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CN-121970730-A - Instant silkworm chrysalis and preparation method thereof

CN121970730ACN 121970730 ACN121970730 ACN 121970730ACN-121970730-A

Abstract

The application relates to instant silkworm chrysalis and a preparation method thereof. The method comprises the steps of inoculating activated cordyceps militaris bacteria into a liquid culture medium for expansion culture to prepare cordyceps militaris bacteria liquid, inoculating the cordyceps militaris bacteria liquid into silkworm chrysalis bodies, sequentially carrying out rigidification treatment and fermentation culture to obtain fermented silkworm chrysalis, wherein the temperature of the rigidification treatment is 15-17 ℃, the time of the rigidification treatment is 4-6 d, the temperature of the fermentation culture is 20-23 ℃, the time of the fermentation culture is 8-11 d, and the temperature of the fermented silkworm chrysalis is 100-120 ℃ after high-temperature treatment or freezing treatment and cooking. The instant silkworm chrysalis is prepared by synchronously realizing deodorization and desensitization, nutrition modification, active ingredient enrichment and flavor improvement.

Inventors

  • MU LIXIA
  • ZOU YUXIAO
  • WANG SIYUAN
  • WANG WEIFEI
  • LIU FAN
  • XING DONGXU

Assignees

  • 广东省农业科学院蚕业与农产品加工研究所

Dates

Publication Date
20260505
Application Date
20260318

Claims (10)

  1. 1. The preparation method of the instant silkworm chrysalis is characterized by comprising the following steps of: inoculating the activated cordyceps militaris to a liquid culture medium for expansion culture to prepare cordyceps militaris bacterial liquid; Inoculating the Cordyceps militaris bacterial liquid into the silkworm chrysalis body, sequentially carrying out rigidification treatment and fermentation culture to obtain fermented silkworm chrysalis, wherein the temperature of the rigidification treatment is 15-17 ℃, the time of the rigidification treatment is 4-6 d, the temperature of the fermentation culture is 20-23 ℃, the time of the fermentation culture is 8-11 d, and And (3) performing high-temperature treatment or freezing treatment on the fermented silkworm chrysalis, and cooking to prepare the instant silkworm chrysalis, wherein the temperature of the high-temperature treatment is 100-120 ℃.
  2. 2. The method for preparing instant silkworm chrysalis according to claim 1, wherein the silkworm chrysalis inoculated with the cordyceps militaris bacterial liquid is subjected to rigidification treatment for 4-6 d under the environment humidity of 60% -65%; And/or the high-temperature treatment time is 3-8 min.
  3. 3. The method for preparing the instant silkworm chrysalis according to any one of claims 1 to 2, wherein the conditions of fermentation culture comprise fermentation culture for 7d to 9d at a temperature of 20 ℃ to 23 ℃ and an environmental humidity of 60% -70% under a dark condition, and then the fermentation culture is changed into illumination culture for 36h to 48h under the same temperature and humidity.
  4. 4. The method for preparing instant silkworm chrysalis according to any one of claims 1-2, characterized in that the method for preparing activated cordyceps militaris comprises the following steps: Inoculating Cordyceps militaris strains to be activated into a slant culture medium, and performing activation culture for 7-10 d at the temperature of 22-25 ℃ to prepare the activated Cordyceps militaris strains.
  5. 5. The method for preparing the instant silkworm chrysalis according to any one of claims 1-2, wherein the condition of the enlarged culture comprises the steps of standing culture for 20-28 h at 20-22 ℃, and then shake culture for 3-5 d at 24-25 ℃ and at a shake speed of 120-180 r/min.
  6. 6. The method for preparing instant silkworm chrysalis according to any one of claims 1-2, wherein the inoculation amount of the cordyceps militaris bacterial liquid is 3% -5% based on the weight of the silkworm chrysalis.
  7. 7. The method for preparing instant silkworm chrysalis according to any one of claims 1-2, wherein the components of the liquid medium comprise 10 g/L-30 g/L glucose, 3 g/L-8 g/L peptone, 8 g/L-12 g/L yeast extract, 10 g/L-15 g/L fermented male silkworm moth powder, 0.5 g/L-3 g/L inorganic salt and 0.01 g/L-0.03 g/L vitamin.
  8. 8. The method for preparing instant silkworm chrysalis according to any of the claims 1-2, characterized in that the means of inoculation comprise injection inoculation.
  9. 9. The method for preparing instant silkworm chrysalis according to any one of claims 1-2, wherein the silkworm chrysalis comprises one or more of mulberry silkworm chrysalis, tussah silkworm chrysalis and castor silkworm chrysalis.
  10. 10. The instant silkworm chrysalis produced by the production method of any one of claims 1 to 9.

