CN-121970746-A - Seed preservation method of cryptosporidium mangrove plant avicennia marina

Abstract

The invention discloses a seed preservation method of cryptosporidium mangrove plant avicennia marina. The method comprises the steps of (1) selecting healthy and full avicennia marina seeds without diseases and insect pests, removing the outer skin, soaking and disinfecting in a carbendazim aqueous solution for 15min, then washing with clear water, airing, 2) soaking the avicennia marina seeds treated in the step (1) in a chitosan solution for 1min, taking out, airing, 3) taking wet sand as a preservation matrix, burying the avicennia marina seeds treated in the step (2) in the preservation matrix, then filling the seeds and the preservation matrix into a plastic package bag together for sealing, keeping the seeds in a buried state in the bag, and finally storing in a light-resistant environment at 14 ℃. The germination rate of the avicennia marina seeds preserved by the method can still reach 40% after 4 months, the storage time of the avicennia marina seeds is obviously prolonged, and convenience is provided for seedling cultivation and ecological restoration of the avicennia marina.

Inventors

• CHEN JIANFENG
• WU LILIN
• LIU LEMIAN
• GUO YISONG

Assignees

• 福州大学

Dates

Publication Date

20260505

Application Date

20260119

Claims (8)

1. 1. A seed preservation method of cryptosporidium mangrove plant avicennia marina is characterized by comprising the following steps: (1) Selecting healthy full avicennia marina seeds without plant diseases and insect pests, removing the outer skin, soaking and sterilizing in a carbendazim aqueous solution for 15min, then washing with clear water, and airing; (2) Soaking the avicennia marina seeds treated in the step (1) in a preservative for 1min, taking out, and airing; (3) Embedding the avicennia marina seeds treated in the step (2) into a preservation matrix, then filling the seeds and the preservation matrix into a plastic package bag together for sealing, keeping the seeds still in a buried state in the bag, and finally placing the seeds in a light-proof environment for preservation.
2. 2. The method for preserving seeds according to claim 1, wherein in the step (1), the concentration of said carbendazim aqueous solution is 2g/L and the temperature of said soaking is 25 ℃.
3. 3. The method of claim 1, wherein in the step (2), the preservative is a chitosan solution, and the soaking temperature is 25 ℃.
4. 4. The method for preserving seeds according to claim 3, wherein the chitosan solution is prepared by dissolving 15g of chitosan powder in 1vol% aqueous acetic acid solution, adding glycerol of 1vol% final concentration, and mixing uniformly.
5. 5. The method of preserving seeds according to claim 1, wherein in the step (3), the preserving substrate is wet sand and the preserving temperature is 14 ℃.
6. 6. The method for preserving seeds according to claim 5, wherein the humidity of the sand is 60%.
7. 7. Use of the seed preservation method according to any one of claims 1 to 6 for preservation of avicennia marina seeds of cryptosporidium mangrove plants.
8. 8. The use of the seed preservation method according to any one of claims 1 to 6 in artificial seedling of cryptosporidium.

Description

Seed preservation method of cryptosporidium mangrove plant avicennia marina Technical Field The invention belongs to the technical field of plant seed preservation, and particularly relates to a seed preservation method of cryptosporidium mangrove plant avicennia marina. Background Mangrove is a special plant community which grows in tropical and subtropical coastal areas and takes mangrove plants as main bodies, is mainly distributed in an intertidal zone between a high tide line and a low tide line, can grow on a shoal at the boundary of land and sea, and forms a special coastal ecological system. Along with the random discharge of sewage, random discarding of beach garbage, frequent occurrence of diseases and the development of mariculture, the area of domestic mangrove forests is reduced. Therefore, mangrove ecological restoration by using artificial seedling culture becomes an important remedy. Avicennia marina (AVICENNIA MARINA) is one of pioneer tree species for recovery and reconstruction of mangrove forests in China. The avicennia marina has developed root systems, rapid growth and strong adaptability to severe environments, is commonly found in the environments of silt or sandy soil, and can be distributed near low tide lines. The avicennia marina seeds have floating capacity, can be randomly distributed along with ocean currents, have strong soil nutrient absorption capacity, and are suitable for the tidal beaches of barren sandy lands. However, once the seeds lose water, the vigor of the seeds is lost rapidly, and the seeds begin to mould and rot in 10-20 days at normal temperature, so that great barriers are brought to cross-region seeding, seasonal seedling culture and germplasm preservation. Therefore, searching for a preservation method of avicennia marina seeds, prolonging the preservation time of the avicennia marina seeds, and is a key point of mangrove ecological restoration. For avicennia marina seed preservation, no relevant preservation method exists at present. The fresh-keeping agent is coated, sand buried and sealed, and finally the seeds are placed in a low temperature to be preserved in a dark place, so that the survival time of the avicennia marina seeds can be prolonged, and convenience is brought to follow-up avicennia marina seedling raising and planting. Disclosure of Invention The invention aims to provide a preservation method of cryptosporidium seed of mangrove plant avicennia marina. The preservation time of the avicennia marina seeds is prolonged through disinfection, preservative treatment and low-temperature sand burying preservation, the seedling raising and ecological restoration work of the avicennia marina due to rapid decay of the seeds are avoided, and the trans-regional transportation and trans-season planting of the seeds can be realized. In order to achieve the above object, the present invention provides the following technical solutions: A seed preservation method of cryptosporidium mangrove plant avicennia marina comprises the following steps: (1) Selecting healthy full avicennia marina seeds without plant diseases and insect pests, removing the outer skin, soaking and sterilizing in a carbendazim aqueous solution for 15min, then washing with clear water, and airing; (2) Soaking the avicennia marina seeds treated in the step (1) in a preservative for 1min, taking out, and airing; (3) Embedding the avicennia marina seeds treated in the step (2) into a preservation matrix, then filling the seeds and the preservation matrix into a plastic package bag together for sealing, keeping the seeds in the bag in a buried state, and finally placing the seeds in a light-proof environment for preservation; further, in the step (1), the concentration of the carbendazim aqueous solution is 2g/L, and the soaking temperature is 25 ℃; Further, in the step (2), the preservative is chitosan solution, and the soaking temperature is 25 ℃; further, the chitosan solution is prepared by dissolving 15g of chitosan powder in 1vol% acetic acid aqueous solution, adding glycerol with a final concentration of 1vol%, and uniformly mixing to obtain a chitosan solution; Further, in step (3), the preservation matrix is wet sand, and the preservation temperature is 14 ℃; Further, the sand has a moisture content of 60%. The application of the seed preservation method in the preservation of the avicennia marina seeds of the cryptosporidium mangrove plants. The seed preservation method is applied to artificial seedling raising of the cryptosporidium aquilinum plant avicennia marina. The invention has the beneficial effects that: the method of the invention gets rid of the dependence of the germination of the avicennia marina seeds on the maturing time period of the seeds, the germination rate of the preserved avicennia marina seeds can still reach 40% after 4 months, and the cross-season planting of the avicennia marina can be realized. Detailed Description The technical solutions of the present