CN-121970586-A - Application of nano zinc oxide in improving biomass and active ingredient content of liquorice

Abstract

The invention discloses application of nano zinc oxide in improving biomass and active ingredient content of liquorice, and relates to the technical field of plant cultivation, so that the biomass and active ingredient content of the liquorice are improved. The plant height, root length and dry matter accumulation of the liquorice can be obviously improved by externally applying the nano zinc oxide, the biomass of the liquorice is increased, the synthesis and accumulation of main active ingredients such as glycyrrhizic acid, glycyrrhizin and the like are effectively promoted, the physiological state of the liquorice under stress conditions such as salt stress can be improved, the stress resistance is enhanced, the in-vivo ion balance and the oxidation-reduction steady state are regulated, and the synergistic improvement of the growth and quality of the liquorice is realized.

Inventors

• SUN ZHIRONG
• Xu Gonghao
• LIANG SHUANG
• WANG SHANGTAO

Assignees

• 北京中医药大学

Dates

Publication Date

20260505

Application Date

20260213

Claims (10)

1. 1. An application of nanometer zinc oxide in increasing biomass and active ingredient content of Glycyrrhrizae radix is provided.
2. 2. The use according to claim 1, characterized in that the particle size of the nano zinc oxide is 1-50nm.
3. 3. Use according to claim 1 or 2, characterized in that the nano zinc oxide is added as zinc source in water or nutrient solution, and licorice in the growing period is irrigated and/or irrigated.
4. 4. The method according to claim 3, wherein the effective concentration of nano zinc oxide in the water or nutrient solution is 5-60mg/L; the times of irrigation are 3-6 times, and the times of sprinkling irrigation are 2-6 times.
5. 5. The use according to claim 3 or 4, wherein the effective amount of nano zinc oxide is (0.5-5) g/m 2 , calculated as the land area of licorice.
6. 6. The use according to claim 1 or 2, wherein said increasing the biomass of licorice comprises: the plant height and root length of the liquorice are improved; increasing the dry weight of the aerial part and the dry weight of the underground part of the liquorice; Improving root-cap ratio of Glycyrrhrizae radix.
7. 7. Use according to claim 1 or 2, characterized in that the active ingredient comprises glycyrrhizin and/or glycyrrhizic acid.
8. 8. The use according to claim 1 or 2, wherein the concentration of total flavonoids is 40mg/g or more based on the dry weight of the roots of licorice, and/or, The concentration of glycyrrhizin is 2.5mg/g or more and the concentration of glycyrrhizin is 0.7mg/g or more based on the dry weight of the underground portion of licorice.
9. 9. The use according to claim 1 or 2, wherein the root-cap ratio of licorice is 0.55 or more.
10. 10. The use according to claim 1 or 2, wherein the nano zinc oxide is used for increasing biomass and active ingredient content of licorice under salt stress conditions, wherein the salt stress conditions comprise sodium chloride concentration of 130-180mmol/L in the water or nutrient solution containing the nano zinc oxide.

Description

Application of nano zinc oxide in improving biomass and active ingredient content of liquorice Technical Field The invention relates to the technical field of plant cultivation, in particular to application of nano zinc oxide in improving biomass and active ingredient content of liquorice. Background Soil salinization is considered to be one of the most damaging factors in various abiotic stresses such as drought, salt and alkali, extreme temperatures, heavy metals, organic pollutants and the like, and has become a major factor limiting agricultural productivity and global grain safety. Planting salt-tolerant plant varieties with economic value in saline-alkali soil is considered as a high-efficiency practical strategy for sustainable utilization of saline-alkali soil resources. Licorice (Glycyrrhiza uralensis Fischer.) is a perennial leguminous plant and has extremely high commercial value. The root and rhizome are rich in triterpene saponin, flavonoid and other bioactive components, and have various pharmacological activities such as immunoregulation and antiviral. Licorice is widely used as a traditional medicinal material in the east-west countries, and has a great deal of application in the fields of foods, light industry and cosmetics. With the promotion of global health awareness and the increase of dependence on natural medicines, the demand of liquorice is rapidly increased, so that wild resources are exhausted, and artificial cultivation becomes a main supply mode. Therefore, there is a need to provide a method for increasing the biomass and active ingredient content of licorice. Disclosure of Invention The invention aims to provide an application of nano zinc oxide in improving biomass and active ingredient content of liquorice, thereby improving the biomass and active ingredient content of the liquorice. In order to achieve the above objective, the first aspect of the present invention provides an application of nano zinc oxide in increasing biomass and active ingredient content of licorice. Compared with the prior art, the invention discovers a new application of nano zinc oxide, and can improve the biomass and the active ingredient content of liquorice. The plant height, root length and dry matter accumulation of the liquorice can be obviously improved by externally applying the nano zinc oxide, the biomass of the liquorice is increased, the synthesis and accumulation of main active ingredients such as glycyrrhizic acid, glycyrrhizin and the like are effectively promoted, the physiological state of the liquorice under stress conditions such as salt stress can be improved, the stress resistance is enhanced, the in-vivo ion balance and the oxidation-reduction steady state are regulated, and the synergistic improvement of the growth and quality of the liquorice is realized. Further, the particle size of the nano zinc oxide is 1-50nm. In the invention, when the particle size of the nano zinc oxide meets the above range, the biomass and the active ingredient content of the liquorice can be further improved. Further, the nano zinc oxide is used as a zinc source to be added into water or nutrient solution, and licorice in a growing period is irrigated and/or irrigated. According to the invention, the zinc fertilizer is applied by adopting the method, so that the method is simple and convenient to operate, low in application amount, environment-friendly, capable of reducing the application amount of the traditional zinc fertilizer, avoiding the zinc element waste and the environment risk, and suitable for popularization and application in standardized cultivation of medicinal liquorice. Further, the effective concentration of the nano zinc oxide in the water or the nutrient solution is 5-60mg/L. In the invention, when the effective concentration of nano zinc oxide in water or nutrient solution meets the above range, the biomass and the active ingredient content of the liquorice can be further improved. Further, the number of times of irrigation is 3-6, and the number of times of sprinkling irrigation is 2-6. Further, the effective dosage of the nano zinc oxide is (0.5-5) g/m 2 based on the land area of the liquorice. In the invention, when irrigation or sprinkling conditions or the effective amount of nano zinc oxide satisfies the above range, the biomass and the active ingredient content of licorice can be further improved. Further, the increasing biomass of licorice comprises: the plant height and root length of the liquorice are improved; increasing the dry weight of the aerial part and the dry weight of the underground part of the liquorice; Improving root-cap ratio of Glycyrrhrizae radix. Further, the active ingredient includes glycyrrhizic acid and/or glycyrrhizin. Further, the concentration of total flavonoids is 40mg/g or more based on the dry weight of the roots of licorice. Further, the concentration of glycyrrhizin is 2.5mg/g or more and the concentration of glycyrrhizin is 0.7mg/g or more