CN-121970745-A - Donor heart myocardial protection system and method

CN121970745ACN 121970745 ACN121970745 ACN 121970745ACN-121970745-A

Abstract

The application relates to the technical field of heart transplantation and organ preservation, and discloses a donor heart myocardial protection system and method. The system comprises a heart accommodating unit, a thermal buffering isolation unit and an external low-temperature unit, wherein the heart accommodating unit is used for accommodating a donor heart and heart preservation liquid and suspending the donor heart in the heart preservation liquid, the thermal buffering isolation unit is used for accommodating the heart accommodating unit, a low-temperature buffering medium is arranged in the thermal buffering isolation unit, the heart accommodating unit is separated from the inner wall of the thermal buffering isolation unit through the low-temperature buffering medium, and the external low-temperature unit is used for accommodating the thermal buffering isolation unit and providing an external low-temperature environment. The application effectively reduces the spatial temperature gradient between different parts of the donor heart through a suspension and thermal buffering mechanism, and avoids local supercooling or freezing. The method is simple, does not depend on complex equipment, and is easy to popularize and apply in the existing clinical cold preservation process.

Inventors

LU SHUYANG
YANG SHOUGUO
WANG CHUNSHENG
CHENG LEILEI
LIU XIAO
QIU SHOUJI

Assignees

复旦大学附属中山医院

Dates

Publication Date: 20260505
Application Date: 20260304

Claims (9)

1. A donor heart myocardial preservation system, comprising: a heart containing unit for containing a donor heart and a heart preservation liquid and suspending the donor heart in the heart preservation liquid; A thermal buffer isolation unit for accommodating the heart accommodating unit, wherein a low-temperature buffer medium is arranged in the thermal buffer isolation unit, and the heart accommodating unit is separated from the inner wall of the thermal buffer isolation unit by the low-temperature buffer medium; An external cryogenic unit for housing the thermal buffer isolation unit and providing an external cryogenic environment.
2. The donor heart myocardial protection system of claim 1 wherein the heart containment unit and the thermal buffer isolation unit are both medical containment vessels.
3. The donor cardiac myocardial protection system as recited in claim 1 wherein the low temperature buffer medium is crushed ice, an ice-water mixture, or a low temperature phase change material.
4. The donor heart myocardial protection system of claim 1 wherein a suspension assembly is disposed within the heart containment unit for supporting a donor heart and suspending the donor heart in a heart preservation fluid.
5. The donor heart myocardial protection system of claim 4 wherein the suspension assembly is a flexible mesh structure or a multi-point flexible support structure.
6. The donor heart myocardial protection system of claim 1, further comprising a temperature monitoring unit comprising a temperature sensor disposed in the donor heart, the heart preservation fluid, and/or the low temperature buffer medium.
7. A method of protecting the myocardium of a donor heart using the system for protecting the myocardium of a donor heart according to any of claims 1-6, comprising the steps of: step 1, placing a donor heart subjected to preservation solution perfusion treatment in a heart accommodating unit, and injecting heart preservation solution to enable the donor heart to be completely suspended; step 2, placing a heart accommodating unit suspending a donor heart into a thermal buffer isolation unit, and filling a low-temperature buffer medium around the heart accommodating unit; And 3, placing the thermal buffer isolation unit filled with the low-temperature buffer medium in an external low-temperature unit for cold preservation.
8. The method of claim 7, further comprising the step of monitoring the temperature of the donor heart, heart preservation fluid and/or cryogenic buffer medium during cold preservation by a temperature monitoring unit to detect temperature changes.
9. Use of the donor heart myocardial preservation system of any one of claims 1-6, or the donor heart cold-preservation method of any one of claims 7-8, in the preparation of a donor heart myocardial preservation product.

Description

Donor heart myocardial protection system and method Technical Field The application relates to the technical field of heart transplantation and organ preservation, in particular to a donor heart myocardial protection system and method. Background Cold preservation of the donor heart is one of the most common organ protection modes in the current heart transplantation process, and the basic principle is to delay ischemic injury by reducing myocardial metabolic level. Traditional cold preservation methods typically employ a cardiac preservation fluid (e.g., HTK, UW, etc.) to preserve the donor heart in a low temperature environment after perfusion shutdown, and maintain the low temperature state during transport. Existing cold preservation techniques are generally focused on the target temperature interval, for example, it is generally believed that maintaining the donor heart at about 4-8 ℃ contributes to myocardial preservation. However, in actual clinical practice and during transportation, the donor heart is mostly cooled by ice cubes or ice-water mixtures, the temperature distribution in different areas inside and on the surface is difficult to control accurately, and obvious spatial temperature gradients and temporal temperature fluctuations often exist. During cold preservation, the local area of the donor heart may be supercooled, frozen or rapidly changed in temperature due to direct contact with an ice source or uneven cooling, so that the problems of local myocardial cold injury, mitochondrial dysfunction, calcium steady state disturbance and the like are induced, and the functional recovery after cardiac resuscitation is finally affected. The prior art generally ignores the effect of temperature profile (especially temperature uniformity and temperature gradient) on myocardial protection during cold preservation, and lacks a systematic solution to this problem. Therefore, a new technology for protecting the heart muscle in cold preservation is needed, and from the standpoint of regulating the temperature distribution state of the heart of the donor in the cold preservation process, the temperature gradient and the local cold injury are reduced, so that the protection effect of the heart of the donor under the long-time cold preservation condition is improved. Disclosure of Invention The application aims to solve the technical problems that the existing cold preservation method in the existing cold preservation technology mainly focuses on the whole low-temperature environment and can not effectively control the temperature gradient between different parts of a donor heart, the local area of the donor heart is easy to be supercooled or frozen in the cold preservation process, so that the cold preservation related myocardial damage is caused, and the existing method is difficult to stably realize the good protection of the myocardial structure and function under the long-time cold preservation condition. In order to achieve the above-mentioned object, the present application provides a method and a system for protecting cardiac muscle of a donor heart, so as to reduce the temperature gradient of the donor heart in space and time dimensions by constructing a uniform and stable low-temperature thermal buffer environment under the condition of cold preservation, thereby reducing the damage of cardiac muscle related to cold preservation and improving the function recovery effect of the donor heart after resuscitation. In a first aspect, the present application provides a donor heart myocardial preservation system comprising: a heart containing unit for containing a donor heart and a heart preservation liquid and suspending the donor heart in the heart preservation liquid; A thermal buffer isolation unit for accommodating the heart accommodating unit, wherein a low-temperature buffer medium is arranged in the thermal buffer isolation unit, and the heart accommodating unit is separated from the inner wall of the thermal buffer isolation unit by the low-temperature buffer medium; An external cryogenic unit for housing the thermal buffer isolation unit and providing an external cryogenic environment. Further, the heart accommodating unit and the thermal buffering isolation unit are both medical closed containers. Further, the low-temperature buffer medium is crushed ice, an ice-water mixture or a low-temperature phase change material. Further, a suspension assembly is disposed within the heart containment unit for supporting and suspending the donor heart in a heart preservation fluid. Further, the suspension assembly is a flexible mesh structure or a multi-point flexible support structure. Further, the system also includes a temperature monitoring unit including a temperature sensor disposed in the donor heart, the heart preservation fluid, and/or the cryogenic buffer medium. In a second aspect, the present application provides a method for protecting the myocardium of a donor heart using said system for p