Search

CN-121970647-A - Cultivation method of mycorrhizal edible fungus symbiotic seedlings

CN121970647ACN 121970647 ACN121970647 ACN 121970647ACN-121970647-A

Abstract

The invention discloses a cultivation method of mycorrhizal edible fungi symbiotic seedlings, which relates to the technical field of edible fungi cultivation and specifically comprises the following steps of S1, cultivating symbiotic plant seedlings; the method comprises the steps of S21 mycelium culture, S22 purification culture, inoculation of mycelium obtained in the step S21 into a new solid culture medium, standing culture at 24-26 ℃ until the mycelium grows, S23 fermentation culture, inoculation of mycelium obtained in the step S22 onto a liquid fermentation culture medium, culture on a 25 ℃ constant temperature shaking table to obtain liquid strains, S3 infection, injection of the liquid strains obtained in the step S23 into a symbiotic seedling compound culture medium obtained in the step S13, culture in an illumination incubator, periodic nutrient solution supplement during culture, and formation of the symbiotic seedlings of the mycorrhizal edible fungi after culture for 60-90 days. The method has high mycorrhiza infection rate and symbiotic seedling survival rate, and is suitable for popularization.

Inventors

  • HAO LONGFEI
  • LIU TINGYAN
  • YUE YONGJIE
  • BAI SHULAN

Assignees

  • 内蒙古农业大学

Dates

Publication Date
20260505
Application Date
20231024

Claims (8)

  1. 1. A cultivation method of mycorrhizal edible fungus symbiotic seedlings is characterized by comprising the following steps: S1, culturing symbiotic plant seedlings: s11, sterilizing, namely selecting healthy full symbiotic plant seeds without plant diseases and insect pests, soaking the healthy full symbiotic plant seeds into 1% potassium permanganate solution for 1-2 hours to perform surface sterilization, taking out, and flushing with sterile water; s12, accelerating germination, namely immersing the symbiotic plant seeds in the step S11 into the accelerating germination liquid for 2-3h; S13, culturing, namely sowing the symbiotic plant seeds in the step S12 into a symbiotic seedling composite culture medium, and periodically adding nutrient solution into the culture medium until 2-3 roots grow; s2, culturing edible fungus mycelia with mycorrhizae: S21, mycelium culture, namely collecting healthy and healthy wild mycorrhizal edible fungi without diseases, taking fungus meat tissues, inoculating the fungus meat tissues on a solid culture medium, and culturing at 24-26 ℃ until the mycelia grow fully; S22, purifying and culturing, namely picking hypha in the step S21, inoculating the hypha into a new solid culture medium, and standing and culturing at 24-26 ℃ until the hypha grows fully; s23, fermenting and culturing, namely inoculating hypha in the step S22 on a liquid fermentation culture medium, and culturing on a 25 ℃ constant-temperature shaking table to obtain liquid strain; and S3, infection, namely injecting the liquid strain in the step S23 into the symbiotic seedling composite culture medium in the step S13, placing the culture medium in an illumination incubator for culture, periodically supplementing nutrient solution during the culture period, and culturing for 60-90 days to form the mycorrhizal edible fungus symbiotic seedling.
  2. 2. The method for cultivating mycorrhizal edible fungus symbiotic seedlings according to claim 1, wherein in the step S12, the germination accelerating liquid comprises the following raw materials in parts by weight of 0.05g of 1% calcium chloride, 0.1g of 2% copper sulfate, 0.2g of gibberellin, 2g of chitosan and 1L of water, and the sterilization is carried out for 3 hours at the temperature of 121 ℃.
  3. 3. The method for cultivating mycorrhizal edible fungus symbiotic seedlings according to claim 1, wherein in the step S13, the nutrient solution comprises the following raw materials in parts by weight of 2g of urea, 1g of monopotassium phosphate, 0.5g of glucose, 0.5g of algal polysaccharide, 0.01g of ferric citrate, 0.2g of magnesium sulfate, 0.01g of zinc sulfate, 0.02g of ferric chloride, 0.01g of copper sulfate and 1L of water.
  4. 4. The method for cultivating symbiotic seedlings of mycorrhizal edible fungi according to claim 1, wherein in the step S13, the formula of the symbiotic seedling compound culture medium comprises 1g of vermiculite, 1.2g of medical stone, 2g of humus soil, 1.5g of corn meal, 5g of traditional Chinese medicine residues, 2g of feather meal, 5g of peptone, 2g of yeast extract powder, 2g of glucose, 2g,K2HPO4 1g,MgSO4-7H2O 0.05g,CaCl2 0.01g,NaCl 0.01g of chitosan, 100mL of distilled water and sterilizing at the pH=5-6,121 ℃ for 3 hours.
  5. 5. The method for cultivating mycorrhizal edible fungus symbiotic seedlings according to claim 1, wherein in the step S21, the formula of the solid culture medium comprises 5g of peptone, 2g of yeast extract powder, 20g of glucose, 10g,K2HPO4 1g,MgSO4. 7H2O 0.5g,CaCl2 0.05g,NaCl 0.1g of chitosan, 20g of agar and 1L of distilled water, and the pH=5-6,121 ℃ is sterilized for 3 hours.
  6. 6. The method for cultivating mycorrhizal edible fungus symbiotic seedlings according to claim 1, wherein in the step S23, the liquid fermentation medium comprises 5.0g of peptone, 2.0g of yeast extract powder, 20.0g of glucose, 10g,K2HPO4 1.0g,MgSO4. 7H2O 0.5g,CaCl2 0.05g,NaCl0.1g of chitosan, 100mL of Chinese medicinal extract, 900mL of distilled water and sterilization at pH=5-6,121 ℃ for 3 hours.
  7. 7. The cultivation method of the mycorrhizal edible fungus symbiotic seedlings is characterized in that the raw material components and the weight portions of the traditional Chinese medicine residues are 10 parts of gynostemma pentaphylla, 6 parts of honey-fried licorice root, 8 parts of ligusticum wallichii, 4 parts of pumpkin seed and 4 parts of jujube, the preparation method of the traditional Chinese medicine residues comprises the steps of taking gynostemma pentaphylla, honey-fried licorice root, ligusticum wallichii, pumpkin seed and jujube according to the weight portions, adding purified water which is 6 times of the weight portions, soaking for 2 hours, boiling for 2 hours, filtering to obtain an extracting solution, repeatedly extracting for 3 times, combining the extracting solutions obtained in 3 times, and respectively obtaining the traditional Chinese medicine residues and the traditional Chinese medicine extracting solution, and sterilizing for 3 hours at 121 ℃ for later use.
  8. 8. The method for cultivating mycorrhizal edible fungi symbiotic seedlings according to claim 6, wherein the traditional Chinese medicine extracting solution is the traditional Chinese medicine extracting solution according to claim 7.

