CN-121970567-A - Precise positioning method for key degradation nodes of aging vitality of rice seeds

Abstract

The invention discloses a precise positioning method for key degradation nodes of aging vitality of rice seeds, and belongs to the technical field of agricultural science. The method specifically comprises the following steps of (1) carrying out artificial accelerated aging treatment on rice seeds which are in the same batch and have the same activity level and have different continuous time periods, (2) soaking seeds and accelerating germination treatment, (3) carrying out indoor normal germination test, (4) measuring seed activity indexes, (5) carrying out statistical comparison, and (6) defining aging days corresponding to the first occurrence of statistically significant reduction as key aging activity degradation nodes of the rice seeds. According to the invention, by simulating natural aging conditions, the node (namely the critical point) of which the vigor of the rice seeds is in key decline in the artificial accelerated aging process can be accurately positioned. The invention quantifies the mutation inflection point of the seed vitality under the high-strength aging condition for the first time, and provides a precise and reliable technical standard for seed aging mechanism research, vitality early warning and storability evaluation.

Inventors

• Sheng guangwei
• HUO WEINA
• LI CHENGXIA

Assignees

• 广州苗博士生物技术有限公司

Dates

Publication Date

20260505

Application Date

20260302

Claims (8)

1. 1. The accurate positioning method for the key degradation node of the aging vitality of the rice seeds is characterized by comprising the following steps: (1) Carrying out continuous artificial accelerated aging treatment on rice seeds with the same activity level in the same batch for different time periods; (2) Soaking rice seeds with different ageing durations and accelerating germination; (3) Taking rice seeds with different ageing durations as an ageing treatment group, taking unaged rice seeds as an unaged control group, and respectively carrying out indoor normal germination test; (4) Determining seed vigor index of the aging treatment group and the unaged control group; (5) Comparing the seed vitality indexes of the aging treatment group and the unaged control group with statistical significance; (6) And (3) defining the seed vitality index of the aging treatment group as the aging days corresponding to the first statistically significant decrease compared with the non-aging control group as the key aging vitality decline node of the rice seeds.
2. 2. The method for precisely positioning key degradation nodes of aging vigor of rice seeds according to claim 1, wherein in the step (1), the device for artificially accelerating aging treatment is a climatic chamber, the temperature is 44.5-45.5 ℃, the relative humidity is 95-99%, and the duration at least comprises 1 day, 2 days, 3 days, 4 days and 5 days.
3. 3. The method for precisely positioning the key degradation node of the aging vitality of the rice seeds according to claim 1, wherein in the step (2), the temperature of the seed soaking and germination accelerating treatment is 25 ℃ at room temperature, and the time is 24 hours respectively.
4. 4. The method for precisely positioning the key decline node of the aging viability of rice seeds according to claim 1, wherein in the step (3), the temperature of the indoor normal germination test is constant temperature of 25 ℃, and water and light are supplemented during the indoor normal germination test.
5. 5. The method for precisely locating key nodes of aging viability of rice seeds according to claim 1, wherein in the step (4), the measured time is 5 th day and 11 th day of indoor normal germination test.
6. 6. The method for precisely locating key nodes of deterioration of aging viability of rice seeds according to claim 1, wherein in the step (4), the seed viability index comprises germination vigor, germination rate and fresh weight of the overground parts of the seedlings.
7. 7. The method for precisely locating key nodes of aging viability of rice seeds according to claim 1, wherein in the step (5), the statistical comparison is performed by statistical significance level analysis software (P < 0.05).
8. 8. The method for precisely locating key nodes of deterioration of aging viability of rice seeds according to claim 1, wherein in step (6), the standard of significant decrease is P <0.05.

Description

Precise positioning method for key degradation nodes of aging vitality of rice seeds Technical Field The invention relates to the technical field of agricultural science, in particular to a precise positioning method for key degradation nodes of aging vitality of rice seeds. Background In the storage and circulation links of rice seeds, the rice seeds are easily affected by high-temperature and high-humidity environments to cause aging, so that the activity of the seeds is reduced, and the field emergence rate and the final yield are directly affected. Therefore, the seed aging degree is accurately estimated, early warning is carried out on the aging process, and the method has important significance in guaranteeing agricultural production and reducing economic loss. Currently, an artificial accelerated aging test is a common method for rapidly evaluating the storage endurance and aging degree of rice seeds. However, the conventional methods have focused on determining the final vigor (e.g., germination rate) of rice seeds after a single, fixed, long-term aging treatment. This "endpoint" assessment does not reveal that aging is a dynamic process and does not accurately identify the critical turning point at which the vigor of the rice seed drops sharply from stable maintenance to onset. Because of the lack of accurate definition and determination methods for the key node, quantitative analysis and effective intervention are difficult to perform in the fields of physiological research, vigor monitoring and early warning, germplasm resource evaluation and the like of rice seeds. Therefore, how to develop a method capable of precisely and quantitatively locating the initial key decline node in the aging process of rice seeds is a urgent problem to be solved by those skilled in the art. Disclosure of Invention Therefore, the invention aims to provide a precise positioning method for key degradation nodes of aging vitality of rice seeds, so as to solve the defects in the prior art. In order to achieve the above purpose, the present invention adopts the following technical scheme: A precise positioning method for key degradation nodes of aging vitality of rice seeds specifically comprises the following steps: (1) Carrying out artificial accelerated aging treatment on rice seeds (bare seeds) which are in the same batch and have the same activity level and are continuously of different durations; (2) Soaking rice seeds with different ageing durations and accelerating germination; (3) Taking rice seeds with different ageing durations as an ageing treatment group, taking unaged rice seeds (bare seeds) as an unaged control group, and respectively carrying out indoor normal germination test; (4) Determining seed vigor index of the aging treatment group and the unaged control group; (5) Comparing the seed vitality indexes of the aging treatment group and the unaged control group with statistical significance; (6) And (3) defining the seed vitality index of the aging treatment group as the aging days corresponding to the first statistically significant decrease compared with the non-aging control group as the key aging vitality decline node of the rice seeds. Further, in the step (1), the device for artificially accelerating the aging treatment is a climatic chamber, the temperature is 44.5-45.5 ℃, preferably 45 ℃, the Relative Humidity (RH) is 95-99%, preferably 97%, and the time period at least comprises 1 day, 2 days, 3 days, 4 days and 5 days. Further, in the step (2), the temperatures of seed soaking and germination accelerating treatment are 25 ℃ at room temperature, and the time is 24 hours. Further, in the step (3), the temperature of the indoor normal germination test is constant at 25 ℃, and the water and the light are supplemented during the period. Further, in the above step (4), the measured time is 5 th and 11 th days of the indoor normal germination test. Further, in the step (4), the seed vigor index includes germination vigor, germination rate and fresh weight of the aerial parts of the seedlings. Further, in the step (5), the statistical comparison is performed by using statistical significance level analysis software (P < 0.05). Further, in the above step (6), the standard of significant drop is P <0.05. Compared with the prior art, the invention has the following beneficial effects: 1. Key mutation points in the aging process are quantized for the first time According to the invention, through a precisely controlled time sequence experiment, it is disclosed for the first time that under the specific high-strength aging condition of 45 ℃/97%RH, the vigor of rice seeds does not drop at a constant speed, but the 'cliff' drop from the quantity to the quality change occurs on a specific day (such as the 3 rd day), and the node is precisely defined as the 'key degradation node of the aging vigor'. 2. Provides accurate early warning tool The invention can send out early warning at the starting point of irrev