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CN-121970742-A - Aquatic organism immersed sample fixing liquid, fixing assembly, aquatic organism immersed sample preparation method and application

CN121970742ACN 121970742 ACN121970742 ACN 121970742ACN-121970742-A

Abstract

The invention discloses an aquatic organism immersed sample fixing liquid, a fixing component, a preparation method and application of the aquatic organism immersed sample fixing liquid, wherein the aquatic organism immersed sample fixing liquid comprises, by mass, 20-30 parts of white oil, 50-60 parts of paraffin oil, 15-25 parts of silicone oil, 1-2 parts of menthol and 1-2 parts of camphor. According to the invention, several mineral oils which are safe and nontoxic in daily industry are introduced as main components of the fixing liquid, and menthol and camphor with anti-corrosion and color-retaining functions are added at the same time, so that the fixing liquid has the effects of fixing and anti-corrosion, and in addition, the refractive indexes of the fixing liquid and the glass support can be improved to be similar to each other, so that the effect of hiding the support is achieved. The prepared aquatic organism immersed sample has the characteristics of safety, innocuity, difficult volatilization, lower cost, easy configuration, safe interaction, high permeability and artistic appreciation, and can be better used for exhibition, science popularization and entertainment of small and medium-sized aquatic organisms.

Inventors

  • ZHANG ANQI
  • DING YUANYUAN
  • WANG HAIMING
  • GAO LONGBO

Assignees

  • 青岛海洋科技馆(青岛海产博物馆、青岛水族馆)

Dates

Publication Date
20260505
Application Date
20260127

Claims (10)

  1. 1. The aquatic organism immersed specimen fixing liquid is characterized by comprising, by mass, 20-30 parts of white oil, 50-60 parts of paraffin oil, 15-25 parts of silicone oil, 1-2 parts of menthol and 1-2 parts of camphor.
  2. 2. The aquatic organism immersed sample fixing solution according to claim 1, wherein the white oil is food grade white oil, the paraffin oil is medical paraffin oil, and the silicone oil is high refractive index silicone oil 259A.
  3. 3. The aquatic organism immersed sample fixing solution according to claim 1, wherein the fixing solution is prepared by heating silicone oil to 40-50 ℃ in a water bath, adding menthol and camphor, stirring thoroughly until dissolving, stopping heating, adding paraffin oil and white oil, stirring uniformly, and obtaining the high refractive index fixing solution.
  4. 4. The aquatic organism immersed sample fixing assembly is characterized by comprising, by mass, 20-30 parts of white oil, 50-60 parts of paraffin oil, 15-25 parts of silicone oil, 1-2 parts of menthol and 1-2 parts of camphor, wherein the refractive indexes of the fixing rod and the fixing liquid are similar or the same.
  5. 5. The aquatic specie fixative of claim 4, wherein the fixative, fixative line and fixative bar are colorless and transparent, preferably the fixative bar is a 0.6cm ultra white glass bar and the fixative line is a No. 0.4 fish line.
  6. 6. A method for producing an aquatic organism immersed sample, comprising pretreating an aquatic organism material to be immobilized to obtain a pretreated sample, and immobilizing the pretreated sample by using the immobilization liquid according to any one of claims 1 to 3 or the immobilization unit according to any one of claims 4 to 5.
  7. 7. The method according to claim 6, wherein the preparation of the pretreated specimen comprises immersing the aquatic organism material to be immobilized by alcohol-glycerin gradient immersion method to obtain the pretreated specimen; Preferably, the preparation of the pre-treated specimen comprises the steps of: (1) Cleaning the aquatic organism material to be immobilized with water, and soaking with medical alcohol for 30 min; (2) Preparing 2-3 parts of medical alcohol and 1-2 parts of glycerol into a mixed solution, soaking the aquatic organism material to be immobilized treated in the step (1), and standing for 1 day in an environment of 4 ℃; (3) Preparing 1-2 parts of medical alcohol and 1-2 parts of glycerol into mixed solution, and standing for 2 days in an environment of 4 ℃ after all the mixed solution in the step (2) is replaced; (4) Preparing 1-2 parts of medical alcohol and 2-3 parts of glycerol into mixed solution, and standing for 3 days in an environment of-18 ℃ after the mixed solution in the step (3) is completely replaced; (5) And (3) completely replacing the mixed solution in the step (4) by glycerol, standing for 5 days in an environment of-18 ℃, and finishing the pretreatment of the specimen.
  8. 8. The method according to claim 6, wherein the steps of preparing the finished product of the aquatic organism immersed sample are as follows: (1) Taking out a pretreated specimen obtained by adopting an alcohol-glycerol gradient soaking method for drying, and ensuring that no glycerol remains on the surface; (2) Fixing the specimen in the step (1) at a proper position on an ultra-white glass rod with the diameter of 0.6cm by using a No. 4 fish wire, and placing the specimen in a container; (3) Adding high-refractive-index fixing liquid into the container in the step (2), observing the glass rod according to the conditions, and fine-adjusting the components of the fixing liquid according to the actual conditions so that the refractive indexes of the fixing rod and the fixing liquid are similar or the same; (4) Sealing to obtain the finished product of the aquatic organism immersed specimen.
  9. 9. The method according to claim 8, further comprising, if the specimen has a complex structure and air bubbles exist between a part of the specimen and the fixing liquid, placing the specimen in a sealed space under room temperature conditions, maintaining an environmental pressure of 80kpa to promote the precipitation of air bubbles in the specimen, and sealing to obtain a finished product of the aquatic organism immersed specimen.
  10. 10. Use of a immersed sample produced by the method for producing an aquatic organism immersed sample according to any one of claims 6 to 9 in exhibition and exhibition of small and medium aquatic organisms.