Description

Cultivation method of mycorrhizal edible fungus symbiotic seedlings Technical Field The invention relates to the technical field of edible fungus cultivation, in particular to a cultivation method of mycorrhizal edible fungus symbiotic seedlings. Background Some of the edible fungi are symbiotic with the roots of plants, and mycelia are wound on the root surfaces or penetrate into the roots. The symbiotic plants supply the carbohydrates synthesized by photosynthesis to the living of the bacteria, and the water and inorganic salts absorbed by the mycelium from the soil, the secreted vitamins, auxins and the like are supplied to the plants for utilization, so that the mutual utilization close relationship is formed, and the edible fungi are called mycorrhizal edible fungi. The edible fungi form mycorrhizae, which is an ecological relationship formed in long-term natural environment. The relationship is easily damaged or changed, and the life of plants or edible fungi is adversely affected or even not normally living. Therefore, the mycorrhizal infection rate and the survival rate of the symbiotic seedlings are low in the existing artificial cultivation symbiotic plants-mycorrhizal edible fungi symbiotic seedlings, and great difficulty exists in the development of artificial cultivation. Based on the method, a cultivation method of mycorrhizal edible fungus symbiotic seedlings with high mycorrhizal infection rate and high symbiotic seedling survival rate is developed, and the method is a technical problem to be solved by the people in the field. Disclosure of Invention Aiming at the problems, the invention provides a cultivation method of mycorrhizal edible fungus symbiotic seedlings, which solves the problems of lower mycorrhizal infection rate and lower survival rate of the symbiotic seedlings of artificially cultivated symbiotic plants-mycorrhizal edible fungus symbiotic seedlings in the prior art. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: The invention provides a cultivation method of mycorrhizal edible fungus symbiotic seedlings, which specifically comprises the following steps: S1, culturing symbiotic plant seedlings: s11, sterilizing, namely selecting healthy full symbiotic plant seeds without plant diseases and insect pests, soaking the healthy full symbiotic plant seeds into 1% potassium permanganate solution for 1-2 hours to perform surface sterilization, taking out, and flushing with sterile water; s12, accelerating germination, namely immersing the symbiotic plant seeds in the step S11 into the accelerating germination liquid for 2-3h; S13, culturing, namely sowing the symbiotic plant seeds in the step S12 into a symbiotic seedling composite culture medium, and periodically adding nutrient solution into the culture medium until 2-3 roots grow; s2, culturing edible fungus mycelia with mycorrhizae: S21, mycelium culture, namely collecting healthy and healthy wild mycorrhizal edible fungi without diseases, taking fungus meat tissues, inoculating the fungus meat tissues on a solid culture medium, and culturing at 24-26 ℃ until the mycelia grow fully; S22, purifying and culturing, namely picking hypha in the step S21, inoculating the hypha into a new solid culture medium, and standing and culturing at 24-26 ℃ until the hypha grows fully; s23, fermenting and culturing, namely inoculating hypha in the step S22 on a liquid fermentation culture medium, and culturing on a 25 ℃ constant-temperature shaking table to obtain liquid strain; and S3, infection, namely injecting the liquid strain in the step S23 into the symbiotic seedling composite culture medium in the step S13, placing the culture medium in an illumination incubator for culture, periodically supplementing nutrient solution during the culture period, and culturing for 60-90 days to form the mycorrhizal edible fungus symbiotic seedling. Further, in the step S12, the germination accelerating liquid comprises the following raw materials in parts by weight of 0.05g of 1% calcium chloride, 0.1g of 2% copper sulfate, 0.2g of gibberellin, 2g of chitosan, 1L of water and sterilization at 121 ℃ for 3 hours. Further, in the step S13, the nutrient solution comprises the following raw materials in parts by weight of 2g of urea, 1g of monopotassium phosphate, 0.5g of glucose, 0.5g of algal polysaccharide, 0.01g of ferric citrate, 0.2g of magnesium sulfate, 0.01g of zinc sulfate, 0.02g of ferric chloride, 0.01g of copper sulfate and 1L of water. Further, in the step S13, the symbiotic seedling composite culture medium comprises 1g of vermiculite, 1.2g of medical stone, 2g of humus soil, 1.5g of corn meal, 5g of traditional Chinese medicine residues, 2g of feather meal, 5g of peptone, 2g of yeast extract powder, 2g of glucose, 2g of chitosan, 0.01g of K 2HPO4 1g,MgSO4·7H2O 0.05g,CaCl2, 0.01g of NaCl, 100mL of distilled water and sterilizing for 3 hours at the pH of between 5 and 6,121 ℃. Further, in the s