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CN-121970827-A - Composite probiotic composition with biodegradation function on hair bulbs and application thereof

CN121970827ACN 121970827 ACN121970827 ACN 121970827ACN-121970827-A

Abstract

The invention belongs to the technical field of pet foods and biological medicines, and discloses a composite probiotic composition with a biodegradation function and application thereof. The composition comprises an extract of Welch's mannheimia coagulans, bacillus subtilis and Camellia oleifera seed. According to the invention, the camellia seed extract from a specific source is used as a biosurfactant to emulsify and break the oil seal sebum layer on the surface of the fur of the pet, so that the surface tension of the hair is obviously reduced, the keratinase secreted by bacillus subtilis is induced to efficiently moisten and adsorb on hair fibers, the deep biodegradation of the keratins is realized, and meanwhile, the lactic acid produced by the condensation of the Wittman's bacteria is combined to promote intestinal peristalsis to cooperatively realize the disintegration and excretion of the hair bulb. Experimental results show that the composition has 65% -78% loss rate of dry weight of cat hair, can effectively reduce vomiting frequency, and has excellent acid-resistant stability, palatability and safety.

Inventors

  • ZHAO BAIWEN
  • XU WENYI
  • ZHANG BOCHENG
  • ZANG TAO
  • KANG GUOKAI
  • WANG YANXIN
  • ZHAO YUJIE

Assignees

  • 北京量化健康科技有限公司

Dates

Publication Date
20260505
Application Date
20260403

Claims (9)

  1. 1. A composite probiotic composition with the effect of biodegrading hair bulb comprises a condensation Wittman bacterium (Weizmannia coagulans), a bacillus subtilis (Bacillus subtilis) and an oil tea seed extract, wherein the collection number of the condensation Wittman bacterium is CGMCC No. 35843, and the collection number of the bacillus subtilis is CGMCC No. 35844.
  2. 2. The composite probiotic composition of claim 1, wherein the ratio of viable count of the bacillus subtilis to the manchurian condensation bacteria is 2:1-5:1.
  3. 3. The composite probiotic composition of claim 1, wherein the tea saponin content of the camellia seed extract is 60% -70% by weight.
  4. 4. The composite probiotic composition according to claim 1, wherein the composition comprises 10-20 parts of the powder of the Willebrand bacteria, 3-10 parts of the powder of the bacillus subtilis and 1-5 parts of the extract of the camellia seeds in parts by weight.
  5. 5. The complex probiotic composition according to any one of claims 1 to 4, further comprising a protective agent selected from one, two or more of goat milk powder, maltodextrin, trehalose, skimmed milk powder.
  6. 6. A composite particle comprising a core material and a wall material, wherein the core material comprises the composition of any one of claims 1-5, the wall material comprises sodium alginate and whey protein, the composite particle is a microcapsule-embedded particle, and the moisture content of the microcapsule-embedded particle is less than or equal to 4.5%.
  7. 7. A process for the preparation of a composition as claimed in any one of claims 1 to 5, comprising the steps of: (1) Culturing the Wittman's bacterium coagulans and bacillus subtilis respectively, and preparing and obtaining bacterial powder with the number of effective viable bacteria not lower than 1X 10 11 CFU/g; (2) Preparing an oil tea seed extract with tea saponin content of more than or equal to 60% by adopting an ethanol ultrasonic extraction process; (3) Mixing the bacterial powder with the camellia seed extract to obtain the composition.
  8. 8. A method of preparing composite particles comprising the steps of: (1) Culturing the Wittman's bacterium coagulans and bacillus subtilis respectively, and preparing and obtaining bacterial powder with the number of effective viable bacteria not lower than 1X 10 11 CFU/g; (2) Preparing an oil tea seed extract with tea saponin content of more than or equal to 60% by adopting an ethanol ultrasonic extraction process; (3) Mixing the bacterial powder with the camellia seed extract to obtain a core material; (4) The core material coats the wall material to prepare composite particles with the moisture content less than or equal to 4.5%, and the wall material comprises sodium alginate and whey protein.
  9. 9. The use of the composite probiotic composition of any one of claims 1 to 5, or the composite particles of claim 6, for the preparation of pet grooming products, which are pet foods or pet health products, including pet dry foods, baked foods, wet foods cans, grooming pastes or grooming Mao Yisheng powders.

