Search

CN-121970808-A - Apigenin-chitosan composite coating liquid and preparation method and application thereof

CN121970808ACN 121970808 ACN121970808 ACN 121970808ACN-121970808-A

Abstract

The invention discloses apigenin-chitosan composite coating liquid and a strawberry preservation method, and belongs to the technical field of fruit and vegetable postharvest preservation. The apigenin-chitosan composite coating liquid comprises the following components of chitosan or derivatives thereof, apigenin or apigenin-containing extract and acidic aqueous solution, wherein the mass volume concentration of apigenin or apigenin-containing extract in the composite coating liquid is 0.1-0.3% (w/v), and the mass volume concentration of chitosan in the composite coating liquid is 1.0-1.5% (w/v). The coating liquid is prepared by physically blending apigenin with specific concentration and chitosan. When the special proportion is used for strawberry film coating preservation, the synergistic effect between apigenin and chitosan can be triggered, so that the weight loss rate and the decay index of strawberries in the storage period are obviously reduced, and the vitamin C content, the total acid content and the fruit hardness are effectively maintained. The invention has natural raw materials and simple process, and provides apigenin-chitosan composite coating liquid and a strawberry preservation method.

Inventors

  • ZHAO HAIJIE
  • LI ZIMU

Assignees

  • 东北农业大学

Dates

Publication Date
20260505
Application Date
20260210

Claims (9)

  1. 1. The apigenin-chitosan composite coating liquid is characterized by comprising the following components of chitosan or derivatives thereof, apigenin or apigenin-containing extract and acidic aqueous solution, wherein the mass volume concentration of apigenin or apigenin-containing extract in the composite coating liquid is 0.1-0.3% (w/v), and the mass volume concentration of chitosan in the composite coating liquid is 1.0% -1.5% (w/v).
  2. 2. The composite coating liquid according to claim 1, wherein the chitosan or a derivative thereof has a degree of deacetylation of not less than 70%.
  3. 3. The composite coating liquid according to claim 1, wherein the acidic aqueous solution is any one of an aqueous solution of lactic acid, an aqueous solution of citric acid, an aqueous solution of ascorbic acid, and an aqueous solution of acetic acid.
  4. 4. The composite coating liquid according to claim 1, wherein the volume concentration of the acidic aqueous solution is 1%.
  5. 5. The composite coating liquid according to claim 1, wherein the chitosan derivative is any one of carboxymethyl chitosan, chitosan hydrochloride or chitosan quaternary ammonium salt.
  6. 6. A method for producing the composite coating liquid according to any one of claims 1 to 5, comprising the steps of: s1, dissolving chitosan or a derivative thereof in an acidic aqueous solution to obtain a film-forming base solution; S2, dissolving apigenin or an apigenin-containing extract in absolute ethyl alcohol to obtain an active substance stock solution; and S3, mixing the active material stock solution obtained in the step S2 with the film forming base solution obtained in the step S1, and carrying out constant volume and stirring to obtain the composite coating liquid.
  7. 7. The use of the composite coating liquid according to any one of claims 1 to 5 for coating preservation treatment of strawberries.
  8. 8. The application of the coating film according to claim 7, wherein the coating film fresh-keeping treatment comprises immersing the strawberries in the composite coating film liquid for 3-8 minutes, taking out and drying to form the composite fresh-keeping film on the surfaces of the strawberries.
  9. 9. A preservative film formed by coating and drying the composite coating liquid according to any one of claims 1 to 5.

Description

Apigenin-chitosan composite coating liquid and preparation method and application thereof Technical Field The invention belongs to the technical field of fruit and vegetable postharvest preservation, and particularly relates to apigenin-chitosan composite coating liquid, and a preparation method and application thereof. Background The strawberry has thin pericarp, tender tissue, vigorous respiration metabolism after harvest, and is extremely easy to decay rapidly due to mechanical damage and microorganism infection, and the normal temperature shelf life is usually only 3-5 days, so that huge postharvest loss is caused. In order to prolong the shelf life, the traditional chemical preservation method has the harmful residual risk, and the physical preservation method has the disadvantages of expensive equipment and complex operation, and is difficult to meet the green, safe and economic industrial requirements. In recent years, edible film coating technology using chitosan as a substrate has become a research hot spot due to its good film forming property, biocompatibility and natural antibacterial property. The deacetylation degree of chitosan is a key parameter, and generally, the higher the deacetylation degree is, the more free amino groups on a molecular chain are, the stronger the antibacterial activity and the film forming performance are, however, the pure chitosan film has the inherent defects of weak mechanical strength, poor water vapor barrier property, limited antibacterial spectrum and the like, and the fresh-keeping effect is insufficient when the pure chitosan film is singly used. For this reason, the prior art generally adopts a strategy of adding natural active ingredients to chitosan for modification. For example, peppermint essential oil (0.5% -1.5%, v/v) is dispersed in chitosan acetic acid solution through an emulsifier (such as tween-80) to form a composite coating film for fresh-keeping of purple sweet potato (see non-patent document 1: liu Xu, etc., fresh-keeping effect of essential oil-chitosan composite coating film on purple sweet potato). Similarly, techniques for compounding other plant essential oils (e.g., carvacrol, tea tree oil) or complex plant extracts with chitosan or its derivatives (e.g., quaternized chitosan) have been reported (see non-patent document 2: chen Li et al ,"Green and facile fabrication of multifunctional cellulose nanocrystal and carvacrol together reinforced chitosan bio-nanocomposite coatings for fruit preservation"、 non-patent document 3: ying Liu et al ,"High-adhesion antimicrobial composite coating incorporating quaternary chitosan and tea tree oil for enhanced preservation of fruits and vegetables"）. However, there are significant limitations to the prior art: 1. The active ingredients are limited in selection, and plant essential oil or mixed extract with complex ingredients, easy volatilization and unstable properties are commonly used, so that the fresh-keeping effect is large in fluctuation and poor in durability, and peculiar smell can be introduced to influence the fruit flavor. 2. The proportion optimization is extensive in that the addition amount of the active ingredients is set to a wider range based on experience, the research emphasis is on verifying whether an effect exists or not, a system for optimal synergistic proportion is lacking, fine exploration is performed, and a possible nonlinear relation between the concentration and the effect is not revealed. 3. Most of the research stays at the verification level of the overall effect of the composite membrane, and the optimum synergistic condition between specific active ingredients and chitosan matrix is lack of deep excavation. Therefore, a new technology for fresh-keeping coating film based on optimized proportion, stable effect and all natural components is needed to be developed in the field. Disclosure of Invention The invention aims to overcome the defects of the prior art and solve the problems of unstable and non-durable fresh-keeping effect and the like caused by improper selection and non-optimal proportion of active ingredients of the traditional chitosan composite fresh-keeping coating film. In particular to an optimal proportioning range of apigenin-chitosan composite coating liquid, thereby realizing an all-natural coating with high efficiency, durability and synergistic fresh-keeping. In order to achieve the above purpose, the present invention provides the following technical solutions: the apigenin-chitosan composite coating liquid comprises chitosan or a derivative thereof, apigenin or an apigenin-containing extract and an acidic aqueous solution, wherein the mass volume concentration of apigenin or apigenin-containing extract in the composite coating liquid is 0.1-0.3% (w/v), and the mass volume concentration of chitosan in the composite coating liquid is 1.0-1.5% (w/v). The deacetylation degree of the chitosan or the derivative thereof is not less than 70%. The acidi