CN-121970767-A - Application of haline in preventing and treating wheat scab

Abstract

The invention discloses application of haline as a plant pathogen fusarium graminearum (Fusarium graminearum) bacteriostatic agent, and application of haline in preventing and treating wheat scab, wherein the haline realizes preventing and treating the wheat scab by inhibiting mycelium growth of fusarium graminearum, inhibiting biosynthesis of deoxynivalenol and/or inhibiting expression of key genes for synthesizing the deoxynivalenol. The invention proves that Ha Erjian has remarkable antibacterial activity on main pathogenic bacteria fusarium graminearum of wheat scab for the first time, and the half inhibition concentration (EC 50 ) is 56.71 mug/mL. Meanwhile, ha Erjian can remarkably inhibit the biosynthesis of DON toxin, the inhibition rate of DON synthesis can reach 25.04% under the concentration of EC 50 , the inhibition rate of DON content in wheat leaves can reach 46.30%, and good antibacterial and toxin-inhibiting dual effects are shown. The invention expands the haar alkali to the field of agricultural disease control for the first time, and provides a new active material source and technical path for green control of wheat scab.

Inventors

• JU CHAO
• WEI CHUNFENG
• WU JINGCHEN
• ZHAO YITONG
• LI LU
• Liang fudong
• Man Yanli

Assignees

• 青岛农业大学

Dates

Publication Date

20260505

Application Date

20260305

Claims (8)

1. 1. The use of haline as a bacteriostatic agent for the plant pathogenic fungus fusarium graminearum (Fusarium graminearum).
2. 2. The application of haer alkali in preventing and treating wheat scab, which is characterized in that the pathogenic bacteria of the wheat scab are fusarium graminearum (Fusarium graminearum) as claimed in claim 1.
3. 3. The use of haline according to claim 2 for controlling wheat scab, wherein the haline effects control of wheat scab by inhibiting hyphal growth of fusarium graminearum, inhibiting biosynthesis of deoxynivalenol, and/or inhibiting expression of a key gene for deoxynivalenol synthesis.
4. 4. The use of haline according to claim 2 for controlling wheat scab, wherein the deoxynivalenol synthesis critical gene is the TRI5 gene and/or the TRI6 gene.
5. 5. The use of haline according to claim 2 for controlling wheat scab, wherein the haline is used at a concentration of not less than 56.71 μg/mL.
6. 6. A wheat scab biological inhibitor, characterized in that the effective active ingredient of the biological inhibitor comprises Ha Erjian.
7. 7. The wheat scab biological inhibitor of claim 6, wherein the haar alkali content of the biological inhibitor is not less than 56.71 μg/mL.
8. 8. The wheat scab biological inhibitor of claim 6, wherein the biological inhibitor is administered by foliar spray.

Description

Application of haline in preventing and treating wheat scab Technical Field The invention relates to the field of biotechnology, in particular to application of haline in preventing and treating wheat scab. Background Wheat scab (WHEAT HEAD blight, FHB) is a worldwide wheat disease caused by fusarium graminearum (Fusarium graminearum), severely affecting wheat yield and quality. Not only does this disease result in reduced wheat yield, but more seriously, fusarium graminearum produces various mycotoxins during infection, with deoxynivalenol (Deoxynivalenol, DON) being the most common and most harmful. DON has high toxicity, high stability and difficult removal, and can be remained in grains for a long time, and can cause health problems such as emesis, immunosuppression, intestinal injury, etc. after being ingested by human and livestock. Therefore, DON has been listed by the United nations grain and agricultural organization and the world health organization as an important monitoring object in natural food pollutants. Currently, prevention and control of wheat scab mainly depends on chemical bactericides such as triazole and methoxy acrylic reagents. However, the long-term use of a large amount of chemical agents brings a plurality of problems that firstly, part of agents are used for stimulating expression of DON synthesis key genes (such as TRI5 and TRI 6) under the condition of sublethal concentration to increase accumulation of DON, secondly, the residual agents form potential risks for environment and food safety, and thirdly, pathogenic bacteria are easy to generate drug resistance to influence the control effect. Therefore, development of a novel green pesticide capable of effectively inhibiting growth of fusarium graminearum and reducing accumulation of DON becomes an important direction of current wheat scab prevention and control research. The plant source natural metabolite becomes an important resource for developing a novel bactericide because of the advantages of wide sources, various structures, good environmental compatibility, novel action mechanism and the like. Studies have shown that some plant secondary metabolites such as thymol, flavonoids, alkaloids etc. have a certain inhibitory effect on Fusarium graminearum. However, the active substances reported so far are still limited, and more natural products with dual functions of bacteriostasis and toxin inhibition are required to be discovered. Ha Erjian (Harmine) is a natural beta-carboline alkaloid, widely exists in various plants such as peganum harmala (Peganum harmala) and has various biological activities such as antioxidation, anti-inflammatory and anti-tumor. However, through literature search, research reports on the inhibition effect of Ha Erjian on fusarium graminearum are not found at present, and related researches on the fusarium graminearum for preventing and treating wheat scab or inhibiting DON toxin synthesis are not found. The invention starts from plant endogenous metabolites, discovers and verifies the inhibition effect of haer alkali on fusarium graminearum hypha growth, DON synthesis and key gene expression thereof for the first time, and provides a new technical scheme for green prevention and control of wheat scab. Disclosure of Invention The present invention aims to solve the above problems and in a first aspect provides the use of haline as a bacteriostatic agent for the plant pathogenic fungus fusarium graminearum (Fusarium graminearum). In a second aspect, the invention provides the use of haline for controlling wheat scab, the pathogenic bacteria of which are fusarium graminearum (Fusarium graminearum) of the first aspect. Furthermore, the haline can prevent and treat wheat scab by inhibiting the hypha growth of fusarium graminearum, inhibiting the biosynthesis of deoxynivalenol and/or inhibiting the expression of key genes synthesized by Deoxynivalenol (DON). Further, the key gene for synthesizing Deoxynivalenol (DON) is a TRI5 gene and/or a TRI6 gene. Further, the Harbin alkali is used at a concentration of not less than 56.71 μg/mL. In a third aspect, based on the same invention, the invention also provides a wheat scab biological inhibitor, and the effective active ingredient of the biological inhibitor comprises Ha Erjian. Ha Erjian (Harmine) belongs to alkaloid substance, and has molecular formula of C 13H12N2 O and relative molecular weight of 212.10. Further, the content of haline in the biological inhibitor is not less than 56.71 mug/mL. Further, the biological inhibitor is used by leaf spraying. The invention has the following beneficial effects: 1. The invention proves that Ha Erjian has remarkable antibacterial activity on main pathogenic bacteria fusarium graminearum of wheat scab for the first time, and the half inhibition concentration (EC 50) is 56.71 mug/mL. Meanwhile, ha Erjian can remarkably inhibit the biosynthesis of DON toxin, the inhibition rate of DON synthesis can reach 25.04% unde