CN-121824769-B - Heavy chain and light chain variable regions of bisphenol A monoclonal antibody and application thereof

CN121824769BCN 121824769 BCN121824769 BCN 121824769BCN-121824769-B

Abstract

The invention discloses a bisphenol A monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibodies contain heavy and light chain variable regions, each consisting of complementarity determining regions and framework regions, each consisting of CDR1, CDR2, and CDR 3. The monoclonal antibody can be used for preparing bisphenol A detection products. The bisphenol A colloidal gold detection test strip provided by the invention has the characteristics of high sensitivity, good specificity and strong stability.

Inventors

LI DIAN
SONG SHIYAN
GUO XIUFENG
MA MENGZHEN
ZHANG YANHAN

Assignees

北京纳百生物科技有限公司

Dates

Publication Date: 20260508
Application Date: 20260306

Claims (4)

1. A bisphenol A monoclonal antibody is characterized in that the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; the amino acid sequence of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 1; the amino acid sequence of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 2; The heavy and light chain variable regions are each comprised of a complementarity determining region and a framework region, the complementarity determining regions being comprised of CDR1, CDR2 and CDR 3; The amino acid sequence of CDR1 of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 5; The amino acid sequence of CDR2 of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 6; the amino acid sequence of CDR3 of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 7; the amino acid sequence of CDR1 of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 8; The amino acid sequence of CDR2 of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 9; The amino acid sequence of CDR3 of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 10.
2. The bisphenol a monoclonal antibody of claim 1, wherein: The gene sequence of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 3; The gene sequence of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 4.
3. Use of the bisphenol a monoclonal antibody according to claim 1 for the preparation of bisphenol a detection preparations.
4. A bisphenol A detection product is characterized by comprising a bisphenol A detection test strip and a microporous reagent, wherein the test strip is a colloidal gold detection test strip, and the microporous reagent contains the bisphenol A monoclonal antibody of claim 1 or 2.

Description

Heavy chain and light chain variable regions of bisphenol A monoclonal antibody and application thereof Technical Field The invention relates to the field of biotechnology, in particular to a heavy chain and light chain variable region of a bisphenol A monoclonal antibody and application thereof. Background Bisphenol A is a widely used industrial chemical with a chemical formula of C 15H16O2. Its most important application is as a raw material for producing polycarbonate plastics and epoxy resins. The main route of bisphenol a entry into the human body is dietary intake, e.g. migration from plastic containers or inner wall coatings of food cans into food. Bisphenol A is the major health risk that it is an endocrine disrupter, which mimics estrogen and disrupts the normal hormonal function of the human body. Animal experiments have shown that it may impair fertility, affect fetal and pediatric neurological and gonadal development, and is associated with precocious puberty, which has been classified by the European Union as a substance with reproductive toxicity. Studies suggest that it may be associated with obesity, diabetes, cardiovascular disease, and immune system dysfunction. Therefore, it is imperative to establish a safe and efficient bisphenol A biological detection method. The immunoassay method has the advantages of low cost, high efficiency, high sensitivity, low relative requirements on technicians and the like, and is suitable for rapid detection of a large number of samples. The invention aims to provide a monoclonal antibody with extremely high affinity and specificity to bisphenol A, which lays a foundation for establishing a colloidal gold test strip detection method and forming the research, development and popularization of a colloidal gold test strip product. Disclosure of Invention For this purpose, the invention provides a bisphenol A monoclonal antibody heavy chain and light chain variable region and application thereof. In order to achieve the above object, the embodiment of the present invention provides the following technical solutions: In a first aspect, the invention provides a bisphenol A monoclonal antibody, characterized in that the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; the amino acid sequence of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 1; the amino acid sequence of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 2; The heavy and light chain variable regions are each comprised of a complementarity determining region and a framework region, the complementarity determining regions being comprised of CDR1, CDR2 and CDR 3; The amino acid sequence of CDR1 of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 5; The amino acid sequence of CDR2 of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 6; the amino acid sequence of CDR3 of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 7; the amino acid sequence of CDR1 of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 8; The amino acid sequence of CDR2 of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 9; The amino acid sequence of CDR3 of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 10. In a second aspect, the present invention provides the above-described bisphenol a monoclonal antibody, characterized in that: the nucleotide sequence of the heavy chain variable region of the monoclonal antibody is shown as SEQ ID No. 3; The nucleotide sequence of the light chain variable region of the monoclonal antibody is shown as SEQ ID No. 4. In a third aspect, the invention provides an application of a bisphenol A monoclonal antibody in preparing a test strip for detecting bisphenol A. Preferably, the test strip is a colloidal gold test strip, or a fluorescent microsphere test strip, or a latex microsphere test strip. The invention has the following advantages: The invention provides a bisphenol A monoclonal antibody and application thereof, and the prepared monoclonal antibody has the characteristics of strong specificity, high sensitivity, good stability and the like, and can be used as a raw material for enzyme-linked immunosorbent assay and colloidal gold immunoassay. The invention aims to provide a bisphenol A monoclonal antibody heavy chain and light chain variable region and detection application thereof, which lays a foundation for development and popularization of ELISA, colloidal gold test strips and fluorescent immune test strips. Drawings In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those skilled in the a