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CN-121970826-A - Preparation and application of carbon emission reduction composition sustained release preparation for ruminant farm

CN121970826ACN 121970826 ACN121970826 ACN 121970826ACN-121970826-A

Abstract

The application belongs to the field of biological feed, and discloses application of large listeria in preparing an additive for inhibiting generation of greenhouse gases in animal feces, wherein the large listeria is preserved in the microorganism strain preservation center of Guangdong province at 18 days of 2025, and the preservation number is GDMCC NO:66160. The large Listeria megaterium has high-efficiency enzyme producing capability, and after the large Listeria megaterium is planted in the rumen, cellulose is decomposed efficiently, so that carbon emission of ruminants is reduced obviously. Meanwhile, the application further uses probiotics with various enzyme production characteristics and propionibacteria to compound, and the obtained intestinal probiotics composition has obvious synergistic effect and obviously reduces carbon emission. Finally, the application also provides a composition containing fermentation products, the intestinal probiotics composition and the microcapsule with the insect grease slow-release function, which can obviously reduce the carbon emission of ruminants.

Inventors

  • WANG CHUNQUAN
  • RONG TING
  • XU TINGSEN
  • ZHOU HANNI
  • OU GUANHUA
  • LUO LANHUA
  • ZHANG LIMIN
  • Zhu Pinsong

Assignees

  • 广州汇旺生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260206

Claims (10)

  1. 1. Use of a large listeria bacterium deposited at the microorganism strain deposit center in the Guangdong province at month 4 of 2025 under the deposit number GDMCC NO:66160 for the preparation of an additive for inhibiting the production of greenhouse gases in animal feces.
  2. 2. Use according to claim 1, characterized in that the greenhouse other is methane or carbon dioxide.
  3. 3. The intestinal probiotics composition for reducing carbon emission of ruminants is characterized by comprising propionibacteria, bacillus bailii XY1 and Listeria megaterium, wherein the viable bacteria ratio of the propionibacteria, the bacillus bailii XY1 and the Listeria megaterium is 1-5:1-5.
  4. 4. A composition slow release preparation for reducing carbon emission of ruminants, which is characterized by comprising the following components in parts by weight: 20-30 parts of Aspergillus niger fermentation product and/or Aspergillus oryzae enzyme fermentation product; The intestinal probiotic composition of claim 2, wherein the viable bacteria content of the intestinal probiotic composition is 1.1X10 9 ~1.5×10 9 cfu/g; 1-5 parts of slow release microcapsules; The slow-release microcapsule comprises a shell material composed of sodium alginate, chitosan and tannic acid and insect grease coated in the shell material.
  5. 5. The composition slow release preparation according to claim 4, wherein the weight ratio of the shell material of the slow release microcapsule to the insect fat is 10-15:20-40.
  6. 6. The sustained-release preparation of a composition according to claim 5, wherein the preparation method of the sustained-release microcapsule comprises: step1, heating chitosan, insect grease, an emulsifier and water to 50-60 ℃ and stirring to form emulsion; Step 2, adding the sodium alginate aqueous solution into the emulsion in the step 1, stirring, and homogenizing after stirring is finished; and step 3, dropwise adding tannic acid into the system in the step 3, solidifying sodium alginate and chitosan, standing to form layering, removing supernatant to obtain microcapsule powder, and drying.
  7. 7. The composition sustained-release preparation according to claim 6, wherein the mass ratio of the chitosan to the sodium alginate is 1:0.8-1.2, the mass ratio of the chitosan to the tannic acid is 1:0.05-0.15, the stirring speed is 500-1000 rpm, the homogenizing speed is 6000-10000 rpm, and the homogenizing time is 3-5 min.
  8. 8. The composition slow-release preparation according to claim 6, wherein the emulsifier is tween-80 or tween-60, the amount of the emulsifier is 2-5% of the amount of insect grease, and the insect grease is hermetia illucens larva grease.
  9. 9. Use of a sustained release formulation of a composition as claimed in any one of claims 4 to 8 in the manufacture of an additive for inhibiting the production of greenhouse gases in animal litter.
  10. 10. An additive for inhibiting the production of greenhouse gases in animal manure, characterized in that an intestinal probiotic composition according to claim 2 or a slow release formulation of a composition according to any one of claims 4 to 8 is added.

