CN-121970654-A - Method for rapidly identifying salt tolerance of oat variety resources in large scale and application

CN121970654ACN 121970654 ACN121970654 ACN 121970654ACN-121970654-A

Abstract

The invention belongs to the technical field of crop salt tolerance identification, and particularly relates to a method for rapidly identifying salt tolerance of oat variety resources in a large scale and application thereof. The invention provides a method for rapidly identifying the salt tolerance of oat variety resources in a large scale, which combines different development periods, is based on multi-index measurement analysis of morphology and physiological change, and realizes quantitative sorting and grading of variety salt tolerance by using principal component analysis dimension reduction and membership function comprehensive evaluation. The method has the characteristics of short period, controllable environment, rapidness, high efficiency and the like, and can provide a technical method for developing the research on the cultivation of salt tolerance varieties of oat and similar plants and the salt tolerance mechanism.

Inventors

LI JIANJIAN
ZONG JUNQIN
XU XIWEN
WANG HAORAN
LI XIAOHUI
WANG HUAN
ZHANG YINGHUI
LI LING
LU HAILONG
HAO DONGLI

Assignees

江苏省中国科学院植物研究所

Dates

Publication Date: 20260505
Application Date: 20260319

Claims (10)

1. A method for rapidly identifying salt tolerance of oat variety resources in large scale, which is characterized by comprising the following steps: Salt stress treatment is carried out in the germination period of the oat, and the germination characteristics of the oat seeds after the salt stress treatment are measured, wherein the germination characteristics of the oat seeds comprise relative germination rate, relative germ length and relative radicle length; Evaluating the salt tolerance of the oat seeds in the germination period according to the germination characteristics of the oat seeds to obtain a comprehensive evaluation value of the salt tolerance of the oat in the germination period; Performing salt stress treatment in the growing period of the oat seedling, and measuring the growth characteristics of the oat seedling after the salt stress treatment, wherein the growth characteristics of the oat seedling comprise relative plant height, relative root length, relative root number and relative SPAD value; According to the growth characteristics of the oat seedlings, salt tolerance evaluation in the seedling growth period is carried out, and comprehensive evaluation values of the salt tolerance in the oat seedling growth period are obtained; The comprehensive evaluation value of the salt tolerance of the oat in the germination period and the comprehensive evaluation value of the salt tolerance of the oat in the seedling growth period are subjected to weighted analysis to obtain comprehensive weighted values of the salt tolerance evaluation; The salt tolerance evaluation is carried out by adopting a principal component analysis method and a membership function analysis method.
2. The method of claim 1, wherein the salt stress treatment employs a salt concentration gradient treatment.
3. The method according to claim 2, wherein the salt stress treatment sets 1 salt concentration gradient when the salt stress treatment is performed during the germination period of the oat, and the salt concentration is 80-150 mM.
4. The method according to claim 2, wherein the salt stress treatment sets 3 salt concentration gradients during the salt stress treatment in the growing period of oat seedlings, and the 3 salt concentrations are 80-120 mM, 130-170 mM and 180-220 mM.
5. The method of claim 1, wherein the oat seedlings are cultivated by hydroponic methods.
6. The method according to claim 1, wherein the growth phase of oat seedlings is a two-leaf one-heart phase.
7. A method according to claim 1 or 3, wherein the number of days of salt stress treatment is 3-7 d when salt stress treatment is performed during the germination period of oat.
8. The method according to any one of claims 1, 4 to 6, wherein the number of days of salt stress treatment is 5 to 10 d when salt stress treatment is performed in the growth period of oat seedlings.
9. The method according to claim 1, further comprising performing a cluster analysis on the salt tolerance evaluation integrated weight after obtaining the salt tolerance evaluation integrated weight.
10. Use of the method of any one of claims 1-9 for cultivating salt tolerant oat germplasm.

Description

Method for rapidly identifying salt tolerance of oat variety resources in large scale and application Technical Field The invention belongs to the technical field of crop salt tolerance identification, and particularly relates to a method for rapidly identifying salt tolerance of oat variety resources in a large scale and application thereof. Background The oat (Avena satival) is a annual grain and feed dual-purpose crop of the Avena of Gramineae, has rich nutrition of stems and leaves, good palatability, is a high-quality forage grass resource, has high nutrition and health care value due to the fact that the seeds are rich in dietary fibers and beta-glucan, has comprehensive stress resistance characteristics of drought resistance, cold resistance, barren resistance, salt and alkali resistance and the like, and is known as a pioneer crop of the saline and alkaline land. The oat has extremely high planting value in the salting area, but the salt tolerance of different varieties of materials is greatly different. Therefore, the development of the identification of the salt-tolerant germplasm and the variety breeding of the oat has important significance for the biological treatment of the saline-alkali soil, the sustainable development of the agriculture and animal husbandry and the national grain safety. Currently, crop salt tolerance identification is mostly evaluated by single development stage (such as germination stage or seedling stage), single stress treatment or few morphological indexes. The phenotypic characteristics and physiological response of plants under salt stress are easily influenced by various factors such as species characteristics, growth states and the like, and a unified and standard identification index system is not formed at present for evaluating the salt tolerance of oat. Meanwhile, the traditional field identification method has the problems of low efficiency, limited identification flux and the like, and is difficult to meet the actual requirements of rapid screening and accurate identification of large-scale oat salt-tolerant germplasm resources. Therefore, a systematic, efficient and accurate comprehensive evaluation method for oat salt tolerance is needed. Disclosure of Invention The invention aims to provide a method for rapidly and massively identifying the salt tolerance of oat variety resources and application thereof, wherein the method can realize the salt tolerance evaluation and screening of a large amount of oat variety resources in a short period, and has the advantages of simple operation, low cost and accurate identification result. The invention provides a method for rapidly identifying salt tolerance of oat variety resources in a large scale, which comprises the following steps: Salt stress treatment is carried out in the germination period of the oat, and the germination characteristics of the oat seeds after the salt stress treatment are measured, wherein the germination characteristics of the oat seeds comprise relative germination rate, relative germ length and relative radicle length; Evaluating the salt tolerance of the oat seeds in the germination period according to the germination characteristics of the oat seeds to obtain a comprehensive evaluation value of the salt tolerance of the oat in the germination period; Performing salt stress treatment in the growing period of the oat seedling, and measuring the growth characteristics of the oat seedling after the salt stress treatment, wherein the growth characteristics of the oat seedling comprise relative plant height, relative root length, relative root number and relative SPAD value; According to the growth characteristics of the oat seedlings, salt tolerance evaluation in the seedling growth period is carried out, and comprehensive evaluation values of the salt tolerance in the oat seedling growth period are obtained; The comprehensive evaluation value of the salt tolerance of the oat in the germination period and the comprehensive evaluation value of the salt tolerance of the oat in the seedling growth period are subjected to weighted analysis to obtain comprehensive weighted values of the salt tolerance evaluation; The salt tolerance evaluation is carried out by adopting a principal component analysis method and a membership function analysis method. As a preferred embodiment, the salt stress treatment employs a salt concentration gradient treatment. As a preferable scheme, when salt stress treatment is carried out in the germination period of the oat, 1 salt concentration gradient is set in the salt stress treatment, and the salt concentration is 80-150 mM. As a preferable scheme, when salt stress treatment is carried out in the growing period of oat seedlings, 3 salt concentration gradients are set in the salt stress treatment, wherein the 3 salt concentrations are 80-120 mM, 130-170 mM and 180-220 mM. As a preferred scheme, the oat seedlings are cultivated by a water culture method. As a preferred