CN-121970703-A - Method for producing all-male micropterus salmoides based on soaking-induced micropterus salmoides sexual inversion

Abstract

The application discloses a method for producing all-male larch based on soaking-induced larch sexual inversion, in particular to the field of artificial propagation of all-male larch, which comprises the following steps of S01, taking a larch fry obtained by hatching as an initial bait when the larch fry is in flat trip; S02, transferring the open largemouth black weever fries into a water body with a dissolved sex reversal induction reagent, feeding 3 times per day during the period, soaking and cultivating the fries for 60 days, and transferring the largemouth black weever fries into a conventional cultivation water body for cultivation after the soaking and cultivating for 60 days, wherein the obtained male population capable of producing semen after sexual maturity is the total male largemouth black weever fries. The method can directly obtain the sex reversal fish with high survival rate and low deformity rate by soaking the largemouth black bass seedlings in the sex reversal induction reagent with specific concentration, thereby realizing large-scale cultivation of the total male largemouth black bass.

Inventors

• HU FANGZHOU
• WEN MING
• LI WUHUI
• LIU QIZHI
• LIU SHAOJUN
• Xiong Zheduo
• ZHANG CHUN
• ZHONG HAITAO
• SUN YU
• WANG SHI
• WU CHANG
• SHEN ZHONGYUAN

Assignees

• 湖南师范大学
• 岳麓山实验室

Dates

Publication Date

20260505

Application Date

20260317

Claims (7)

1. 1. A method for producing all-male micropterus salmoides based on soaking-induced micropterus salmoides sexual inversion, which is characterized by comprising the following steps: s01, taking the hatching larch fries as the initial bait when the larch fries are parallel to the swimming course; S02, transferring the open largemouth black weever fries into a water body with a dissolved sex reversal induction reagent, feeding 3 times per day, and soaking and cultivating fries for 60 days; S03, after soaking and culturing for 60 days, transferring the largehead jewfish fries into a conventional culture water body for culturing, and obtaining a male population capable of producing semen after sexual maturity, namely the total male largehead jewfish.
2. 2. The method for producing the all-male micropterus salmoides based on soaking-induced micropterus salmoides sex reversal according to claim 1, wherein in the step S01, the micropterus salmoides Miao Ping are hatched for 5-7 days.
3. 3. The method for producing the all-male micropterus salmoides based on soaking-induced micropterus salmoides sex reversal according to claim 1, wherein in the step S02, the sex reversal inducing agent is etonogestrel with the concentration of 100-200 ng/L.
4. 4. The method for producing the all-male micropterus salmoides based on soaking-induced micropterus salmoides sex reversal according to claim 3, wherein the etoposide is prepared by fully dissolving absolute ethyl alcohol at 60-70 ℃ to prepare a mother solution with the concentration of 100 mg/L, and then adding the mother solution into a cultivation barrel in proportion to maintain the final concentration of the etoposide in a cultivation barrel water body within a range of 100-200 ng/L.
5. 5. The method according to claim 4, wherein in step S02, the cultivation density is 200 tails/m 3 and the cultivation bucket body of water is 50L.
6. 6. The method according to claim 5, wherein in step S02, artemia are fed for the first 7 days during the feeding period, 7-15 days are converted into feed powder with a protein content of >50%, and aerated tap water of 100-200ng/L of etonogestrel is used for water change once every 3 days, wherein the water change amount is one third of the volume of the water in the cultivation tanks.
7. 7. The method according to claim 1, wherein in the step (3), the verification method of the all male micropterus salmoides comprises the steps of randomly selecting 30 tail XX type common micropterus salmoides, carrying out PCR amplification on the genetic sex through a specific molecular marker, and carrying out agarose gel electrophoresis analysis and identification to obtain the genetic sex, wherein the genetic sex is obtained through gonadal HE staining identification.

Description

Method for producing all-male micropterus salmoides based on soaking-induced micropterus salmoides sexual inversion Technical Field The application relates to the field of artificial propagation of all-male micropterus salmoides, in particular to a method for producing all-male micropterus salmoides based on soaking-induced micropterus salmoides sexual inversion. Background The largemouth black bass, commonly known as the California perch, belongs to the weever orders, weever subgenera and solar fish families, is native to fresh water areas in North America, and is introduced into China for the first time in 1983. By virtue of the remarkable advantages of tender and delicious meat quality, short cultivation period, strong environmental adaptability, easiness in catching and the like, the cultivation scale is continuously enlarged, and the method has been developed into one of important famous fresh water cultivation varieties in China. Research shows that male and female individuals of the largemouth bass show obvious dichotomy in energy distribution after gonad development is started. Female individuals use a large amount of ingested energy preferentially for the development of ovaries. The physiological energy redistribution strategy directly leads to obvious slow down of the growth rate of female micropterus salmoides, and meanwhile, the bait coefficient is correspondingly increased, so that the economic benefit of large-scale cultivation is restricted. In view of this, cultivating the group of all-male largemouth black bass is the most effective way to break through the current industrial development bottleneck and improve the cultivation efficiency and economic return. The sex reversal technology can produce a parthenogenetic population, solves the problems of growth difference, propagation loss and the like caused by sex in cultivation, and can realize sex orientation control through hormone induction (such as methyl testosterone) and temperature induction at present so as to obtain the all-male micropterus salmoides. In the past hormone induction, hormone is often used for treating feed, the aim of higher sexual inversion rate is achieved by feeding the feed, but in the actual operation process, the feed for the young Japanese perch has certain operation difficulty, the feed is fed with the hormone to cause stress to the fish body so as to reduce the survival rate of offspring, and the temperature induction can successfully cultivate the sexual inversion fish with high survival rate and low deformity rate by accurately regulating and controlling the water temperature, however, in the large-scale cultivation practice, the energy consumption and equipment investment cost required for maintaining the constant water temperature for a long time are higher, and the wide application of the technology in industry is limited. In view of the above, the method for large-scale production of the all-male micropterus salmoides is designed to be more energy-saving, stable and easy to operate, and also needs to meet the requirements of technicians on high survival rate, high sex reversal rate and low deformity rate of the micropterus salmoides for inducing the sex reversal of the micropterus salmoides. Disclosure of Invention The invention provides a method for producing all-male micropterus salmoides based on soaking-induced micropterus salmoides sexual inversion. The method can directly obtain the sex reversal fish with high survival rate and low deformity rate by soaking the largemouth black bass seedlings in the sex reversal induction reagent with specific concentration, thereby realizing large-scale cultivation of the total male largemouth black bass. The aim of the invention is realized by the following technical scheme: a method for producing all-male micropterus salmoides based on soaking-induced micropterus salmoides sexual inversion, comprising the following steps: s01, taking the hatching larch fries as the initial bait when the larch fries are parallel to the swimming course; S02, transferring the open largemouth black weever fries into a water body with a dissolved sex reversal induction reagent, feeding 3 times per day, and soaking and cultivating fries for 60 days; S03, after soaking and culturing for 60 days, transferring the largehead jewfish fries into a conventional culture water body for culturing, and obtaining a male population capable of producing semen after sexual maturity, namely the total male largehead jewfish. Preferably, in the step S01, the perch Miao Ping reaches the fry to be hatched for 5 to 7 days. Preferably, in step S02, the sex reversal inducing agent is etonogestrel at a concentration of 100-200 ng/L. The preferable preparation method of the etogestrel comprises the steps of firstly fully dissolving the etogestrel in absolute ethyl alcohol at 60-70 ℃ to prepare mother solution with the concentration of 100 mg/L, and then adding the mother solution into a cultivation barrel in p