CN-121970728-A - Novel method for feeding and controlled release of biological control agents

CN121970728ACN 121970728 ACN121970728 ACN 121970728ACN-121970728-A

Abstract

The present invention relates to a novel method for feeding and controlled release of biological control agents. A feeding composition comprising a predatory mite population comprising at least one mite species of the genus phytoseiidae capable of spawning for at least 2 generations and comprising at least one predatory mite population from the sub-order DoS.and optionally from a species of the family phytoseiidae. Methods for feeding the same are also disclosed.

Inventors

TABIKH ALI
T. Katz
A. Grossman
STEINBERG STEFAN

Assignees

生物蜜蜂赛德利亚胡有限公司

Dates

Publication Date: 20260505
Application Date: 20190305
Priority Date: 20180305

Claims (15)

1. A method for rearing a predatory mite population comprising at least one mite species of the genus phytoseiid, the method comprising: a. Providing a composition comprising a predatory mite population comprising at least one mite species of the genus phytoseiidae and a predatory mite population comprising at least one individual from a valve-less sub-target mite species; b. Allowing individuals of the predatory mite population to continue predating individuals of the valveless sub-population for at least 2 generations; Wherein the valve sub-order predators are selected from the group consisting of non-viable mites, non-viable eggs, and combinations thereof.
2. The method of claim 1, wherein the predatory mite population has a daily reproductive rate in the range of about 1.15-1.20.
3. The method of claim 1, wherein the composition is absent a fungal reducing agent.
4. The method of claim 1, wherein the predatory mite species is selected from the group consisting of phytoseiid strawberry, phytoseiid longus, phytoseiulus macropilis, phytoseiid chilo and phytoseiid Robert.
5. The method of claim 1, wherein the species from the valve-less subgenera belongs to a family selected from the group consisting of fruit acaridae, wheat food acaridae, pinctada and confectionery acaridae.
6. The method of claim 1, wherein the valveless sub-order predator population comprises nonviable spider mite eggs.
7. The method of claim 1, wherein the composition further comprises a carrier, such as sawdust, bran, or another carrier material.
8. The method according to claim 1, wherein the predator population raised on the mite species from the valveless sub-purpose is bred at an average rate in the range of at least about 15% per day, in particular 15% to 25% per day.
9. The method of claim 1, wherein the valveless subgenomic individual is treated by a treatment selected from the group consisting of heat treatment such as freezing, heating, cold shock or heat shock treatment, chemical treatment such as gas or smoke treatment, radiation treatment such as UV, microwave, gamma radiation or X-ray treatment, mechanical treatment such as vigorous shaking or stirring, subjected to shear force, impact, pneumatic treatment such as ultrasonic treatment, pressure change, pressure drop, electrical treatment such as electrocution, immobilization by choking or oxygen deficient treatment such as by temporarily eliminating oxygen from the atmosphere or replacing oxygen with another gas, and any combination thereof.
10. Use of a composition comprising a predatory mite population comprising at least one mite species of the genus phytoseiidae and a predatory mite population comprising at least one individual from the group of valve-less sub-destination mite species, wherein the predatory mite population is capable of spawning for at least 2 generations, further wherein the valve-less sub-destination predator is selected from the group consisting of non-viable mites, non-viable eggs, and combinations thereof, for controlling crop pests.
11. Use according to claim 10, wherein the crop pest is selected from mite pests, in particular members of the family acaridae, such as tetranychus urticae, more particularly tetranychus species, in particular tetranychus, panonychus and other various mite species.
12. The use according to claim 11, wherein the crop is selected from greenhouse crops, field crops, vegetables, ornamental plants, fruit trees, hops, cotton and strawberries.
13. A biocontrol agent (BCA) for controlling crop pests comprising a mixture of: a) At least one predatory mite species of the genus phytoseiid reared by a method according to any one of claims 1 to 9, B) Optionally, an eater mite individual comprising at least one species from the order of the valve-less, the order of the valve-less individual selected from the group consisting of non-viable mites, non-viable eggs, and combinations thereof, and C) A carrier material.
14. A container containing the BCA of claim 13, the container configured to be hung on a crop plant, the container comprising an exit aperture from which the predatory mites slowly and continuously release to the crop during a period of about three weeks.
15. The container of claim 14, wherein the container is selected from the group consisting of a pouch, a packet, a pouch, a pocket, a bag, a bottle, and a bag.