Description

Instant silkworm chrysalis and preparation method thereof Technical Field The application relates to the field of biotechnology and agricultural product processing, in particular to instant silkworm chrysalis and a preparation method thereof. Background With the improvement of the living standard of people, the attention of consumers to food nutrition and functionality is increasingly promoted. The silkworm chrysalis has extremely high nutritive value, wherein the crude protein content is up to 50% -70%, the silkworm chrysalis mainly contains globulin and albumin which are easy to digest and absorb by human bodies, contains 18 amino acids, and is rich in various vitamins, minerals and microelements. The nutritional ingredients have positive effects on bone and brain development of infants and calcium absorption of old people, can also enhance brain cell activity, improve the thinking ability and working efficiency of human bodies, and have wide eating tradition. However, the industrialized development of silkworm chrysalis still faces the core bottlenecks of palatability and safety, the silkworm chrysalis has special fishy and mutton smell, and partial crowds have allergic reactions with different degrees on the different proteins contained in the silkworm chrysalis, so that the coverage of consumer groups is severely limited. In addition, the traditional silkworm chrysalis processing mostly adopts a simple curing and seasoning process, and although the taste can be partially improved, the silkworm chrysalis cannot be fundamentally deodorized and desensitized, and the added value of the product is low. Thus, the conventional technology has yet to be improved. Disclosure of Invention Based on the above, the application provides the instant silkworm chrysalis and the preparation method thereof, wherein the preparation method can synchronously realize deodorization and desensitization, nutrition and function enhancement of the silkworm chrysalis, and has the aroma of mushroom. The technical scheme for solving the technical problems is as follows: In one aspect of the application, a preparation method of instant silkworm chrysalis is provided, which comprises the following steps: inoculating the activated cordyceps militaris to a liquid culture medium for expansion culture to prepare cordyceps militaris bacterial liquid; Inoculating the Cordyceps militaris bacterial liquid into the silkworm chrysalis body, sequentially carrying out rigidification treatment and fermentation culture to obtain fermented silkworm chrysalis, wherein the temperature of the rigidification treatment is 15-17 ℃, the time of the rigidification treatment is 4-6 d, the temperature of the fermentation culture is 20-23 ℃, the time of the fermentation culture is 8-11 d, and And (3) performing high-temperature treatment or freezing treatment on the fermented silkworm chrysalis, and cooking to prepare the instant silkworm chrysalis, wherein the temperature of the high-temperature treatment is 100-120 ℃. In some embodiments, the silkworm chrysalis inoculated with the cordyceps militaris bacterial liquid is subjected to rigidification treatment for 4-6 d under the environment humidity of 60% -65%; And/or the high-temperature treatment time is 3-8 min. In some embodiments, the fermentation culture conditions comprise fermentation culture for 7-9 d under the conditions of 20-23 ℃ and 60-70% of ambient humidity and light shielding, and then the fermentation culture is carried out for 36-48 h under the conditions of the same temperature and humidity. In some embodiments, the method for preparing the activated cordyceps militaris comprises the following steps: Inoculating Cordyceps militaris strains to be activated into a slant culture medium, and performing activation culture for 7-10 d at the temperature of 22-25 ℃ to prepare the activated Cordyceps militaris strains. In some embodiments, the condition of the expansion culture comprises standing culture for 20-28 h at 20-22 ℃, and then shaking culture for 3-5 d at 24-25 ℃ and shaking speed of 120-180 r/min. In some embodiments, the inoculation amount of the cordyceps militaris bacterial liquid is 3% -5% based on the weight of the silkworm chrysalis. In some embodiments, the liquid medium comprises 10 g/L-30 g/L glucose, 3 g/L-8 g/L peptone, 8 g/L-12 g/L yeast extract, 10 g/L-15 g/L fermented male silkworm moth powder, 0.5 g/L-3 g/L inorganic salt and 0.01 g/L-0.03 g/L vitamin. In some of these embodiments, the means of inoculation comprises injection inoculation. In some embodiments, the silkworm chrysalis comprises one or more of mulberry silkworm chrysalis, tussah silkworm chrysalis and castor silkworm chrysalis. The application also provides the instant silkworm chrysalis prepared by the preparation method of the instant silkworm chrysalis. According to the application, the activated cordyceps militaris strain is subjected to expansion culture and short-term fermentation culture, so that a proper