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CN-121970780-A - Biological control preparation for inhibiting growth of fusarium graminearum and controlling wheat scab as well as preparation method and application thereof

CN121970780ACN 121970780 ACN121970780 ACN 121970780ACN-121970780-A

Abstract

The invention relates to the technical field of biology, in particular to a biological control preparation for inhibiting growth of fusarium graminearum and controlling wheat scab, and a preparation method and application thereof. Specifically comprises myxococcus orange, pseudomonas aeruginosa, bacillus pumilus, salt-tolerant bacillus, penicillium lilacinum, rose pelargonium essential oil, herba Cephalanoploris extract, fructus cnidii extract, matrine, licorice extract, chitin oligosaccharide and pullulan. According to the invention, a multi-target synergistic biocontrol system is constructed by compounding myxococcus orange, pseudomonas aeruginosa, bacillus pumilus, salt-tolerant bacillus, penicillium purplish and various plant active ingredients. The preparation can inhibit the growth of fusarium graminearum with high efficiency, remarkably reduce toxin synthesis, effectively induce wheat resistance, has a field control effect equivalent to that of chemical agents, is environment-friendly and not easy to generate drug resistance, and provides a new scheme for green control of wheat scab.

Inventors

  • JI FANG

Assignees

  • 江苏农林职业技术学院

Dates

Publication Date
20260505
Application Date
20260128

Claims (10)

  1. 1. The biological control preparation for inhibiting the growth of fusarium graminearum and controlling wheat scab is characterized by comprising, by mass, 18-23 parts of myxococcus orange, 10-18 parts of pseudomonas aeruginosa, 12-20 parts of bacillus pumilus, 5-10 parts of salt-tolerant bacillus, 6-12 parts of penicillium lilacinum, 3-8 parts of rose geranium essential oil, 5-12 parts of herba Cephalanoploris extract, 4-10 parts of fructus cnidii extract, 0.4-0.7 part of matrine, 2-6 parts of licorice extract, 5-10 parts of chitin oligosaccharide and 5-10 parts of pullulan polysaccharide.
  2. 2. The biocontrol formulation of claim 1, wherein the colistin orange, pseudomonas aeruginosa, bacillus pumilus and salt tolerant bacillus are all 4-6 hundred million/g in bacterial content, and the spore content of penicillium microviolet is (6-9) x 10 9 CFU/g.
  3. 3. The biocontrol formulation of claim 1, wherein the preparation process of the herba Cephalanoploris extract is: (1) Taking whole grass of herba Cephalanoploris, crushing and sieving with a 10-30 mesh sieve; (2) Adding the raw materials obtained in the step (1) into an ethanol solution with the volume concentration of 60% -75% according to the feed-liquid ratio of 1:8-1:12 (w/v), and carrying out thermal reflux extraction at 70-80 ℃ for 1.5-2.5 h each time, wherein the total extraction time is 2-3 times; (3) Combining the extracting solutions obtained in the step (2), filtering, concentrating the filtrate under reduced pressure until the relative density is 1.05-1.15, and eluting; (4) Concentrating the eluent obtained in the step (3), and drying into powder to obtain the herba Cephalanoploris extract.
  4. 4. The biocontrol agent according to claim 3, wherein the elution method of step (3) is characterized by loading the concentrated solution to an HPD-100 type macroporous adsorption resin column at a flow rate of 1-3 bed volumes/h, washing with 3-5 bed volumes of water, 2-4 bed volumes of 10% -30% ethanol, eluting with 4-6 bed volumes of 60% -75% ethanol in sequence, and collecting the eluate.
  5. 5. The biocontrol formulation of claim 1, wherein the cnidium fruit extract is prepared by the process of: a. Crushing fructus cnidii fruits and sieving the crushed fructus cnidii fruits with a 30-50-mesh sieve; b. C, adding cyclohexane into the raw materials obtained in the step a according to a feed liquid ratio of 1:10-1:15 (w/v), and extracting for 2-3 times at 40-60 ℃ for 30-60 min; c. and d, merging the extracting solutions obtained in the step b, filtering, concentrating the filtrate, and drying the filtrate into powder to obtain the cnidium fruit extract.
  6. 6. The biocontrol formulation of claim 5, wherein ultrasound is required during the extraction in step b, the ultrasound power is 300-500 w and the frequency is 22-28 khz.
  7. 7. The biocontrol preparation of claim 1, wherein the preparation method of the licorice extract is characterized by adding licorice into 70-75% ethanol solution according to a feed liquid ratio of 1:8-1:12 (w/v), reflux-extracting at 80-85 ℃ for 1.5-2.5 h for 2-3 times, combining the extracts, concentrating under reduced pressure to recover ethanol, precipitating with low temperature water, filtering, concentrating the filtrate, and drying to obtain the licorice extract.
  8. 8. The biocontrol formulation of claim 7, wherein the low temperature water sink is 4-6 ℃ for 20-28 h.
  9. 9. The method for preparing the biological control agent for inhibiting the growth of fusarium graminearum and preventing and controlling wheat scab according to any one of claims 1 to 8, wherein the biological control agent for inhibiting the growth of fusarium graminearum and preventing and controlling wheat scab is prepared by mixing myxococcus orange, pseudomonas aeruginosa, bacillus pumilus, salt-tolerant bacillus, penicillium micranthum, geranium essential oil, herba Cephalanoploris extract, fructus cnidii extract, matrine, licorice extract, chitin oligosaccharide and pullulan.
  10. 10. The use of the biological control agent for inhibiting the growth of fusarium graminearum and controlling wheat scab according to any one of claims 1 to 8 in the preparation of a wheat scab control agent.