Description

Aquatic organism immersed sample fixing liquid, fixing assembly, aquatic organism immersed sample preparation method and application Technical Field The invention relates to the technical field of preparation of aquatic organism immersed samples, in particular to an aquatic organism immersed sample fixing liquid, a fixing assembly, a preparation method and application of an aquatic organism immersed sample. Background The specimen is one of the most important display contents of the natural museum and plays multiple roles of scientific research, scientific popularization, entertainment and the like. In recent years, with the development of modern natural museums, the ecological environment group display content composed of a plurality of different biological samples has become a main mode of specimen display, but in marine biological groups, a great part of specimens are subject to the problems of manufacturing conditions, preservation conditions and the like, and can only be preserved and displayed in the form of immersed specimens. The traditional immersed sample is soaked by liquid medicine, so that the problems of color deficiency, stiff biological form of the sample, lack of landscaping combination, single form and the like often exist, and the display effect is difficult to cause the resonance of visiting masses. The existing formulation of the immersed sample fixing liquid is many, but the existing formulation is mainly developed in the fields of solving the color retention problem (such as fish, plants and the like) of the fixed group samples, the biological sample corrosion prevention problem, the development of low-toxicity or nontoxic corrosion prevention liquid and the like, but the conventional immersed sample fixing liquid has the problems of high toxicity, high configuration difficulty, high cost and the like for the immersed samples used for popular science display, so that the development of the immersed sample fixing liquid which not only ensures the corrosion prevention effect, but also satisfies the high safety and low cost and is convenient to configure is urgent to be developed. Disclosure of Invention The invention aims to overcome the problems in the prior art and provides a fixing liquid and a fixing assembly for an aquatic organism immersed sample, a preparation method and application of the aquatic organism immersed sample. The invention solves the technical problems by adopting the following technical scheme. The invention provides an aquatic organism immersed sample fixing solution which comprises, by mass, 20-30 parts of white oil, 50-60 parts of paraffin oil, 15-25 parts of silicone oil, 1-2 parts of menthol and 1-2 parts of camphor. The invention also provides an aquatic organism immersed sample fixing component which comprises a fixing liquid, a fixing line and a fixing rod, wherein the fixing liquid comprises, by mass, 20-30 parts of white oil, 50-60 parts of paraffin oil, 15-25 parts of silicone oil, 1-2 parts of menthol and 1-2 parts of camphor, and the refractive indexes of the fixing rod and the fixing liquid are similar or identical. The invention also provides a preparation method of the aquatic organism immersed sample, which comprises the steps of preprocessing the aquatic organism material to be immobilized to obtain a preprocessed sample, and fixing the preprocessed sample by using the fixing liquid or the fixing component. The invention also provides application of the immersed sample manufactured by the aquatic organism immersed sample manufacturing method in exhibition and exhibition of medium-sized and small-sized aquatic organisms. The invention has the following beneficial effects: According to the invention, a low-viscosity mineral oil component is selected as a substrate, mineral oil with high refractive index characteristic is added as a blending component, the obtained high-refractive index fixing liquid can enable a specimen to show a visual effect of suspending in water, in addition, the hiding and supporting effects of the fixing liquid can be adjusted according to the actual refractive index of a customized support glass rod, the effect of hiding the support is achieved, the original shape of aquatic organisms is basically reduced, and the effects of preserving and keeping color are achieved while the specimen is fixed by adding the preservative menthol and camphor. The immersed sample manufactured by the fixing liquid has the characteristics of safety, innocuity, low cost, easy configuration, safe interaction and the like, is not easy to volatilize, can better exert multiple functions of scientific research, scientific popularization, entertainment and the like of the sample in the natural museum, and improves the display and exhibition effects. Drawings In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being und