rolling way.
  8. 8. The automatic pipetting workstation as recited in claim 7, wherein the number of magnetic attraction resetter is equal to the number of sliding seats (411), and one magnetic attraction resetter is arranged beside each sliding seat (411); The magnetic attraction resetter comprises a magnetic attraction block (408) and a metal block (409) which are arranged up and down; The magnetic attraction magnet (408) is connected to the bottom of the T-shaped movable seat (406), and the metal block (409) is connected to the bottom wall of the inner cavity of the middle box seat (401).
  9. 9. The automatic pipetting workstation as recited in claim 8, wherein the passive actuator comprises a guide flow tube (414), an upright cavity and a diversion channel, wherein the upright cavity and the diversion channel are arranged in the T-shaped movable seat (406), the tail section of the diversion channel is a vertical section, the bottom end of the vertical section is communicated with the upright cavity, and the insides of the horizontal sliding cavity, the connecting channel, the converging cavity (418), the guide flow tube (414) and the diversion channel are filled with liquid medium parts; One end of the diversion channel far away from the vertical section penetrates through the bottom of the T-shaped movable seat (406), and one end of the diversion flow pipe (414) is fixedly communicated with a water outlet; a lifting table (413) is arranged in the vertical cavity in a sliding way, and the top of the lifting table (413) is connected with a liquid seal rod (407) which is inserted into the vertical section of the diversion channel in a sealing way; One end of the guide flow pipe (414) extends into the sliding seat (411) and is communicated with the converging chamber (418), the other end of the guide flow pipe is fixedly connected to the bottom of the T-shaped movable seat (406), and the water outlet is inserted into an end opening of the guide channel in a sealing way; the magnetic block (408) in the magnetic attraction resetter is connected to one end of the lifting table (413) far away from the liquid seal rod (407), and one end of the magnetic block (408) far away from the lifting table (413) penetrates through the bottom of the T-shaped movable seat (406); One end of the lifting table (413) far away from the liquid seal rod (407) is also connected with a return spring, and one end of the return spring far away from the lifting table (413) is connected with the bottom wall of the vertical cavity; When the damping telescopic mechanism is in an initial state, one end of the reciprocating plug seat (417) close to the retracting rod (416) is abutted against the end part of the horizontal sliding cavity.

Description

Automatic pipetting workstation Technical Field The invention relates to the technical field of pipetting equipment, in particular to an automatic pipetting workstation. Background An automatic pipetting workstation is an automation device for biochemical laboratories, and aims to replace the traditional manual pipetting operation and realize the automation and standardization of liquid treatment. The equipment usually completes key experimental operations such as sample distribution, liquid transfer, reagent addition and the like through a combination of a mechanical arm, a precise pump system and control software. In the life science fields of drug development, clinical diagnosis, genomics, proteomics and the like, an automatic pipetting workstation has become an important tool for improving experimental efficiency and ensuring result reliability. The pipetting workstation comprises the following components: 1. rectangular coordinate three-dimensional motion system Most automatic pipetting workstations adopt an X-Y-Z three-dimensional rectangular coordinate system, are driven by a stepping motor or a servo motor, and are matched with a synchronous belt or a screw rod transmission mechanism to realize accurate positioning; 2. electric pipette One or more pipette guns or multichannel pipette guns are arranged at the tail end of the X-Y-Z moving mechanism to realize automatic liquid level detection, liquid suction and waste liquid discharge, and meanwhile, the moving mechanism is matched to realize automatic suction head acquisition and automatic suction head discarding, and the suction head is disposable, so that cross contamination among liquids is prevented; 3. related matched module The pipetting platform surface needs relative positioning/functional modules, such as a suction head box fixing module, a raw material bottle/liquid storage tank module, a centrifuge tube/porous plate module, a waste liquid collecting tank module, a waste suction head collecting module and the like, and each material has a designated position, so that the workstation can finish liquid suction and liquid discharge operations at the fixed positions. The existing pipetting workstations are mainly of the following two types: 1. the parallel 12-channel/96-channel pipetting workstation has the advantages of high flux, high efficiency and capability of simultaneously completing the preparation of multiple tubes of liquid at one time The disadvantage is that only one solution can be prepared, and customization of a single-tube solution can not be realized; 2. the single-channel pipetting workstation has the advantages that the customized preparation of each tube of solution can be realized The method has the disadvantages of low efficiency and too slow preparation speed, and cannot meet the industrial requirements. In view of this, we propose an automatic pipetting station. Disclosure of Invention The invention aims to provide an automatic pipetting workstation which solves the technical problems in the background technology. The technical scheme of the invention provides an automatic pipetting workstation which comprises a plurality of single pipetting modules arranged side by side, wherein each single pipetting module comprises a suction head module, a raw material platform, a linear conveying module, a suction head collecting module, a mobile positioning module and pipetting modules arranged on the mobile positioning module; The suction head module comprises first linear motion mechanisms with the same number as the pipetting modules, and each first linear motion mechanism is provided with a universal base table; The universal base table comprises a base part, and a following moving table is arranged above the base part; The base part comprises a correcting piece which is arranged in the inner cavity of the base part and is connected with the following moving table, and the correcting piece comprises a universal rolling device which can enable the following moving table to roll and translate and a magnetic resetting device which can perform magnetic resetting treatment on the translated following moving table. As an alternative of the technical scheme of the invention, the mobile positioning module comprises two second linear motion mechanisms which are symmetrically arranged, and a base is commonly connected with a rotor seat of the two second linear motion mechanisms; The top of the base is connected with a plurality of third linear motion mechanisms; The action seat of the third linear motion mechanism is connected with a longitudinal moving frame, the longitudinal moving frame is connected with fourth linear motion mechanisms which are longitudinally arranged, and the movable element seat of each fourth linear motion mechanism is detachably provided with a pipetting module. As an alternative to the technical scheme of the invention, the suction head module further comprises a fixed slot frame, and the first linear movement mec