Description

Preparation and application of carbon emission reduction composition sustained release preparation for ruminant farm Technical Field The application relates to the field of biological feeds, in particular to a new application of Listeria megaterium, an intestinal probiotic composition and an emission reduction composition. Background The use of Listeria megaterium is mostly for soil remediation such as contribution to plant growth in saline-alkali soil, or pollutant decomposition, and the following information is found in the relevant literature: The publication number is CN120536293A, the subject is a large Listeria and the application thereof in the treatment of pig farm manure, and the application of the large Listeria in the reduction of pig farm wastewater is described. The inventor firstly provides a patent application with the publication number of CN120836648A, and mainly aims at a method for efficiently preparing earthworm functional liquid by utilizing endogenous probiotics and enzymes in the intestinal tract of earthworms and the earthworm functional liquid, and the method utilizes the efficient protease production capacity of the large Listeria for decomposing earthworm proteins to produce small peptides. In further research on the large listeria, we found that the large listeria has obvious effect in the field of cultivation, and further put forward the invention. Disclosure of Invention One of the purposes of the application is to provide a new application of the giant listeria, which has high-efficiency enzyme production capacity and can effectively reduce carbon emission of ruminants after the giant listeria is planted in intestinal tracts. In general, carbon emissions from ruminants originate primarily from the rumen and the intestine, and the probiotics of the present invention are primarily used to reduce carbon emissions produced by the intestine. Meanwhile, the invention further uses probiotics with various enzyme producing characteristics and propionibacteria to combine, and the obtained intestinal probiotics composition has a more obvious function of reducing carbon emission. Finally, the invention also provides a composition containing fermentation products, the intestinal probiotics composition and the microcapsule with the insect grease slow-release function, which can obviously reduce the carbon emission of ruminants. In order to achieve the aim, the application provides the application of the giant listeria in preparing intestinal probiotics or feed additives, wherein the intestinal probiotics or feed additives are used for reducing carbon emission of ruminants, and the giant listeria is preserved in the microorganism strain preservation center of Guangdong province at the month of 4 days 2025 and the preservation number is GDMCC NO:66160. In addition, the invention also provides an intestinal probiotic composition for reducing carbon emission of ruminants, which consists of propionibacteria, bacillus bailii XY1 and Listeria megaterium, wherein the viable bacteria ratio of the propionibacteria, the bacillus bailii XY1 and the Listeria megaterium is 1-5:1-5. Meanwhile, the invention also provides a composition sustained release preparation for reducing carbon emission of ruminants, which comprises the following components in parts by weight: 20-30 parts of Aspergillus niger fermentation product and/or Aspergillus oryzae enzyme fermentation product; 1-2 parts of the intestinal probiotic composition, wherein the living bacteria content of the intestinal probiotic composition is 1.1X10 9~1.5×109 cfu/g, preferably 1.3X10 9~1.5×109 cfu/g. 1-5 Parts of slow release microcapsules; The slow-release microcapsule comprises a shell material composed of sodium alginate, chitosan and tannic acid and insect grease coated in the shell material. In the composition slow release preparation, the weight ratio of the shell material of the slow release microcapsule to the insect grease is 10-15:20-40. In the composition slow release preparation, the preparation method of the slow release microcapsule comprises the following steps: step1, heating chitosan, insect grease, an emulsifier and water to 50-60 ℃ and stirring to form emulsion; Step 2, adding the sodium alginate aqueous solution into the emulsion in the step 1, stirring, and homogenizing after stirring is finished; And step 3, dropwise adding tannic acid into the system in the step 2, solidifying sodium alginate and chitosan, standing to form layering, removing supernatant to obtain microcapsule powder, and drying. In the composition slow release preparation, the mass ratio of the chitosan to the sodium alginate is 1:0.8-1.2, the mass ratio of the chitosan to the tannic acid is 1:0.05-0.15, the stirring speed is 500-1000 rpm, the homogenizing speed is 6000-10000 rpm, and the homogenizing time is 3-5 min. In the composition slow release preparation, the emulsifier is tween-80 or tween-60, the dosage of the emulsifier is 2-5% of that of insect gr