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CN-121970781-A - Application of trichoderma harzianum in preventing and treating potato black nevus and scab

CN121970781ACN 121970781 ACN121970781 ACN 121970781ACN-121970781-A

Abstract

The disclosure relates to the use of trichoderma harzianum for the control of potato black nevus and scab. Trichoderma harzianum with the preservation number of CGMCC No.40017 can well inhibit rhizoctonia solani so as to achieve the effect of preventing and treating potato black nevus, and trichoderma harzianum with the preservation number of CGMCC No.40017 can also inhibit streptomyces, such as streptomyces scab, streptomyces griseus and the like so as to achieve the effect of preventing and treating potato scab.

Inventors

  • WU BEILEI
  • DING MENG
  • LIU XINAO
  • JIANG XILIANG
  • LI MEI
  • MEI JIE
  • LIU CHENCHEN
  • WANG RONGQUN
  • TAN ZHAOYAN
  • CAI WENXIN
  • ZHAO YUHUI

Assignees

  • 中国农业科学院植物保护研究所

Dates

Publication Date
20260505
Application Date
20251230

Claims (8)

  1. 1. The application of trichoderma harzianum in the aspect of preventing and treating potato black nevus and scab is characterized in that the classification of trichoderma harzianum is named trichoderma harzianum Trichoderma harzianum, and the preservation number of trichoderma harzianum is CGMCC No.40017.
  2. 2. The use according to claim 1, wherein the use comprises applying trichoderma harzianum to the soil of a potato plant.
  3. 3. The use of claim 2, wherein the application comprises any one of spraying, root irrigation and soil mixing.
  4. 4. The use according to claim 2, wherein the trichoderma harzianum is used in an amount of 10 7 -10 11 CFU/strain.
  5. 5. The use according to claim 1, wherein the use comprises applying a microbial inoculant comprising trichoderma harzianum with a preservation number of CGMCC No.40017 to the soil of a potato plant.
  6. 6. The use of claim 5, wherein the application comprises any one of spraying, root irrigation, and soil mixing.
  7. 7. The use according to claim 5, wherein the viable count of trichoderma harzianum with a preservation number of CGMCC No.40017 is 10 7 -10 11 CFU per gram of the microbial agent.
  8. 8. The use according to claim 5, wherein the microbial agent is used in an amount of 1-2 kg/mu.

Description

Application of trichoderma harzianum in preventing and treating potato black nevus and scab Technical Field The disclosure relates to the technical field of microorganisms, in particular to application of trichoderma harzianum in preventing and treating potato black nevus and scab. Background Potato (Solanum tuberosum l.) is an annual herb of the genus Solanum of the family solanaceae. Potato black nevus is a disease of potato caused by rhizoctonia solani, mainly in the form of unwanted buds, stem bases and tubers. The young buds are infected, and some buds are rotted into bud rot before soil emergence, so that seedling deficiency is caused. And at the beginning of the infection after the soil emergence, the leaves at the lower part of the plant turn yellow, and the stems form brown sunk spots. In severe cases, stem axillary buds produce purple or green aerial tubers, or the basal part of the underground stem produces many small potatoes without economic value, and many black brown sclerotium are scattered on the surface. Serious disease occurs, and the incidence rate of the severe field plants reaches 70-80%. The potato yield can be reduced by more than 15% in the common year, the whole field can be destroyed in the individual year, and the yield and quality of the potato can be seriously affected. Potato scab is one of the important diseases of potatoes in the world, and is caused by various streptomyces species and occurs in potatoes. Is ubiquitous in China potato production bases. After the disease occurs, although the disease spots are limited to the cortex, the disease potatoes are not storage-resistant and have an unsightly appearance. The quality of the potatoes is greatly affected after the disease is felt, so that the commodity value is reduced, and the yield can be generally reduced by 10-30%, and the yield of partial plots is reduced by more than 40%. Mainly damages tubers, brown spots are generated on the surfaces of initial tubers, the brown spots are gradually enlarged to be nearly round or irregular large spots, and the spots are polydisperse. The disease spots are in reticulate patterns and split patterns, the epidermis is roughened and lignified, the edge of the disease spots is raised after cracking, the center of the disease spots is depressed, and the disease spots are rust-colored, dark brown or black scab-shaped hard spots. Therefore, there is a need to develop a safe biocontrol product. Disclosure of Invention The purpose of the disclosure is to provide the application of trichoderma harzianum in preventing and treating potato black nevus and scab. The disclosure provides application of trichoderma harzianum in preventing and treating potato black nevus and scab, wherein the classification of trichoderma harzianum is named trichoderma harzianum Trichoderma harzianum, and the preservation number of trichoderma harzianum is CGMCC No.40017. Alternatively, the application comprises applying trichoderma harzianum to the soil of a potato plant. Optionally, the applying includes any one of spraying, root irrigation and soil mixing. Optionally, the trichoderma harzianum is used in an amount of 10 7-1011 CFU/strain. Alternatively, the application comprises applying a microbial inoculant containing trichoderma harzianum with the preservation number of CGMCC No.40017 to the soil of a potato plant. Optionally, the applying includes any one of spraying, root irrigation and soil mixing. Optionally, the viable count of trichoderma harzianum with the preservation number of CGMCC No.40017 in each gram of the microbial agent is 10 7-1011 CFU. Optionally, the dosage of the microbial agent is 1-2 kg/mu. According to the technical scheme, trichoderma harzianum with the preservation number of CGMCC No.40017 can well inhibit rhizoctonia solani so as to achieve the effect of preventing and treating potato black nevus, trichoderma harzianum with the preservation number of CGMCC No.40017 can also inhibit streptomyces, such as streptomyces scab, streptomyces griseus and other streptomyces, so that the effect of preventing and treating potato scab is achieved, and the trichoderma harzianum has important significance in agricultural production. Additional features and advantages of the present disclosure will be set forth in the detailed description which follows. Preservation of biological materials The inventors of the present disclosure isolated and cultured a new strain from a soil sample of cocoa tuoha in Fuyun county of alenta, xinjiang, identified as belonging to the genus Trichoderma (Trichoderma spp.), designated Trichoderma harzianum Trichoderma harzianum. The strain is preserved in China general microbiological culture Collection center of preservation unit designated by national intellectual property agency, the preservation date is 2021, 12 months and 28 days, and the preservation number is CGMCC No.40017. Drawings The accompanying drawings are included to provide a further understanding of the disc