Description

Composite probiotic composition with biodegradation function on hair bulbs and application thereof Technical Field The invention belongs to the technical field of pet foods and biological medicines, and particularly relates to composite particles containing extracts of Wemtman coagulans, bacillus subtilis and camellia seed, and application thereof in promoting degradation and excretion of hair balls of pets. Background Pets such as cats and rabbits have the habit of licking the fur. After being taken into the digestive tract, keratin (Keratin) is extremely difficult to be decomposed by digestive enzymes and is extremely easy to be entangled in the gastrointestinal tract to form hair balls (Trichobezoars). The hairball not only causes vomiting and inappetence, but also causes intestinal obstruction (hairball disease) in severe cases, and even endangers life. At present, commercial hair-melting products are mainly divided into two types, namely physical hair-removing products, wherein dietary fibers (such as semen plantaginis and catweed) are mainly added to increase intestinal peristalsis, but the formed compact hair balls lack decomposition capability, and mineral oil/grease is used for lubricating the intestinal tracts, but the absorption of fat-soluble vitamins (such as VA, D and E) by pets is interfered by long-term consumption, and non-natural ingredients do not meet the health requirements of modern pets. In an attempt to solve the hair bulb disease by biotechnology, the use of probiotics such as bacillus subtilis to secrete keratinase for biodegradation of hair bulbs has become a research hotspot. However, practical application shows that the degradation effect of the pure bacillus subtilis on the pet hair bulb is obviously limited. This is because the hydrophobic sebum layer on the surface of the pet's coat acts as a ' oil seal ' (i.e., a highly hydrophobic sebum shield layer on the surface of the coat) that blocks contact of the water-soluble keratinase with hair keratin. In the extremely limited intestinal transit time (usually 12-24 hours) of pets, the enzyme liquid produced by bacillus subtilis cannot break through the grease barrier, so that the degradation rate of the bacillus subtilis on compact hair bulbs is extremely low. On the other hand, wettman coagulans is often used to regulate the intestinal motility of pets, and the lactic acid produced by the Wettman coagulans moderately stimulates the smooth muscle peristalsis of the intestinal tract. However, when treating intractable hair bulb diseases, the simple use of the condensed Wittman's bacteria has obvious defects that the hair bulb can only be pushed to move from a physical level, but the physical entanglement structure of the hair bulb cannot be damaged. For the formed hair bulb with larger volume, the pure enhancement of intestinal power is difficult to smoothly discharge, and even the risk of intestinal mucosa injury or increased obstruction can be caused by the friction between strong peristaltic motion and the hard hair bulb. In addition, if the two probiotics are simply and physically mixed for use, although 'chemical' and 'arrangement' can be achieved to a certain extent, the enzyme production efficiency of bacillus subtilis is still greatly inhibited due to the lack of biochemical means for breaking the hair grease barrier, so that the embarrassing situation of '1+1<2' is presented to the overall hair effect. Therefore, how to find a comprehensive scheme capable of synergistically enhancing the degradation efficiency of probiotics, breaking through the grease obstruction and safely discharging hairs is a technical problem to be solved in the field of pet health at present. Further studies have found that a deep cause of the inhibition of the efficiency of the probiotics to fuzz is due to the microscopic physicochemical properties of the surface of the hair bulb. Because the grease (such as cholesterol ester, free fatty acid and the like) secreted by the sebaceous glands of the cats is tightly wrapped on the outer edge of the flaked layer of the hair, a highly hydrophobic film is formed. The 'oil film' not only ensures that water-soluble keratinase cannot directly contact a substrate, but also forms extremely high surface tension between intestinal fluid and hair bulb, so that effective components generated by metabolism of probiotics are 'repelled' outside the hair bulb structure. Although chemical synthetic surfactants (such as sodium lauryl sulfate) are often used in the field of industrial cleaning or leather treatment to remove grease, such ingredients have strong cytotoxicity and mucous membrane irritation and are never used for internal administration to pets. However, the natural emulsifying agent (such as soybean lecithin) commonly used in the field of pet foods is unstable in complex intestinal acid-base environments, and has difficulty in generating sufficient wetting force to penetrate through compact hair entanglemen