CN-121970752-A - Application of chrysin dimethyl ether and sakurin in preventing and treating wheat scab

Abstract

The invention discloses application of chrysin dimethyl ether and sakura extract as plant pathogenic bacteria fusarium graminearum (Fusarium graminearum) bacteriostat, and application of chrysin dimethyl ether and sakura extract in preventing and treating wheat scab, wherein the chrysin dimethyl ether and sakura extract can prevent and treat the wheat scab by inhibiting hypha growth of fusarium graminearum, inhibiting biosynthesis of deoxynivalenol and/or inhibiting expression of key genes synthesized by deoxynivalenol. Both the chrysin dimethyl ether and the sakura extract are plant source natural products, and have the characteristics of low toxicity, easy degradation, high environmental compatibility and the like. The experimental data provided by the invention fully prove the effectiveness of the fusarium graminearum in inhibiting the growth and DON accumulation, is safe and harmless to wheat, can be used as a green replacement or supplement means of a chemical bactericide, provides a new solution for green prevention and control of wheat scab, and has important application value and popularization prospect.

Inventors

• JU CHAO
• WU JINGCHEN
• WEI CHUNFENG
• ZHAO YITONG
• LI LU
• Liang fudong
• Man Yanli

Assignees

• 青岛农业大学

Dates

Publication Date

20260505

Application Date

20260305

Claims (9)

1. 1. The application of chrysin dimethyl ether as a bacteriostatic agent of plant pathogenic bacteria Fusarium graminearum (Fusarium graminearum).
2. 2. Use of sakura extract as bacteriostatic agent for plant pathogenic bacteria fusarium graminearum (Fusarium graminearum).
3. 3. The application of chrysin dimethyl ether and sakurin in preventing and treating wheat scab, which is characterized in that pathogenic bacteria of the wheat scab are fusarium graminearum (Fusarium graminearum) according to claim 1 or claim 2.
4. 4. Use of chrysin dimethyl ether and sakurin for controlling wheat scab according to claim 3, characterized in that the chrysin dimethyl ether and sakurin effects control of wheat scab by inhibiting hyphal growth of fusarium graminearum, inhibiting biosynthesis of deoxynivalenol, and/or inhibiting expression of key genes for deoxynivalenol synthesis.
5. 5. The use of chrysin dimethyl ether and sakurin for controlling wheat scab according to claim 4, wherein the deoxynivalenol synthesis key gene is a TRI5 gene and/or a TRI6 gene.
6. 6. Use of coronene dimethyl ether and sakurin for controlling wheat scab according to claim 3, characterized in that the concentration of coronene dimethyl ether is not less than 25 μg/mL and the concentration of sakurin is not less than 25 μg/mL.
7. 7. A wheat scab biological inhibitor, characterized in that the effective active ingredient of the biological inhibitor comprises corolline dimethyl ether and/or sakurin.
8. 8. The wheat scab biological inhibitor of claim 7, wherein the chrysin dimethyl ether and/or sakurin is used at a concentration of not less than 25 μg/mL.
9. 9. A composition for controlling wheat scab, characterized by comprising chrysin dimethyl ether and/or sakurin as an active ingredient.

Description

Application of chrysin dimethyl ether and sakurin in preventing and treating wheat scab Technical Field The invention relates to the technical field of biology, in particular to application of chrysin dimethyl ether and sakurin in preventing and treating wheat scab. Background Wheat scab (WHEAT HEAD blight, FHB) is one of the important diseases in wheat production, and the wheat has low resistance level to the scab and fewer resistant varieties, so that the wheat scab can generally cause yield loss of more than 10%, and the yield reduction amplitude reaches 50% in severe cases. The main pathogenic bacteria of wheat scab are fusarium graminearum (Fusarium graminearum), which is susceptible to infection of the ears during the flowering phase of wheat, and in addition to greatly reducing yield, various mycotoxins are produced during infection, the most predominant of which is deoxynivalenol (Deoxynivalenol, DON). Studies have shown that DON is a virulence factor that accelerates the invasion of wheat by Fusarium graminearum, promoting the spread of scab. In addition, excessive consumption of DON-contaminated grains by humans and livestock can cause nausea, vomiting, diarrhea, abdominal pain, etc., and even cause damage to the immune system of humans and livestock and canceration of cells. In addition, DON remains highly stable under weak acidic, high temperature and high pressure conditions, which makes removal of the toxin from contaminated products exceptionally difficult. FAO and WHO have been listed as one of the most dangerous natural food pollutants due to their high toxicity and high stability. Therefore, the establishment of effective strategies to mitigate DON pollution in wheat has become a key task in guaranteeing food safety and agricultural sustainable development. The bactericide can effectively prevent and treat the infection of scab, thereby indirectly reducing the DON content in wheat ears. However, numerous in vitro experiments have shown that while certain bactericides are effective in inhibiting the growth of scab, the accumulation of DON is often not reduced, and may even be exacerbated, when the concentration is reduced to sub-lethal levels. For example, sublethal concentrations of methoxy acrylic acid ester fungicides, such as azoxystrobin, enestrobin, and fluoxastrobin, can promote the production of DON by increasing the level of acetyl-CoA and up-regulating the key genes for DON biosynthesis (Tri 5 and Tri 6). Similarly, sublethal concentrations of triazole-based bactericides (such as tebuconazole, epoxiconazole, propiconazole and prothioconazole) can inhibit sterol biosynthesis by inhibiting sterol 14 a-demethylase, resulting in lanosterol accumulation, and use of the common precursor farnesyl pyrophosphate for DON synthesis, thereby increasing DON yield. And residual chemical bactericides may be enriched in the environment or organisms, causing pollution. It is therefore important to explore other substances which are less toxic and which inhibit the growth or production of fusarium graminearum. Plants and metabolites thereof are a hot spot for researching green pesticides in recent years due to natural sources, high environmental compatibility and various action mechanisms. However, currently, natural metabolites with activity inhibiting fusarium graminearum growth or DON synthesis are still less systematically identified and used. The chrysin dimethyl ether (CHRYSIN DIMETHYLETHER) and the sakura extract (Sakuranetin) are two flavonoid compounds existing in plants and are known to have certain biological activities such as antioxidation, anti-inflammatory and the like, but no research report on the use of the chrysin dimethyl ether or the sakura extract for preventing and treating wheat scab, inhibiting the growth of fusarium graminearum or reducing the accumulation of DON toxin is not seen so far. Therefore, the application potential of the two substances in fusarium graminearum prevention and treatment is explored, and the application potential of the two substances in fusarium graminearum prevention and treatment has important scientific significance and application value for developing novel green pesticides. Disclosure of Invention The present invention aims to solve the above problems, and in a first aspect, the present invention provides the use of chrysin dimethyl ether as a bacteriostatic agent for the plant pathogen fusarium graminearum (Fusarium graminearum). Chrysin dimethyl ether (CHRYSIN DIMETHYLETHER) belongs to flavonoid substances, and has a molecular formula of C 17H14O4 and a relative molecular mass of 282.10. In a second aspect, the invention provides the use of sakura as a bacteriostatic agent for the plant pathogen fusarium graminearum (Fusarium graminearum). Sakura extract (Sakuranetin) belongs to flavonoid substances, has a molecular formula of C 16H14O5 and a relative molecular mass of 286.08. In a third aspect, the invention also provides application of the