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CN-121970753-A - Application of kaempferide and 4, 7-dimethyl naringenin in preventing and treating wheat scab

CN121970753ACN 121970753 ACN121970753 ACN 121970753ACN-121970753-A

Abstract

The invention discloses application of kaempferide and 4, 7-dimethyl naringenin in inhibition of deoxynivalenol synthesis, wherein pathogenic bacteria of wheat scab are fusarium graminearum (Fusarium graminearum) when the kaempferide and the 4, 7-dimethyl naringenin are applied to control of wheat scab. The kaempferide and the 4, 7-dimethyl naringenin can prevent and treat wheat scab by inhibiting the biosynthesis of deoxynivalenol and/or inhibiting the expression of key genes for synthesizing deoxynivalenol. The kaempferide and the 4, 7-dimethyl naringenin are both endogenous secondary metabolites of wheat, are derived from plants, have low toxicity to crops, are easy to degrade in the environment, and have no residual pollution problem of chemical bactericides. Meanwhile, as the action mechanism is different from the existing chemical bactericides, the cross resistance does not exist with the common bactericides, and the bactericide is suitable for resistance treatment and green agricultural production systems.

Inventors

  • JU CHAO
  • ZHAO YITONG
  • WEI CHUNFENG
  • WU JINGCHEN
  • LI LU
  • Liang fudong
  • Man Yanli

Assignees

  • 青岛农业大学

Dates

Publication Date
20260505
Application Date
20260305

Claims (9)

  1. 1. Application of kaempferide in inhibiting deoxynivalenol synthesis is provided.
  2. Use of 2.4,7-dimethyl naringenin in inhibiting deoxynivalenol synthesis.
  3. 3. The application of kaempferide and 4, 7-dimethyl naringenin in preventing and treating wheat scab is characterized in that pathogenic bacteria of the wheat scab are fusarium graminearum (Fusarium graminearum).
  4. 4. The use of kaempferide and 4, 7-dimethylnaringenin according to claim 3 for controlling wheat scab, wherein kaempferide and 4, 7-dimethylnaringenin achieve control of wheat scab by inhibiting biosynthesis of deoxynivalenol and/or inhibiting expression of key genes for deoxynivalenol synthesis.
  5. 5. The use of kaempferide and 4, 7-dimethyl naringenin according to claim 4 for controlling wheat scab, wherein the deoxynivalenol synthesis critical gene is a TRI5 gene and/or a TRI6 gene.
  6. 6. The use of kaempferide and 4, 7-dimethyl naringenin according to claim 3 for preventing and treating wheat scab, wherein the kaempferide is used at a concentration of not less than 30 μg/mL, and the 4, 7-dimethyl naringenin is used at a concentration of not less than 30 μg/mL.
  7. 7. A wheat scab biological inhibitor, characterized in that the effective active ingredient of the biological inhibitor comprises kaempferide and/or 4, 7-dimethyl naringenin.
  8. 8. The wheat scab biological inhibitor of claim 7, wherein kaempferide and/or 4, 7-dimethyl naringin is used at a concentration of not less than 30 μg/mL.
  9. 9. A composition for controlling wheat scab, characterized by comprising kaempferide and/or 4, 7-dimethylnaringenin as active ingredients.

Description

Application of kaempferide and 4, 7-dimethyl naringenin in preventing and treating wheat scab Technical Field The invention relates to the technical field of biology, in particular to application of kaempferide and 4, 7-dimethyl naringenin in preventing and treating wheat scab. Background Wheat scab (WHEAT HEAD blank, FHB) is a worldwide wheat disease caused by Fusarium graminearum (Fusarium graminearum). Because the resistance level of wheat to scab is generally low and the variety is lack in production, the disease causes the wheat yield loss to be more than 10% all the year round, and the yield can be reduced by up to 50% when serious occurrence happens. More seriously, in the process of infecting wheat ears, fusarium graminearum can metabolize to produce deoxynivalenol (Deoxynivalenol, DON) and other mycotoxins. DON is a virulence factor for accelerating pathogen infection and promoting disease expansion, and forms a serious threat to human and livestock health. Symptoms such as nausea, vomiting, diarrhea, etc. can occur after humans and animals ingest grains contaminated with DON, and long-term exposure can also damage the immune system and present a risk of carcinogenesis. In addition, DON has extremely high thermal stability and chemical stability, is difficult to degrade under weak acid, high temperature and high pressure conditions, and can be hardly removed by conventional processing means once the grain products are polluted. Therefore, how to effectively prevent and control wheat scab and reduce the pollution level of DON in grains has become an important topic for guaranteeing food safety and promoting agricultural sustainable development. At present, chemical bactericides are mainly relied on in production to prevent and treat scab, so that accumulation of DON is indirectly reduced. However, extensive research has found that there are significant limitations to the use of chemical fungicides. When the concentration of the bactericide is reduced to a sub-death level, DON synthesis cannot be effectively inhibited, and accumulation of DON is possibly aggravated. For example, methoxy acrylate fungicides (e.g., azoxystrobin) can up-regulate expression of key genes for DON biosynthesis (Tri 5, tri 6) by increasing acetyl CoA levels, and triazole fungicides (e.g., tebuconazole) can increase toxin production by inhibiting sterol synthesis, resulting in precursor split into DON synthesis pathways. Furthermore, the problem of chemical sterilant residues is also increasingly pronounced, with their enrichment effects in the environment and organisms presenting a potential ecological risk. Therefore, there is a need to find new active substances which have lower toxicity, better environmental compatibility and are capable of simultaneously inhibiting the growth of fusarium graminearum or its toxin-producing ability. In recent years, secondary metabolites of plant or microbial origin have been attracting attention due to their low toxicity and environmental protection properties. Studies have shown that some natural products such as thymol inhibit growth of Fusarium graminearum, and that benzoxazines, flavonoids, etc. inhibit DON accumulation. However, these natural products are of a wide variety, and only a few have been demonstrated to have Fusarium bactericidal or toxin inhibitory activity, far from meeting the needs of green pesticide development. In particular, research on wheat endogenous flavonoids such as Kaempferide (KAEMPFERIDE) and 4, 7-dimethyl naringenin (4, 7-Di-O-METHYLNARINGENIN) in inhibiting fusarium graminearum toxin production has not been reported. Therefore, the method screens more substances with antibacterial or toxin-inhibiting activity from plant endogenous metabolites, especially the novel application of digging natural flavonoid compounds such as kaempferide and 4, 7-dimethyl naringenin, and has important practical significance for developing novel green pesticides and guaranteeing grain safety. Disclosure of Invention The present invention aims to solve the above problems, and in a first aspect, the present invention provides an application of kaempferide in inhibition of deoxynivalenol synthesis. Kaempferide (KAEMPFERIDE) belongs to flavonoid substances, and has a molecular formula of C 16H12O6 and a relative molecular mass of 300.06. In a second aspect, the invention provides the use of 4, 7-dimethylnaringenin for inhibiting deoxynivalenol synthesis. 4, 7-dimethyl naringenin (4, 7-Di-O-METHYLNARINGENIN) belongs to flavonoid substances, and has a molecular formula of C 17H16O5 and a relative molecular mass of 300.10. In a third aspect, the invention provides application of kaempferide and 4, 7-dimethyl naringenin in preventing and treating wheat scab, wherein a pathogenic bacterium of the wheat scab is fusarium graminearum (Fusarium graminearum). Furthermore, the kaempferide and the 4, 7-dimethyl naringenin can prevent and treat wheat scab by inhibiting the biosynthesis of deox