Description

Biological control preparation for inhibiting growth of fusarium graminearum and controlling wheat scab as well as preparation method and application thereof Technical Field The invention relates to the technical field of biology, in particular to a biological control preparation for inhibiting growth of fusarium graminearum and controlling wheat scab, and a preparation method and application thereof. Background Wheat scab is a global serious fungal disease caused by fusarium graminearum, and seriously threatens the yield and quality of wheat. The pathogenic bacteria not only infects the ear part to cause direct yield reduction, but also can accumulate mycotoxins such as deoxynivalenol and the like in the seeds, thereby forming serious threat to human and animal health. At present, chemical bactericides such as benzimidazole, sterol demethylating inhibitors and the like are mainly relied on for prevention and treatment, but the long-term single use of the chemical bactericides leads to increasingly prominent pathogen resistance, and the chemical bactericides have the problems of pesticide residues, ecological environment pressure and the like. Development of efficient, green and sustainable alternative prevention and control strategies has become an urgent need to ensure grain safety and agricultural high-quality development. Biological control is considered as an effective way to solve the above problems because of its advantages such as environmental friendliness and resistance generation resistance. Current research focuses on the development and use of single antagonistic strains (e.g., bacillus, trichoderma) or single plant extracts. However, the field environment is complex, and the biocontrol agent with a single action mode often shows bottlenecks of unstable control effect, short duration of effect, limited toxin control capability and the like. How to construct a multi-layer and multi-target cooperative prevention and control system through scientific component design becomes a key scientific problem for improving the efficiency of the biocontrol product. In the prior art, the compatibility of biocontrol agents is mostly based on simple combinations of common strains and known plant active ingredients, such as the compounding of bacillus subtilis and flavonoid extracts, the action mechanism is relatively single, and an effective blocking strategy for the synthesis and accumulation of fusarium graminearum toxins is lacking. Meanwhile, the plant extract obtained by the conventional extraction process (such as hot reflux and dipping) may have the problems of low extraction rate of active ingredients, more impurities and the like, and the compatibility and stability of the plant extract in the compound preparation are affected. More importantly, when the plant extract with strong antibacterial activity is directly compounded with the live bacteria prebiotics, antagonism possibly existing between the plant extract and the live bacteria prebiotics is usually ignored, so that the survival rate of the live bacteria is reduced, and the comprehensive efficiency of the preparation is greatly reduced. Therefore, there is a need to develop a new biocontrol agent, which is characterized in that atypical probiotic microorganisms with unique niches and action mechanisms are screened, and specific plant source active ingredients which are extracted in an optimized way are matched. The preparation can synergistically exert multiple functions of inhibiting the growth of pathogenic bacteria, interfering toxin synthesis, inducing plant resistance and promoting the growth of crops, thereby realizing green, efficient and sustainable treatment of wheat scab. Disclosure of Invention The invention aims to provide a biological control preparation for inhibiting growth of fusarium graminearum and preventing and controlling wheat scab, and a preparation method and application thereof. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a biological control preparation for inhibiting growth of fusarium graminearum and controlling wheat scab, which comprises, by mass, 18-23 parts of myxococcus orange, 10-18 parts of pseudomonas aeruginosa, 12-20 parts of bacillus pumilus, 5-10 parts of salt-tolerant bacillus, 6-12 parts of penicillium lilacinum, 3-8 parts of rose geranium essential oil, 5-12 parts of herba Cephalanoploris extract, 4-10 parts of fructus cnidii extract, 0.4-0.7 part of matrine, 2-6 parts of licorice extract, 5-10 parts of chitin oligosaccharide and 5-10 parts of pullulan polysaccharide. Preferably, the bacteria content of the myxococcus orange, the pseudomonas aeruginosa, the bacillus pumilus and the salt-tolerant bacillus are all 4-6 hundred million/g, and the spore content of the penicillium microviolet is (6-9) multiplied by 10 9 CFU/g. Preferably, the preparation method of the herba Cephalanoploris extract comprises the following steps