Description

Novel method for feeding and controlled release of biological control agents The application is a divisional application of China patent application 201980016699.1 of which the application date is 2019, 3, 5, which is a novel method for feeding and controlling release of biological control agents. Technical Field The present invention relates to the field of biocontrol agents for crop protection and more particularly to novel means and methods for feeding biocontrol agents for plant pests. Background The use of arthropods (insects and acarids) as biocontrol agents (BCAs) is an extended field with many advantages over chemical pest control. Arthropod BCA is capable of naturally controlling other arthropod species acting as pests on crops. The phytoseiidae genus (Phytoseiulus) is a genus of phytoseiidae (Phytoseiidae). Such predatory mites are the most commonly used for controlling spider mites in greenhouse and outdoor crops grown in mild environments. Phytoseiidae mites consume up to seven adult spider mites or tens of their eggs in a day. A well fed female had about 50 eggs laid during her lifetime. The genus Phytoseius contains four known species, namely Phytoseius wisdom (P. persimilis), phytoseius longus (P. longipes), P. macropilis and Phytoseius strawberry (P. fragariae) (Chant and McMurtry 2006). All species of the genus phytoseiid are considered predators of type 1, i.e. highly predatory to diet consisting of spider mites, preferably spider mites of the genus spider mite (McMurtry and Croft 1997). The most common species of this genus for biological control of spider mites is phytoseiid chilo. The Phytoseius wisdom (Phytoseiulus persimilis, P. persimilis) adult is bright red-orange with a long foot and pear shape (about 0.5mm long). Phytoseius wisdom is considered a predator (specialist) of Tetranychus (mite of Tetranychidae) which is a plant-feeding mite (Helle and Sabelis1985, gerson et al 2003). Gerson et al 2003 states in particular that "members of the genus Phytoseius survive and place their eggs almost exclusively within the reticulum colonies of the genus Tetranychus (members of the genusPhytoseiuluslive and place their eggs almost exclusively within the webbed colonies ofTetranychusspp)".Gerson et al 2003 further states that" if other predators are present on the same plant, the predatory properties of Phytoseius Chirseii to the Tetranychus predators may be a disadvantage (the specificity ofP. persimilisfor spider mite prey can be a disadvantage if other predators are present on the same plants)". It was found that phytoseiid chilo may develop and possibly reproduce on another plant-feeding (plant-fed) mite, primeverus (Steneotarsonemus pallidus) of the family of tarsoneidae (familyTarsonemidae) (Simmonds, s.p., 1970). From a commercial point of view, a significant disadvantage of producing mites that are exclusively feeding on plants, predatory mites such as spider mites, is that they require feeding on plants with predatory mites, which have high costs. Walker and Schausberger, 1999, examine the intraspecies and interspecific predators of the more general eater (generalist) neoseiulus californicus (Neoseiulus californicus) and the specializer (specialist) phytoseiulus homo sapiens of adult females and immature stages. Adult females and immature predators of both predators are reported to exhibit higher predatory rates for larvae than eggs and first predatory mites. It was found that the predatory ratio of neoseiulus californicus to phytoseiulus chile was more severe than the opposite. The rate of predation of the same species of individuals (conspecifics) by phytoseiid Chile is reported to be higher than the rate of predation of the different species of individuals (heterospecifics) and to be more likely to be eaten by the same species than the rate of neoseiid California. In addition, it is reported that when a phytoseiid mite is provided to a predator, phytoseiid chile suffers from a higher mortality rate than neoseiid californicus. Walzer and Schausberger,1999 further teach that females of phytoseiid chile are unable to maintain oviposition, whether by recipients of the same or different species. Furthermore, when feeding on the same larvae, the mortality rate of the immature phytoseiid Chirseii is lower than that of the xenogeneic larvae. These authors concluded that none of the xenogeneic and allogeneic predators provided adequate nutrition for continued reproduction in the case of phytoseiid chile. Yao and Chant (1989) support this, which reports that the phytoseiid mentagrophytes do not produce eggs when the same species is fed or predatory Iphyseius degenerans in the immature population. In this study, only two females lay a single egg when the same species of individuals are fed the same species. In summary, it was found that phytoseiid chili was able to develop on young predatory mites, new small seiid california and Iphyseius degenerans of the phytoseiidae family. However, when